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Dialdehyde cellulose nanocrystal/gelatin hydrogel optimized for 3D printing applications

  • Polymers
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Abstract

Three-dimensional (3D) printing, used to fabricate modular and patient-specific scaffolds with high structural complexity and design flexibility, has drawn wide attentions in the tissue engineering area. However, one of the key problems hindering the application and development of 3D printing in TE area is the poor mechanical property of bio-inks. In this work, we aimed to design a high-strength hydrogel system based on dialdehyde cellulose nanocrystals (DAC) and gelatin (GEL) as a new bio-ink for 3D printing of scaffolds. The DAC were prepared and used as a natural crosslinker to interact with the GEL through a Schiff base reaction. The mechanical test results indicated that the breaking strength of the optimal 4:8-DAC/GEL sample was almost 41.3-fold greater than that of the GEL hydrogel. According to the rheological test results, the 4:8-DAC/GEL sample incubated for 3 h was proposed as a bio-ink for 3D printing. Then, the printing conditions, including the printing pressure and nozzle speed, as well as additional crosslinking conditions for a freshly printed scaffold, i.e., the crosslinking time and temperature, were optimized. Crosslinked scaffolds with adjustable porosity and good fidelity were successfully obtained. The biocompatibility of 4:8-DAC/GEL was also investigated by CCK-8 and Hoechst 33342/PI double-staining assays. Collectively, these results confirm the good potential of the 4:8-DAC/GEL hydrogel as a 3D bio-ink for application in tissue repair.

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References

  1. Zhang YS, Yue K, Aleman J et al (2017) 3D bioprinting for tissue and organ fabrication. Ann Biomed Eng 45(1):148–163

    Google Scholar 

  2. Zhang X, Yang Y, Yao J, Shao Z, Chen X (2014) Strong collagen hydrogels by oxidized dextran modification. ACS SustaiChem Eng 2(5):1318–1324

    CAS  Google Scholar 

  3. Li H, Liu S, Lin L (2016) Rheological study on 3D printability of alginate hydrogel and effect of graphene oxide. Int J Bioprinting 2(2):54–66

    CAS  Google Scholar 

  4. Abbadessa A, Blokzijl MM, Mouser VH et al (2016) A thermo-responsive and photo-polymerizable chondroitin sulfate-based hydrogel for 3d printing applications. Carbohydr Polym 149:163–174

    CAS  Google Scholar 

  5. Christensen K, Xu C, Chai W, Zhang Z, Fu J, Huang Y (2015) Freeform inkjet printing of cellular structures with bifurcations. Biotechnol Bioeng 112(5):1047–1055

    CAS  Google Scholar 

  6. Duan B, Hockaday LA, Kang KH et al (2013) 3D bioprinting of heterogeneous aortic valve conduits with alginate/gelatin hydrogels. J Biomed Mater Res Part A 101A(5):1255–1264

    CAS  Google Scholar 

  7. Colosi C, Shin SR, Manoharan V et al (2016) Microfluidic bioprinting of heterogeneous 3d tissue constructs using low viscosity bioink. Adv Mater 28(4):677–681

    CAS  Google Scholar 

  8. Lee VK, Kim DY, Ngo H et al (2014) Creating perfused functional vascular channels using 3d bio-printing technology. Biomaterials 35(28):8092–8102

    CAS  Google Scholar 

  9. Kim Y, Lee H, Kim G (2016) Strategy to achieve highly porous/biocompatible macroscale cell blocks, using a collagen/genipin-bioink and an optimal 3D printing process. ACS Appl Mater Interfaces 8(47):32230–32240

    CAS  Google Scholar 

  10. Aleksander S, David M, Edi K et al (2012) Bioprinted amniotic fluid-derived stem cells accelerate healing of large skin wounds. Stem Cells Transl Med 1(11):792–802

    Google Scholar 

  11. Highley CB, Rodell CB, Burdick JA (2015) Direct 3d printing of shear-thinning hydrogels into self-healing hydrogels. Adv Mater 27(34):5075–5079

    CAS  Google Scholar 

  12. Muller WEG, Neufurth M, Tolba E et al (2015) A new printable and durable n, o-carboxymethyl chitosan-ca2 + -polyphosphate complex with morphogenetic activity. J Mater Chem B 3(8):1722–1730

    Google Scholar 

  13. Zhang X, Yang Y, Yao J et al (2014) Strong collagen hydrogels by oxidized dextran modification. Acs Sustain Chem Eng 2(5):1318–1324

