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Effect of different rehydration temperatures on the survival of human vitrified-warmed oocytes

  • Gamete Biology
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

To evaluate the effect of different exposure temperatures during the dilution process on the survival rate of vitrified oocytes and following development.

Methods

Patients were divided at random into two groups for different dilution temperature (20–22 °C, RT group; 37 °C,37 °C group) according to computer-generated random numbers on the day of oocyte warming. The survival and fertilization rates of vitrified oocytes as well as the implantation and clinical pregnancy rates of the resulting embryos were recorded.

Results

A total of 662 and 676 oocytes were warmed in the 37 °C group and RT group, respectively, and significant difference was observed in the survival rate between 37 °C group (88.37 %) and RT group (79.88 %) (P = 0.0000). There was significant difference between the survival rate of 37 °Cgroup (87.27 %) and RT group (75.64 %) in nondonor patients (P = 0.0001). Multiple linear regression analysis showed that dilution temperature (β = 0.079, P = 0.017) and clinical outcomes of fresh cycles (β = 0.063, P = 0.001) were significantly and independently associated with survival rate. No significant difference was found between the 37 °C group and RT group in: fertilization rate (66.67 versus 65.37 %), implantation rate (20.0 versus19.46 %), clinical pregnancy rate (37.5 versus 35.0 %).

Conclusions

In conclusion, the results of this study give supportive evidence of the application of 37 °C in the dilution process, especially for oocytes of poor quality. Further studies with well-controlled experimental groups are needed to optimize protocols for human oocyte vitrification.

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Acknowledgments

We thank our colleagues in our reproductive center for all of their work toward the study and data collection.

Funding

This study supported by National Basic Research Program of China (973 Program) (2011CB944502; 2012CB944700) and Independent Innovation Funding of Reproductive Medical Hospital Affiliated to Shandong University(20141105).

Conflict of interest

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. Center for Reproductive Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, People’s Republic of China

    Gao Shanshan, Li Mei, Wu Keliang, Sheng Yan, Tang Rong & Chen Zi-Jiang

  2. National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Jinan, People’s Republic of China

    Gao Shanshan, Li Mei, Wu Keliang, Sheng Yan, Tang Rong & Chen Zi-Jiang

  3. Reproductive Medical Hospital Affiliated to Shandong University, Jinan, People’s Republic of China

    Gao Shanshan, Li Mei, Wu Keliang, Sheng Yan, Tang Rong & Chen Zi-Jiang

  4. The Key Laboratory for Reproductive Endocrinology of Ministry of Education, Jinan, People’s Republic of China

    Gao Shanshan, Li Mei, Wu Keliang, Sheng Yan, Tang Rong & Chen Zi-Jiang

  5. Shandong Provincial Key Laboratory of Reproductive Medicine, Jinan, People’s Republic of China

    Gao Shanshan, Li Mei, Wu Keliang, Sheng Yan, Tang Rong & Chen Zi-Jiang

Authors
  1. Gao Shanshan
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  2. Li Mei
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  3. Wu Keliang
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  4. Sheng Yan
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  5. Tang Rong
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  6. Chen Zi-Jiang
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Corresponding author

Correspondence to Chen Zi-Jiang.

Additional information

Capsule

The temperature at which the oocytes are exposed to CPA solutions might have different osmotic and toxic consequences on cryosurvival and subsequent development. In this study, we aimed to compare the effect of different temperatures during rehydration on the cryosurvival and subsequent development of vitrified oocytes from infertile patients. The results of this study give supportive evidence of the application of 37 °C in the dilution process, especially for oocytes of poor quality.

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Shanshan, G., Mei, L., Keliang, W. et al. Effect of different rehydration temperatures on the survival of human vitrified-warmed oocytes. J Assist Reprod Genet 32, 1197–1203 (2015). https://doi.org/10.1007/s10815-015-0480-8

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  • DOI: https://doi.org/10.1007/s10815-015-0480-8

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