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Preimplantation Genetic Screening (PGS) with Comparative Genomic Hybridization (CGH) following day 3 single cell blastomere biopsy markedly improves IVF outcomes while lowering multiple pregnancies and miscarriages

  • Assisted Reproduction Technologies
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Abstract

Purpose

To determine benefits of cleavage-stage preimplantation genetic screening (PGS) by array comparative genomic hybridization (CGH).

Methods

A retrospective case–control study was performed at a tertiary care university-affiliated medical center. Implantation rate was looked at as a primary outcome. Secondary outcomes included clinical and ongoing pregnancy rates, as well as multiple pregnancy and miscarriage rates. Thirty five patients underwent 39 fresh cycles with PGS by aCGH and 311 similar patients underwent 394 invitro fertilization cycles.

Result(s)

The implantation rate in the CGH group doubled when compared to the control group (52.63 % vs. 19.15 %, p = <0.001), clinical pregnancy rate was higher (69.23 % vs. 43.91 %, p = 0.0002), ongoing pregnancy rate almost doubled (61.54 % vs. 32.49 %, p = <0.0001), multiple pregnancy rate decreased (8.33 % vs. 34.38 %, p = 0.0082) and miscarriage rate trended lower (11.11 % vs. 26.01 %, p = 0.13).

Conclusion

Cleavage stage PGS with CGH is a feasible and safe option for aneuploidy screening that shows excellent outcomes when used in fresh cycles. This is the first report of cleavage stage PGS by CGH showing improved ongoing pregnancy rates.

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Acknowledgments

The authors thank the office and embryology staff of the Continuum Reproductive Center, and wish to acknowledge the assistance of Carolyn Waldron, MS, MA.

Disclosure

The authors have nothing to disclose.

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Authors and Affiliations

  1. Continuum Reproductive Center, Department of Obstetrics and Gynecology, St. Luke’s-Roosevelt Hospital Center, Columbia University, College of Physicians and Surgeons, New York, NY, USA

    Martin D. Keltz, Mario Vega, Ido Sirota, Matthew Lederman, Eric Gonzales & Daniel Stein

  2. Department of Community and Preventive Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA

    Erin L. Moshier

  3. Director Division of Reproductive Endocrinology, The Continuum Reproductive Center, St. Luke’s-Roosevelt Hospital Center, 425 West 59th Street Ste. 5A, New York, NY, 10019, USA

    Martin D. Keltz

Authors
  1. Martin D. Keltz
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  2. Mario Vega
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  3. Ido Sirota
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  4. Matthew Lederman
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  7. Daniel Stein
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Corresponding author

Correspondence to Martin D. Keltz.

Additional information

Martin D. Keltz and Mario Vega contributed equally to this article.

Capsule Cleavage-stage PGS with CGH results in higher implantation, clinical pregnancy and ongoing pregnancy rates, while lowering multiple pregnancy and miscarriage rates.

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Keltz, M.D., Vega, M., Sirota, I. et al. Preimplantation Genetic Screening (PGS) with Comparative Genomic Hybridization (CGH) following day 3 single cell blastomere biopsy markedly improves IVF outcomes while lowering multiple pregnancies and miscarriages. J Assist Reprod Genet 30, 1333–1339 (2013). https://doi.org/10.1007/s10815-013-0070-6

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  • DOI: https://doi.org/10.1007/s10815-013-0070-6

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