    CAS  Google Scholar 

  14. Tan YJ, Tan X, Yeong WY et al (2016) Hybrid microscaffold-based 3D bioprinting of multi-cellular constructs with high compressive strength: a new biofabrication strategy. Sci Rep 6:39140

    CAS  Google Scholar 

  15. Kim YB, Lee H, Kim GH (2016) Strategy to achieve highly porous/biocompatible macroscale cell blocks, using a collagen/genipin-bioink and an optimal 3D printing process. Acs Appl Mater Interfaces 8(47):32230–32240

    CAS  Google Scholar 

  16. Kim T, Sridharan I, Zhu B, Effect of CNT on collagen fiber structure et al (2015) Stiffness assembly kinetics and stem cell differentiation. Mater Sci Eng C Mater Biol Appl 49:281–289

    CAS  Google Scholar 

  17. Peppas N, Hilt J, Khademhosseini A et al (2006) Hydrogels in biology and medicine: from molecular principles to bionanotechnology. Adv Mater 18(11):1345–1360

    CAS  Google Scholar 

  18. Henriksson I, Gatenholm P, Hägg DA (2017) Increased lipid accumulation and adipogenic gene expression of adipocytes in 3D bioprinted nanocellulose scaffolds. Biofabrication 9(1):015022

    CAS  Google Scholar 

  19. Piras CC, Fernándezprieto S, De Borggraeve WM (2017) Nanocellulosic materials as bioinks for 3D bioprinting. Biomater Sci 5:1988–1992

    CAS  Google Scholar 

  20. Murphy CA, Collins MN (2016) Microcrystalline cellulose reinforced polylactic acid biocomposite filaments for 3D printing. Polym Compos 15:236–244

    Google Scholar 

  21. Nguyen D, Hägg DA, Forsman A et al (2017) Cartilage tissue engineering by the 3D bioprinting of iPS cells in a nanocellulose/alginate bioink. Sci Reports 7(1):658

    Google Scholar 

  22. Markstedt K, Mantas A, Tournier I et al (2015) 3D bioprinting human chondrocytes with nanocellulose alginate bioink for cartilage tissue engineering applications. Biomacromol 16(5):1489–1496

    CAS  Google Scholar 

  23. Ávila HM, Schwarz S, Rotter N et al (2016) 3D bioprinting of human chondrocyte-laden nanocellulose hydrogels for patient-specific auricular cartilage regeneration. Bioprinting 1(2):22–35

    Google Scholar 

  24. Müller M, Öztürk E, Arlov O et al (2016) Alginate sulfate-nanocellulose bioinks for cartilage bioprinting applications. Ann Biomed Eng 45(1):210–223

    Google Scholar 

  25. Sawkins MJ, Mistry P, Brown BN et al (2015) Cell and protein compatible 3D bioprinting of mechanically strong constructs for bone repair. Biofabrication 7(3):035004

    CAS  Google Scholar 

  26. Torres-Rendon JG, Koepf M, Gehlen D et al (2016) Cellulose nanofibril hydrogel tubes as sacrificial templates for freestanding tubular cell constructs. Biomacromol 17:905–913

    CAS  Google Scholar 

  27. Siqueira G, Kokkinis D, Libanori R et al (2017) Cellulose nanocrystal inks for 3D printing of textured cellular architectures. Adv Funct Mater 27(12):1604619

    Google Scholar 

  28. Lu T, Li Q, Chen W, Yu H (2014) Composite aerogels based on dialdehyde nanocellulose and collagen for potential applications as wound dressing and tissue engineering scaffold. Compos Sci Technol 94(4):132–138

    CAS  Google Scholar 

  29. Jiang Y, Zhou J, Zhang Q et al (2017) Preparation of cellulose nanocrystals from humulus japonicus stem and the influence of high temperature pretreatment. Carbohydr Polym 64:284–293

    Google Scholar 

  30. Iii CMO, Bubnis WA (1996) Chemical and swelling evaluations of amino group crosslinking in gelatin and modified gelatin matrices. Pharm Res 13(12):1821–1827

    Google Scholar 

  31. Zhang R, Ma PX (1999) Poly(alpha-hydroxyl acids)/hydroxyapatite porous composites for bone-tissue engineering I preparation and morphology. J Biomed Mater Res 44(4):446–455

    CAS  Google Scholar 

  32. Chen W, Yu H, Li Q, Liu Y, Li J (2011) Ultralight and highly flexible aerogels with long cellulose nanofibers. Soft Matter 7(21):10360–10368

