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Polymer-coated CoFe2O4 nanoassemblies as biocompatible magnetic nanocarriers for anticancer drug delivery

  • Biomaterials
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Abstract

In this work, we report the preparation of cobalt ferrite nanoparticles (CFNPs) coated with hydrophilic polymers guar gum, gum arabic and poly (methacrylic acid) as magnetic nanocarriers and study their conjugation with doxorubicin for the drug release under applied magnetic field. The effect of polymer coating on structural properties is studied using Fourier transform infrared spectroscopy and powder X-ray diffractometry (XRD). The XRD analysis revealed that the polymer coating on the as-synthesized CFNPs has no influence on their crystallite size and it remains between 18 nm and 19 nm. The characteristic morphology, topography and the evidences of polymer coating over the CFNPs are investigated using scanning electron microscopy, atomic force microscopy and thermogravimetric analysis, respectively. Vibrating sample magnetometry revealed the ferromagnetic nature of uncoated CFNPs with a significant saturation magnetization ∼77.2 emu g−1. The dynamic light scattering measurements are also performed to determine the size of uncoated and coated CFNPs. UV–Visible spectroscopy demonstrated a profound loading (70–75%) of doxorubicin onto the magnetic nanocarriers and the efficient release of drug in the presence of external applied magnetic field. In vitro cytotoxic studies confirmed the cytocompatibility mode of coated CFNPs against Chinese Hamster Ovary and Huh-7 cell line, while 0.2 mg mL−1 dose of drug-loaded magnetic nanocarriers inhibited the cell viability of Huh-7 up to 60%. These results strongly encourage the utilization of biocompatible magnetic nanocarriers in targeted drug delivery territory.

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References

  1. Kanagesan S, Aziz SB, Hashim M, Ismail I, Tamilselvan S, Alitheen NB, Swamy MK, Purna Chandra Rao B (2016) Synthesis, characterization and in vitro evaluation of manganese ferrite (MnFe2O4) nanoparticles for their biocompatibility with murine breast cancer cells (4T1). Molecules 21:312

    Article  Google Scholar 

  2. Sheikh A, Jain P (2016) A thorough study of zinc ferrite nanoparticles with reference to green synthesis. Int J Nanomed Nanosurg 2. http://dx.doi.org/10.16966/2470-3206.115

  3. Pardoe H, Clark PR, Pierre TG, Moroz P, Jones SK (2003) A magnetic resonance imaging based method for measurement of tissue iron concentration in liver arterially embolized with ferrimagnetic particles designed for magnetic hyperthermia treatment of tumors. Magn Reson Imaging 21:483–488

    Article  Google Scholar 

  4. Yoza B, Matsumoto M, Matsunaga T (2002) DNA extraction using modified bacterial magnetic particles in the presence of amino silane compound. J Biotechnol 94:217–224

    Article  Google Scholar 

  5. Rudge SR, Kurtz TL, Vessely CR, Catterall LG, Williamson DL (2000) Preparation, characterization, and performance of magnetic iron–carbon composite microparticles for chemotherapy. Biomaterials 21:1411–1420

    Article  Google Scholar 

  6. Das M, Mishra D, Maiti TK, Basak A, Pramanik P (2008) Bio-functionalization of magnetite nanoparticles using an aminophosphonic acid coupling agent: new, ultradispersed, iron-oxide folate nanoconjugates for cancer-specific targeting. Nanotechnology 19:415101

    Article  Google Scholar 

  7. Chauhan RP, Singh G, Singh S et al (2011) Biotinylated magnetic nanoparticles for pretargeting: synthesis and characterization study. Cancer Nanotechnol 2:111–120

    Article  Google Scholar 

  8. Roullin V-G, Deverre J-R, Lemaire L et al (2002) Anti-cancer drug diffusion within living rat brain tissue: an experimental study using [3H](6)-5-fluorouracil-loaded PLGA microspheres. Eur J Pharm Biopharm 53:293–299

