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Are zona pellucida laser drilling and polar body biopsy safe for in vitro matured oocytes?

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Abstract

Introduction

Preconception diagnosis requires first polar body biopsy. When the hole in the zona pellucida is made with a laser beam, heat propagation could, like the biopsy itself, be deleterious. Our aim was to evaluate the effect of this technique on human in vitro matured oocyte and embryo development.

Methods

One hunded fifty five retrieved immature oocytes from 75 women, matured in vitro, were distributed in 3 groups: 50 oocytes in a control group, without laser drilling and first polar body biopsy, 52 oocytes in a group with only laser drilling, and 53 oocytes in a group with both laser drilling and first polar body biopsy. Safety was evaluated using four criteria: [1] oocyte lysis rate, [2] oocyte activation rate, [3] oocyte development after calcium ionophore treatment, [4] and embryo chromosome breakage incidence after Tarkowski preparation.

Results

No difference in the four criteria was observed between the 3 oocyte groups.

Conclusions

We did not find evidence of deleterious effect of laser drilling and first polar body biopsy on in vitro matured oocytes, according to our criteria.

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References

  1. Pellestor F. Frequency and distribution of aneuploidy in human female gametes. Hum Genet. 1991;86(3):283–8.

    Article  CAS  PubMed  Google Scholar 

  2. Hassold T, Hunt P. To err (meiotically) is human: the genesis of human aneuploidy. Nat Rev Genet. 2001;2(4):280–91.

    Article  CAS  PubMed  Google Scholar 

  3. Hassold T, Abruzzo M, Adkins K, et al. Human aneuploidy: incidence, origin, and etiology. Environ Mol Mutagen. 1996;28(3):167–75.

    Article  CAS  PubMed  Google Scholar 

  4. Pellestor F, Andreo B, Arnal F, et al. Maternal aging and chromosomal abnormalities: new data drawn from in vitro unfertilized human oocytes. Hum Genet. 2003;112(2):195–203.

    PubMed  Google Scholar 

  5. Vialard F, Petit C, Bergere M, et al. Evidence of a high proportion of premature unbalanced separation of sister chromatids in the first polar bodies of women of advanced age. Hum Reprod. 2006;21(5):1172–8.

    Article  CAS  PubMed  Google Scholar 

  6. Gianaroli L, Magli MC, Ferraretti AP, et al. The role of preimplantation diagnosis for aneuploidies. Reprod Biomed Online. 2002;4 Suppl 3:31–6.

    PubMed  Google Scholar 

  7. Montag M, van der Ven K, Dorn C, van der Ven H. Outcome of laser-assisted polar body biopsy and aneuploidy testing. Reprod Biomed Online. 2004;9(4):425–9.

    Article  PubMed  Google Scholar 

  8. Verlinsky Y, Tur-Kaspa I, Cieslak J, et al. Preimplantation testing for chromosomal disorders improves reproductive outcome of poor-prognosis patients. Reprod Biomed Online. 2005;11(2):219–25.

    Article  CAS  PubMed  Google Scholar 

  9. Findikli N, Kahraman S, Saglam Y, et al. Embryo aneuploidy screening for repeated implantation failure and unexplained recurrent miscarriage. Reprod Biomed Online. 2006;13(1):38–46.

    Article  CAS  PubMed  Google Scholar 

  10. Gianaroli L, Magli MC, Ferraretti AP, et al. The beneficial effects of preimplantation genetic diagnosis for aneuploidy support extensive clinical application. Reprod Biomed Online. 2005;10(5):633–40.

    Article  PubMed  Google Scholar 

  11. Gianaroli L, Magli MC, Ferraretti AP, Munne S. Preimplantation diagnosis for aneuploidies in patients undergoing in vitro fertilization with a poor prognosis: identification of the categories for which it should be proposed. Fertil Steril. 1999;72(5):837–44.

    Article  CAS  PubMed  Google Scholar 

  12. Verlinsky Y, Cohen J, Munne S, et al. Over a decade of experience with preimplantation genetic diagnosis: a multicenter report. Fertil Steril. 2004;82(2):292–4.

    Article  PubMed  Google Scholar 

  13. Kuliev A, Cieslak J, Ilkevitch Y, Verlinsky Y. Chromosomal abnormalities in a series of 6, 733 human oocytes in preimplantation diagnosis for age-related aneuploidies. Reprod Biomed Online. 2003;6(1):54–9.

    Article  PubMed  Google Scholar 

  14. Staessen C, Platteau P, Van Assche E, et al. Comparison of blastocyst transfer with or without preimplantation genetic diagnosis for aneuploidy screening in couples with advanced maternal age: a prospective randomized controlled trial. Hum Reprod. 2004;19(12):2849–58.

    Article  PubMed  Google Scholar 

  15. Mastenbroek S, Twisk M, van Echten-Arends J, et al. In vitro fertilization with preimplantation genetic screening. N Engl J Med. 2007;357(1):9–17.

    Article  CAS  PubMed  Google Scholar 

  16. Hardarson T, Hanson C, Lundin K, et al. Preimplantation genetic screening in women of advanced maternal age caused a decrease in clinical pregnancy rate: a randomized controlled trial. Hum Reprod. 2008;23(12):2806–12.

