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Establishment of mouse embryonic stem cells from isolated blastomeres and whole embryos using three derivation methods

  • Stem Cell Biology
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Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

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Abstract

Purpose

The aim of the present study is to compare three previously described mouse embryonic stem cell derivation methods to evaluate the influence of culture conditions, number of isolated blastomeres and embryonic stage in the derivation process.

Methods

Three embryonic stem cell derivation methods: standard, pre-adhesion and defined culture medium method, were compared in the derivation from isolated blastomeres and whole embryos at 4- and 8-cell stages.

Results

A total of 200 embryonic stem cell lines were obtained with an efficiency ranging from 1.9% to 72%.

Conclusions

Using either isolated blastomeres or whole embryos, the highest rates of mouse embryonic stem cell establishment were achieved with the defined culture medium method and efficiencies increased as development progressed. Using isolated blastomeres, efficiencies increased in parallel to the proportion of the embryo volume used to start the derivation process.

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Acknowledgements

This work received financial support from the Spanish Ministerio de Educación y Ciencia (MEC) projects BIO 2005-04341 and BIO2006-11792, the Generalitat de Catalunya DGR project #2005SGR-0047 and MEC FPU fellowship AP2006-02038. We thank Marc Puigcerver and Jonatan Lucas their technical assistance.

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Authors and Affiliations

  1. Departament de Biologia Cel·lular, Fisiologia i Immunologia, Facultat de Biociències, Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain

    Sheyla González, Elena Ibáñez & Josep Santaló

Authors
  1. Sheyla González
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  2. Elena Ibáñez
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  3. Josep Santaló
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Correspondence to Josep Santaló.

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Capsule

Influence of the embryonic stage and the number of isolated blastomeres on the derivation of mouse embryonic stem cells using three described derivation methods.

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González, S., Ibáñez, E. & Santaló, J. Establishment of mouse embryonic stem cells from isolated blastomeres and whole embryos using three derivation methods. J Assist Reprod Genet 27, 671–682 (2010). https://doi.org/10.1007/s10815-010-9473-9

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  • DOI: https://doi.org/10.1007/s10815-010-9473-9

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