    CAS  Google Scholar 

  33. Sain M, Panthapulakkal S (2006) Bioprocess preparation of wheat straw fibers and their characterization. Ind Crops Prod 23(1):1–8

    CAS  Google Scholar 

  34. Alemdar A, Sain M (2008) Isolation and characterization of nanofibers from agricultural residues-wheat straw and soy hulls. Biores Technol 99(6):1664–1671

    CAS  Google Scholar 

  35. Münster L, Vícha J, Klofáč J, Masař M, Kucharczyk P, Kuřitka I (2017) Stability and aging of solubilized dialdehyde cellulose. Cellulose 24(7):1–14

    Google Scholar 

  36. Pietrucha K, Safandowska M (2015) Dialdehyde cellulose-crosslinked collagen and its physicochemical properties. Process Biochem 50(12):2105–2111

    CAS  Google Scholar 

  37. Dorris GM, Gray DG (1978) Surface analysis of paper and wood fibres by ESCA II surface composition of mechanical pulps. Cellul Chem Technol 12:721–734

    CAS  Google Scholar 

  38. Gao C, Yan T, Dai K, Wan Y (2012) Immobilization of gelatin onto natural nanofibers for tissue engineering scaffold applications without utilization of any crosslinking agent. Cellulose 19(3):761–768

    CAS  Google Scholar 

  39. Hayami JWS, Waldman SD, Amsden BG (2015) Photo-cross-linked methacrylated polysaccharide solution blends with high chondrocyte viability, minimal swelling, and moduli similar to load bearing soft tissues. Eur Polym J 72:687–697

    CAS  Google Scholar 

  40. Das S, Pati F, Choi YJ et al (2015) Bioprintable, cell-laden silk fibroin-gelatin hydrogel supporting multilineage dif` ferentiation of stem cells for fabrication of three-dimensional tissue constructs. Acta Biomater 11(1):233–246

    CAS  Google Scholar 

  41. Wei LN, Yeong WY, Naing MW (2016) Polyelectrolyte gelatin-chitosan hydrogel optimized for 3d bioprinting in skin tissue engineering. Int J Bioprinting 2(1):53–62

    Google Scholar 

  42. Yashima S, Takase N, Kurokawa T, Gong JP (2014) Friction of hydrogels with controlled surface roughness on solid flat substrates. Soft Matter 10(18):3192–3199

    CAS  Google Scholar 

  43. Kanth SV, Ramaraj A, Rao JR, Nair BU (2015) Stabilization of type I collagen using dialdehyde cellulose. Process Biochem 4:869–874

    Google Scholar 

  44. Huang S, Yao B, Xie J, Fu X (2016) 3d bioprinted extracellular matrix mimics facilitate directed differentiation of epithelial progenitors for sweat gland regeneration. Acta Biomater 32:170–177

    CAS  Google Scholar 

  45. Zhang S, Huang Y, Yang X et al (2009) Gelatin nanofibrous membrane fabricated by electrospinning of aqueous gelatin solution for guided tissue regeneration. J Biomed Mater Res Part A 90(3):671–679

    Google Scholar 

Download references

Acknowledgements

This research was supported by the Excellent Doctoral Thesis Support Program of Yangzhou University, the City and School Cooperation Project, the Top Talents Support Program of Yangzhou University, the Natural Science Foundation of China (81770018).

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Authors and Affiliations

  1. College of Animal Science and Technology, Yangzhou University, No. 48 East Wenhui Road, Yangzhou, Jiangsu, China

    Yani Jiang, Zhe Yang, Xiaodong Xv & Guoqi Zhao

  2. College of Mechanical Engineering, Yangzhou University, No. 196 West Huayang Road, Yangzhou, Jiangsu, China

    Jiping Zhou, Dongfang Liu & Qi Zhang

  3. Medical College, Yangzhou University, No. 11 Huaihai Road, Yangzhou, Jiangsu, China

    Hongcan Shi

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  1. Yani Jiang
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Correspondence to Jiping Zhou.

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Jiang, Y., Zhou, J., Yang, Z. et al. Dialdehyde cellulose nanocrystal/gelatin hydrogel optimized for 3D printing applications. J Mater Sci 53, 11883–11900 (2018). https://doi.org/10.1007/s10853-018-2407-0

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  • DOI: https://doi.org/10.1007/s10853-018-2407-0

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