Skip to main content
SpringerLink
Log in

The impact of Alpha/ESHRE consensus regarding oocytes with aggregates of smooth endoplasmic reticulum (SERa) on in vitro fertilization outcome

  • Assisted Reproduction Technologies
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

The present study aimed to gather information on the impact of Alpha/European Society of Human Reproduction and Embryology (ESHRE) consensus regarding oocytes with aggregates of smooth endoplasmic reticulum (SERa) on in vitro fertilization outcome. In particular, we investigated if patients undergoing intracytoplasmic sperm injection (ICSI) and whose oocytes are discarded due to SERa have a higher chance of embryo transfer cancellation compared to patients without SERa oocytes.

Methods

This is a nested case–control study drawn from the cohort of women referring for in vitro fertilization with ICSI. Cases were patients showing at least one oocyte with SERa at the time of injection. Controls were subsequent patients showing no SERa oocytes and matched ratio 1:1 for age, clinical indication to in vitro fertilization (IVF), and body mass index. The main outcome was the rate of embryo transfer cancellation.

Results

The percentage of women experiencing a transfer cancellation (absence of suitable oocytes or viable embryos) in their ICSI cycle were significantly higher in cases (18 %) compared to controls (8 %) (p = 0.02); however, adjusted odds ratio for FSH and number of SERa oocytes, of follicles, of retrieved oocytes, and of inseminated oocytes were not statistically significant.

Conclusions

We have shown that the exclusion of SERa oocytes from ICSI cycles causes an increased frequency of transfer cancellation. This effect is mostly due to the reduced number of available oocytes after exclusion of SERa oocytes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Vanblerkom J. Occurrence and developmental consequences of aberrant cellular-organization in meiotically mature human oocytes after exogenous ovarian hyperstimulation. J Electron Microsc Tech. 1990;16(4):324–46.

    Article  CAS  Google Scholar 

  2. Serhal PF, Ranieri DM, Kinis A, Marchant S, Davies M, Khadum IM. Oocyte morphology predicts outcome of intracytoplasmic sperm injection. Hum Reprod. 1997;12(6):1267–70.

    Article  CAS  PubMed  Google Scholar 

  3. Rienzi L, Vajta G, Ubaldi F. Predictive value of oocyte morphology in human IVF: a systematic review of the literature. Hum Reprod Update. 2011;17(1):34–45.

    Article  PubMed Central  PubMed  Google Scholar 

  4. Sa R, Cunha M, Silva J, Luis A, Oliveira C, da Silva JT, et al. Ultrastructure of tubular smooth endoplasmic reticulum aggregates in human metaphase II oocytes and clinical implications. Fertil Steril. 2011;96(1):143–9.

    Article  PubMed  Google Scholar 

  5. Hattori H, Nakamura Y, Nakajo Y, Araki Y, Kyono K. Deliveries of babies with normal health derived from oocytes with smooth endoplasmic reticulum clusters. J Assist Reprod Genet. 2014;31(11):1461–7.

    Article  PubMed Central  PubMed  Google Scholar 

  6. Otsuki J, Okada A, Morimoto K, Nagai Y, Kubo H. The relationship between pregnancy outcome and smooth endoplasmic reticulum clusters in MII human oocytes. Hum Reprod. 2004;19(7):1591–7.

    Article  CAS  PubMed  Google Scholar 

  7. Ebner T, Moser M, Shebl O, Sommerguber M, Tews G. Prognosis of oocytes showing aggregation of smooth endoplasmic reticulum. Reprod Biomed Online. 2008;16(1):113–8.

    Article  PubMed  Google Scholar 

  8. Akarsu C, Caglar G, Vicdan K, Sozen E, Biberoglu K. Smooth endoplasmic reticulum aggregations in all retrieved oocytes causing recurrent multiple anomalies: case report. Fertil Steril. 2009;92(4):1496e1–3.

    Article  Google Scholar 

  9. Alpha Scientists in Reproductive Medicine and ESI. The Istanbul consensus workshop on embryo assessment: proceedings of an expert meeting. Hum Reprod. 2011;26(6):1270–83.

    Article  Google Scholar 

  10. Mateizel I, Van Landuyt L, Tournaye H, Verheyen G. Deliveries of normal healthy babies from embryos originating from oocytes showing the presence of smooth endoplasmic reticulum aggregates. Hum Reprod. 2013;28(8):2111–7.

    Article  CAS  PubMed  Google Scholar 

  11. Van Beirs N, Shaw-Jackson C, Rozenberg S, Autin C. Policy of IVF centres towards oocytes affected by Smooth Endoplasmic Reticulum aggregates: a multicentre survey study. J Assist Reprod Genet. 2015;32(6):945–50.

    Article  PubMed  Google Scholar 

  12. Benaglia L, Bermejo A, Somigliana E, Faulisi S, Ragni G, Fedele L, et al. In vitro fertilization outcome in women with unoperated bilateral endometriomas. Fertil Steril. 2013;99(6):1714–9.

    Article  PubMed  Google Scholar 

  13. Kuwayama M. Highly efficient vitrification for cryopreservation of human oocytes and embryos: the Cryotop method. Theriogenology. 2007;67(1):73–80.

    Article  CAS  PubMed  Google Scholar 

  14. Shaw-Jackson C, Van Beirs N, Thomas A-L, Rozenberg S, Autin C. Can healthy babies originate from oocytes with smooth endoplasmic reticulum aggregates? A systematic mini-review. Hum Reprod. 2014;29(7):1380–6.

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Ospedale Maggiore Policlinico—Infertility Unit, Fondazione IRCCS Ca’ Granda, Via Fanti 6, 20122, Milan, Italy

    Liliana Restelli, Silvia Delle Noci, Alice Mangiarini, Stefania Ferrari, Edgardo Somigliana & Alessio Paffoni

Authors
  1. Liliana Restelli
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Silvia Delle Noci
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Alice Mangiarini
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Stefania Ferrari
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Edgardo Somigliana
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Alessio Paffoni
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Alessio Paffoni.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Capsule The exclusion of SERa oocytes from ICSI cycles causes an increased frequency of transfer cancellation.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Restelli, L., Delle Noci, S., Mangiarini, A. et al. The impact of Alpha/ESHRE consensus regarding oocytes with aggregates of smooth endoplasmic reticulum (SERa) on in vitro fertilization outcome. J Assist Reprod Genet 32, 1629–1635 (2015). https://doi.org/10.1007/s10815-015-0583-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10815-015-0583-2

Keywords

Search

Navigation