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Association between a PD-1 gene polymorphism and antisperm antibody-related infertility in Iranian men

  • Genetics
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Objective

Programmed cell death-1 (PD-1, Pdcd1), an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signalling by recruiting protein tyrosine phosphatase, SHP-2 upon interacting with either of two ligands, PD-L1 or PD-L2. This study investigates PD-1 gene polymorphism in patients with antisperm antibody-related infertility

Methods

Genotyping was performed by polymerase chain reaction and restriction enzyme digestion (PCR-RFLP), this polymorphism was genotyped in 145 Iranian subjects (61 patients with antisperm antibody-related infertility and 84 healthy controls).

Results

Patients frequencies of the G/A genotype in comparison with healthy controls (38.2 % vs. 32.7 %, OR =1.21, P = 0.35) were not significantly different. However, G/G and A/A genotype frequencies between patients and healthy controls were significantly different (P = 0.042, P = 0.00001, respectively). Also, allele frequencies of this polymorphism were significantly different (P = 0.0012) in patients compared to healthy controls.

Conclusion

According to these results, there is a correlation between PD-1 gene polymorphism and susceptibility to antisperm antibody-related infertility in our study group.

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Acknowledgments

We are grateful to the ASA-related patients and the healthy blood donors who provided blood samples for this research.

Author information

Authors and Affiliations

  1. Department of Immunology and Biology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Mohammad Reza Zamani, Ahmad Massoud & Nima Rezaei

  2. Department of Obstetrics and gynaecology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Firouzeh Akbari Asbagh

  3. Department of Microbiology & Immunology, Harvard Medical School, MA, Boston, USA

    Amir Hossein Massoud

  4. Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Arash Salmaninejad

  5. Research Center for Immunodeficiencies, Children’s Medical Center, Tehran University of Medical Sciences, Tehran, Iran

    Nima Rezaei

Authors
  1. Mohammad Reza Zamani
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  2. Firouzeh Akbari Asbagh
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  3. Amir Hossein Massoud
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  4. Arash Salmaninejad
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  5. Ahmad Massoud
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  6. Nima Rezaei
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Corresponding author

Correspondence to Nima Rezaei.

Additional information

Capsule Anti-sperm antibodies (ASA) production is a result of an abnormal immune condition and can cause infertility in men. We found a significant correlation between PD-1.3 genotypes and risk of ASA-related infertility. These findings raises interests for further investigation to the mechanism of PD-1/PD-1 ligand interaction in the development of ASA.

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Zamani, M.R., Asbagh, F.A., Massoud, A.H. et al. Association between a PD-1 gene polymorphism and antisperm antibody-related infertility in Iranian men. J Assist Reprod Genet 32, 103–106 (2015). https://doi.org/10.1007/s10815-014-0371-4

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  • DOI: https://doi.org/10.1007/s10815-014-0371-4

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