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Association of IL-17A and IL-17 F gene polymorphisms with recurrent pregnancy loss in Iranian women

  • Genetics
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

Recurrent pregnancy loss (RPL) is defined as the occurrence of two or more miscarriages before the 20th week of pregnancy. T helper17 cells are a novel subset of T cells, which secrete IL (Interleukin)-17 and are known to be involved in inflammation, autoimmunity and rejection of non-self tissues. Herein, we studied the association between IL-17A rs2275913 and IL-17F rs763780 gene polymorphisms with RPL in Iranian women.

Methods

A case-controlled study was performed on two groups consisting of 85 healthy women with at least one delivery and 85 women with the history of two or more RPLs. The frequency of IL-17A rs2275913 and IL-17 F rs763780 polymorphisms were determined by PCR-RFLP.

Results

In the RPL group, the genotypes frequencies of rs2275913 polymorphism were GG (8.2 %), AG (30.6 %), and AA (61.2 %) and in the control group, were GG (3.5 %), AG (42.4 %) and AA (54.1 %). Statistical analysis showed no significant difference between the genotypes of AA, AG and GG in the two groups (p = 0.1). The genotypes frequencies of rs763780 polymorphism were TT (43.5 %), TC (49.4 %) and CC (7.1 %) in the RPL group; whereas the frequencies were TT (25.9 %), TC (70.6 %) and CC (3.5 %) in the control group. Statistical analysis revealed a significant difference in the TT, TC, and CC genotypes frequencies between the case and the control groups (p = 0.01).

Conclusions

Our findings indicate that IL-17F polymorphism, rs763780, might be associated with a high risk of RPL in Iranian women.

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Acknowledgments

Shahid Sadoughi University of Medical Sciences provided the grant for this research project. This manuscript is a part of in the M.Sc thesis of Soheil Najafi. The authors are grateful to Drs. Farshid Noorbakhsh and Kazem Barzegar for their critical reading of the manuscript and Dr. Hossein Fallahzadeh for statistical assistance.

Conflicts of Interest

The authors declare that they have no conflicts of interest.

Author information

Authors and Affiliations

  1. Department of Immunology, Reproductive Immunology Research Center, Shahid Sadoughi University of Medical Sciences, Daneshjou Blv, Pirapezeshki, Yazd, Iran

    Soheil Najafi & Hossein Hadinedoushan

  2. Department of Parasitology and Mycology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

    Gilda Eslami

  3. Department of Gynecology and Obstetrics, Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

    Abbas Aflatoonian

Authors
  1. Soheil Najafi
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  2. Hossein Hadinedoushan
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  3. Gilda Eslami
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  4. Abbas Aflatoonian
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Corresponding author

Correspondence to Hossein Hadinedoushan.

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Capsule

The role of IL-17 in the pathogenesis of the recurrent pregnancy loss (RPL) has remained unclear. This is first report frequency of IL-17 gene polymorphism in RPL subjects and controls in Iranian population. IL-17 F polymorphism, rs763780, might be associated with a high risk of RPL.

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Najafi, S., Hadinedoushan, H., Eslami, G. et al. Association of IL-17A and IL-17 F gene polymorphisms with recurrent pregnancy loss in Iranian women. J Assist Reprod Genet 31, 1491–1496 (2014). https://doi.org/10.1007/s10815-014-0294-0

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  • DOI: https://doi.org/10.1007/s10815-014-0294-0

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