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Slow and ultrarapid cryopreservation of biopsied mouse blastocysts and its effect on DNA integrity index

  • Fertility Preservation
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Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

To evaluate the effect of slow and ultra-rapid freezing on biopsied blastocysts’ DNA integrity.

Methods

Forty eight mouse blastocysts were biopsied of which 16 were cryopreserved by slowly freezing and 17 by vitrification. Fourteen intact blastocysts were slowly cryopreserved and 24 were vitrified. Eighteen fresh intact blastocysts and fifteen biopsied blastocysts served as controls. The DNA integrity index of all blastocysts was evaluated using (TUNEL) staining and confocal imaging

Results

Both slow freezing and vitrification of biopsied blastocysts induced apoptosis to a similar extent. Biopsying blastocysts before vitrification resulted in less apoptosis than vitrification of intact blastocysts.

Conclusion

Slow freezing and vitrification are equal options for preservation of biopsied blastocysts as regards the DNA integrity index (DII). Biopsied blastocysts better tolerate vitrification than intact expanded blastocysts.

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Acknowledgement

We would like to acknowledge Dr Hussein Abdelrazik for his assistance in the biopsy procedures.

Author information

Authors and Affiliations

  1. Center For Reproductive Medicine, Glickman Urological & Kidney Institute and Obstetrics and Gynecology and Women’s Health Institute, Cleveland Clinic, 9500 Euclid Avenue, Desk A19.1, Cleveland, OH, 44195, USA

    Amr Kader, Tommaso Falcone, Rakesh K. Sharma, Devna Mangrola & Ashok Agarwal

  2. Department of Obstetrics and Gynecology, West Virginia University, Morgantown, WV, USA

    Amr Kader

  3. Department of Obstetrics and Gynecology, Alexandria University, Alexandria, Egypt

    Amr Kader

Authors
  1. Amr Kader
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  2. Tommaso Falcone
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  3. Rakesh K. Sharma
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  4. Devna Mangrola
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  5. Ashok Agarwal
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Corresponding author

Correspondence to Ashok Agarwal.

Additional information

Presented at the 64th Annual Meeting of the American Society for Reproductive Medicine, San Francisco, CA, 2008

Capsule

Biopsied blastocysts are negatively affected by slow freezing and vitrification to the same extent. Biopsied blastocysts can better tolerate vitrification than intact blastocysts as regards their DNA integrity.

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Kader, A., Falcone, T., Sharma, R.K. et al. Slow and ultrarapid cryopreservation of biopsied mouse blastocysts and its effect on DNA integrity index. J Assist Reprod Genet 27, 509–515 (2010). https://doi.org/10.1007/s10815-010-9441-4

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  • DOI: https://doi.org/10.1007/s10815-010-9441-4

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