    Article  Google Scholar 

  9. Figuerola A, Di Corato R, Manna L, Pellegrino T (2010) From iron oxide nanoparticles towards advanced iron-based inorganic materials designed for biomedical applications. Pharmacol Res 62:126–143

    Article  Google Scholar 

  10. Rahal HT, Awad R, Abdel-Gaber AM, Bakeer DES (2017) Synthesis, characterization, and magnetic properties of pure and EDTA-capped NiO nanosized particles. J Nanomater 2017: 7460323-1

  11. Douziech-Eyrolles L, Marchais H, Hervé K et al (2007) Nanovectors for anticancer agents based on superparamagnetic iron oxide nanoparticles. Int J Nanomed 2:541–550

    Google Scholar 

  12. Yu S-T, Chen T-M, Tseng S-Y, Chen Y-H (2007) Tryptanthrin inhibits MDR1 and reverses doxorubicin resistance in breast cancer cells. Biochem Biophys Res Commun 358:79–84

    Article  Google Scholar 

  13. Alexiou C, Jurgons R, Schmid R, Hilpert A, Bergemann C, Parak F, Iro H (2005) In vitro and in vivo investigations of targeted chemotherapy with magnetic nanoparticles. J Magn Magn Mater 293:389–393

    Article  Google Scholar 

  14. Jurgons R, Seliger C, Hilpert A, Trahms L, Odenbach S, Alexiou C (2006) Drug loaded magnetic nanoparticles for cancer therapy. J Phys Condens Matter 18:S2893

    Article  Google Scholar 

  15. Soares PI, Machado D, Laia C, Pereira LC, Coutinho JT, Ferreira IM, Novo CM, Borges JP (2016) Thermal and magnetic properties of chitosan-iron oxide nanoparticles. Carbohydr Polym 149:382–390

    Article  Google Scholar 

  16. Roy I, Stachowiak MK, Bergey EJ (2008) Nonviral gene transfection nanoparticles: function and applications in the brain. Nanomedicine NBM 4:89–97

    Article  Google Scholar 

  17. Nikalje A (2015) Nanotechnology and its applications in medicine. Med Chem 5:81–89

    Article  Google Scholar 

  18. Chouhan R, Bajpai AK (2009) Real time in vitro studies of doxorubicin release from PHEMA nanoparticles. J Nanobiotechnol 7:5

    Article  Google Scholar 

  19. Maaz K, Mumtaz A, Hasanain SK, Ceylan A (2007) Synthesis and magnetic properties of cobalt ferrite (CoFe2O4) nanoparticles prepared by wet chemical route. J Magn Magn Mater 308:289–295

    Article  Google Scholar 

  20. Tamhankar PM, Kulkarni AM, Watawe SC (2011) Functionalization of cobalt ferrite nanoparticles with alginate coating for biocompatible applications. Mater Sci Appl 02:1317–1321

    Google Scholar 

  21. Rana S, Gallo A, Srivastava RS, Misra RDK (2007) On the suitability of nanocrystalline ferrites as a magnetic carrier for drug delivery: functionalization, conjugation and drug release kinetics. Acta Biomater 3:233–242

    Article  Google Scholar 

  22. Eshraghi M, Kameli P (2011) Magnetic properties of CoFe2O4 nanoparticles prepared by thermal treatment of ball-milled precursors. Curr Appl Phys 11:476–481

    Article  Google Scholar 

  23. Khandekar MS, Kambale RC, Patil JY, Kolekar YD, Suryavanshi SS (2011) Effect of calcination temperature on the structural and electrical properties of cobalt ferrite synthesized by combustion method. J Alloy Compd 509:1861–1865

    Article  Google Scholar 

  24. Ullah Z, Atiq S, Naseem S (2013) Influence of Pb doping on structural, electrical and magnetic properties of Sr-hexaferrites. J Alloys Compd 555:263–267

