Evaluation of chitosan/β-tricalcium phosphate microspheres as a constituent to PMMA cement

Abstract

Two methods, a traditional emulsion technique and a high voltage electrostatically modified encapsulation system, were used to fabricate degradable chitosan/β -tricalcium phosphate (β-TCP) microspheres. The two distinct kinds of microspheres both exhibited good sphericity and the β -TCP was trapped well inside the chitosan gel. The microspheres prepared by high voltage electrostatic system exhibited a rougher outer surface and narrower size distribution. These microspheres were then used as an added constituent to commercially available PMMA bone cement. Four modified cement composites that were prepared with different composition ratios of the two kinds of chitosan/β-TCP microspheres that were made from emulsion technique (C1P1 and C2P1) and from a process by a high voltage electrostatic system (EC1P1 and EC2P1) were compared with the PMMA cement (Pure P). The characteristics of these materials indicate that with the addition of chitosan/β -TCP microspheres as a constituent into the PMMA cement significantly decreases the curing peak temperature. Furthermore, the setting time increases from 3.5-min to 9-min, as compared to the PMMA cement. These changes could be beneficial for the handling of the bone cement paste and causing less damage to the surrounding tissues. Understandably, the presence of chitosan/β -TCP microspheres in the prepared composites reduced the ultimate compressive strength and bending strength. From the degradation test and SEM observations, the modified chitosan/β -TCP/PMMA composites could be degraded gradually and create rougher surfaces that would be beneficial to cell adherence and growth.