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SERS tags-based novel monodispersed hollow gold nanospheres for highly sensitive immunoassay of CEA

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Abstract

Here, a highly specific, sensitive, and reliable immunoassay has been developed based on surface-enhanced Raman scattering for quantitative detection of carcinoembryonic antigen (CEA). A novel hollow gold nanospheres (HGNs) was firstly fabricated by seed-mediated methods instead of the one-step methods with high homogeneity and then, the monodispersed HGNs were embedded with 4-mercaptobenzonic acid to form surface-enhanced Raman scattering (SERS) tags and magnetic microspheres were synthesized as enrichment substrates. Based on this sandwich immunoassay strategy, CEA monoclonal antibody -conjugated HGNs-based SERS tags and CEA polyclonal antibodies -conjugated magnetic microspheres were used to detect CEA up to 100 ng/mL with a detection limit of 10 pg/mL, which were two orders of magnitudes lower than that of GNSs-based SERS tags. The monodispersed HGNs were desirable SERS tags for quantitative sandwich immunoassay assay, showing strong potential for the clinical diagnosis of cancer biomarkers.

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Acknowledgements

This work is supported by the Chinese 973 Project (Grant: 2012CB933302), the National Natural Science Foundation of China (Grant: 21175022), the Ministry of Science & Technology of China (Grant: 2012AA022703).

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Authors and Affiliations

  1. State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, People’s Republic of China

    Mingde Guo, Jian Dong, Wei Xie, Lin Tao, Wenbo Lu, Ying Wang & Weiping Qian

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  1. Mingde Guo
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  2. Jian Dong
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  3. Wei Xie
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  4. Lin Tao
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  5. Wenbo Lu
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  6. Ying Wang
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  7. Weiping Qian
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Correspondence to Weiping Qian.

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Guo, M., Dong, J., Xie, W. et al. SERS tags-based novel monodispersed hollow gold nanospheres for highly sensitive immunoassay of CEA. J Mater Sci 50, 3329–3336 (2015). https://doi.org/10.1007/s10853-015-8825-3

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  • DOI: https://doi.org/10.1007/s10853-015-8825-3

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