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Age-associated changes in bovine oocytes and granulosa cell complexes collected from early antral follicles

  • Gamete Biology
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

To assess the age-associated changes in oocytes and granulosa cells derived from early antral follicles (EAFs).

Method

Gene expression analysis of granulosa cells of the EAFs using a genome analyzer (Illumina) and in vitro culture of oocyte-granulosa cell complexes (OGCs) of EAFs (400–700 μm in diameter) collected from ovaries of aged (>120 months) and young (<50 months) cows.

Results

Gene expression profiles in granulosa cells of EAFs of aged cows, which included changes in genes that encode chaperone proteins and antioxidants. In vivo development of EAFs, as determined by oocyte diameter of EAFs and AFs (3–6 mm in diameter), appeared to be impaired in aged cows and the OGCs of aged cows contained low GSH compared to younger counterparts. When the OGCs were cultured in a medium containing low estradiol (E2, 0.1 μg/mL), the ratio of antrum formation was higher for OGCs from aged animals than that from young animals, while higher abnormal fertilization rate and lower total cell number of the blastocysts were observed in the OGCs of aged cows compared with those of young cows. On the contrary, when the OGCs were cultured in a medium containing 10 μg/mL E2, the ratio of antrum formation and fertilization outcome was comparable between the two age groups, whereas the total cell number of the blastocysts was still low in the aged group.

Conclusion

Aging affects the gene expression profiles of the granulosa cells, and impairs in vitro developmental ability of OGCs collected from EAFs.

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Acknowledgments

We thank Yuh Shiwa, Misaki Imai, Hikaru Wada, Chihiro Yamamoto, and Kazuma Tsunematsu for technical support.　This study was supported by the Promotion and Mutual Aid Corporation for Private Schools of Japan and the Ministry of Education, Culture, Sports, Science, and Technology [Grants-in-Aid for Scientific Research (S0801025)] and Grant-in-Aid for Scientific Research C (KAKENHI, grant number: 25450400) from the Japan society for the Promotion of Science.

Author information

Authors and Affiliations

  1. Department of Animal Science, Tokyo University of Agriculture, Atugi, 243-0034, Japan

    N. Itami, H. Kawana, M. Endo, T. Kuwayama & H. Iwata

  2. NODAI Research Institute Genome Research Center, Tokyo University of Agriculture, Setagaya, 156-8502, Japan

    R. Kawahara-Miki

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  1. N. Itami
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  2. R. Kawahara-Miki
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  3. H. Kawana
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Corresponding author

Correspondence to H. Iwata.

Additional information

Capsule N Itami conducted culture experiment and wrote this paper and R Kawahara-Miki conducted gene expression analysis and wrote this paper. Their contributions are equal.

N Itami conducted culture experiments and R Kawahara-Miki conducted gene expression analysis and both authors contributed equally to this study.

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Itami, N., Kawahara-Miki, R., Kawana, H. et al. Age-associated changes in bovine oocytes and granulosa cell complexes collected from early antral follicles. J Assist Reprod Genet 31, 1079–1088 (2014). https://doi.org/10.1007/s10815-014-0251-y

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