    Article  Google Scholar 

  25. Salunkhe AB, Khot VM, Thorat ND et al (2013) Polyvinyl alcohol functionalized cobalt ferrite nanoparticles for biomedical applications. Appl Surf Sci 264:598–604

    Article  Google Scholar 

  26. Liu G, Hong RY, Guo L, Li YG, Li HZ (2011) Preparation, characterization and MRI application of carboxymethyl dextran coated magnetic nanoparticles. Appl Surf Sci 257:6711–6717

    Article  Google Scholar 

  27. Sutka A, Mezinskis G, Pludons A, Lagzdina S (2010) Characterization of sol–gel auto-combustion derived spinel ferrite nano-materials. Power Eng 56:254–259

    Google Scholar 

  28. Kooti M, Afshari M (2012) Magnetic cobalt ferrite nanoparticles as an efficient catalyst for oxidation of alkenes. Scientia Iranica 19:1991–1995

    Article  Google Scholar 

  29. Paula IPS, Frederik L, Coro E, Laura CJP, Joana TC, Isabel MMF, Carlos MMN, Joao PMRB (2015) Thermal and magnetic properties of iron oxide colloids: influence of surfactants. Nanotechnology 26:425704

    Article  Google Scholar 

  30. Tummala S, Kumar MNS, Prakash A (2015) Formulation and characterization of 5-Fluorouracil enteric coated nanoparticles for sustained and localized release in treating colorectal cancer. Saudi Pharm J 23:308–314

    Article  Google Scholar 

  31. Lay CL, Liu HQ, Tan HR, Liu Y (2010) Delivery of paclitaxel by physically loading onto poly(ethylene glycol) (PEG)-graftcarbon nanotubes for potent cancer therapeutics. Nanotechnology 21:065101

    Article  Google Scholar 

  32. Soares PI, Sousa AI, Ferreira IM, Novo CM, Borges JP (2016) Towards the development of multifunctional chitosan-based iron oxide nanoparticles: optimization and modelling of doxorubicin release. Carbohydr Polym 153:212–221

    Article  Google Scholar 

  33. Box VGS (2007) The intercalation of DNA double helices with doxorubicin and nagalomycin. J Mol Graph Modell 26:14–19

    Article  Google Scholar 

Download references

Acknowledgements

This research work was supported by Higher Education Commission (HEC), Islamabad, Pakistan.

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Authors and Affiliations

  1. Institute of Chemistry, University of the Punjab, Lahore, 54590, Pakistan

    Muhammad Waheed Mushtaq, Farah Kanwal & Muhammad Zia-ul-Haq

  2. Food Science Department, Rutgers, The State University of the New Jersey, New Brunswick, NJ, 08901, USA

    Muhammad Waheed Mushtaq & Qingrong Huang

  3. Centre of Excellence in Solid State Physics, University of the Punjab, Lahore, 54590, Pakistan

    Aisha Batool & Zaka Ullah

  4. National Center for Nanoscience and Technology, Chinese Academy of Sciences, Beijing, 100190, China

    Aisha Batool

  5. Department of Polymer Engineering and Technology, University of the Punjab, Lahore, 54590, Pakistan

    Tahir Jamil

  6. Applied and Functional Genomics Laboratory, Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, 54590, Pakistan

    Bushra Ijaz

  7. Nanodevice and Material Division, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China

    Zaka Ullah

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  1. Muhammad Waheed Mushtaq
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  2. Farah Kanwal
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Correspondence to Zaka Ullah.

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Mushtaq, M.W., Kanwal, F., Batool, A. et al. Polymer-coated CoFe2O4 nanoassemblies as biocompatible magnetic nanocarriers for anticancer drug delivery. J Mater Sci 52, 9282–9293 (2017). https://doi.org/10.1007/s10853-017-1141-3

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  • DOI: https://doi.org/10.1007/s10853-017-1141-3

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