    Article  CAS  PubMed  Google Scholar 

  17. Blockeel C, Schutyser V, De Vos A, et al. Prospectively randomized controlled trial of PGS in IVF/ICSI patients with poor implantation. Reprod Biomed Online. 2008;17(6):848–54.

    Article  PubMed  Google Scholar 

  18. Munne S, Cohen J, Simpson JL. In vitro fertilization with preimplantation genetic screening. N Engl J Med. 2007;357(17):1769–70. author reply 1770-1.

    Article  CAS  PubMed  Google Scholar 

  19. Wilton LJ. In vitro fertilization with preimplantation genetic screening. N Engl J Med. 2007;357(17):1770. author reply 1770-1.

    CAS  PubMed  Google Scholar 

  20. Handyside AH, Thornhill AR. In vitro fertilization with preimplantation genetic screening. N Engl J Med. 2007;357(17):1770. author reply 1770-1.

    CAS  PubMed  Google Scholar 

  21. Twisk M, Mastenbroek S, van Wely M, et al. Preimplantation genetic screening for abnormal number of chromosomes (aneuploidies) in in vitro fertilisation or intracytoplasmic sperm injection. Cochrane Database Syst Rev. 2006;1:CD005291.

    PubMed  Google Scholar 

  22. Cohen J, Grifo JA. Multicentre trial of preimplantation genetic screening reported in the New England Journal of Medicine: an in-depth look at the findings. Reprod Biomed Online. 2007;15(4):365–6.

    Article  PubMed  Google Scholar 

  23. Simpson JL. What next for preimplantation genetic screening? Randomized clinical trial in assessing PGS: necessary but not sufficient. Hum Reprod. 2008;23(10):2179–81.

    Article  PubMed  Google Scholar 

  24. Harper J, Sermon K, Geraedts J, et al. What next for preimplantation genetic screening? Hum Reprod. 2008;23(3):478–80.

    Article  PubMed  Google Scholar 

  25. Montag M, van der Ven K, Rosing B, van der Ven H. Polar body biopsy: a viable alternative to preimplantation genetic diagnosis and screening. Reprod Biomed Online. 2009;18 Suppl 1:6–11.

    Article  PubMed  Google Scholar 

  26. Verlinsky Y, Ginsberg N, Lifchez A, et al. Analysis of the first polar body: preconception genetic diagnosis. Hum Reprod. 1990;5(7):826–9.

    CAS  PubMed  Google Scholar 

  27. Handyside AH, Kontogianni EH, Hardy K, Winston RM. Pregnancies from biopsied human preimplantation embryos sexed by Y-specific DNA amplification. Nature. 1990;344(6268):768–70.

    Article  CAS  PubMed  Google Scholar 

  28. Boada M, Carrera M, De La Iglesia C, et al. Successful use of a laser for human embryo biopsy in preimplantation genetic diagnosis: report of two cases. J Assist Reprod Genet. 1998;15(5):302–7.

    Article  CAS  PubMed  Google Scholar 

  29. Palanker D, Ohad S, Lewis A, et al. Technique for cellular microsurgery using the 193-nm excimer laser. Lasers Surg Med. 1991;11(6):580–6.

    Article  CAS  PubMed  Google Scholar 

  30. Germond M, Nocera D, Senn A, et al. Microdissection of mouse and human zona pellucida using a 1.48-microns diode laser beam: efficacy and safety of the procedure. Fertil Steril. 1995;64(3):604–11.

    CAS  PubMed  Google Scholar 

  31. Chatzimeletiou K, Picton HM, Handyside AH. Use of a non-contact, infrared laser for zona drilling of mouse embryos: assessment of immediate effects on blastomere viability. Reprod Biomed Online. 2001;2(3):178–87.

    PubMed  Google Scholar 

  32. Montag M, van der Ven K, Delacretaz G, et al. Laser-assisted microdissection of the zona pellucida facilitates polar body biopsy. Fertil Steril. 1998;69(3):539–42.

    Article  CAS  PubMed  Google Scholar 

  33. Chatzimeletiou K, Morrison EE, Panagiotidis Y, et al. Comparison of effects of zona drilling by non-contact infrared laser or acid Tyrode's on the development of human biopsied embryos as revealed by blastomere viability, cytoskeletal analysis and molecular cytogenetics. Reprod Biomed Online. 2005;11(6):697–710.

    Article  PubMed  Google Scholar 

  34. Tesarik J, Mendoza C, Greco E. The activity (calcium oscillator?) responsible for human oocyte activation after injection with round spermatids is associated with spermatid nuclei. Fertil Steril. 2000;74(6):1245–7.

    Article  CAS  PubMed  Google Scholar 

  35. Tesarik J, Testart J. Treatment of sperm-injected human oocytes with Ca2+ ionophore supports the development of Ca2+ oscillations. Biol Reprod. 1994;51(3):385–91.

    Article  CAS  PubMed  Google Scholar 

  36. Tarkowski AK. An air-drying method for chromosome preparations from mouse eggs. Cytogenetics. 1966;5:394–400.

    Article  Google Scholar 

  37. Verlinsky Y, Pergament E, Strom C. The preimplantation genetic diagnosis of genetic diseases. J In Vitro Fert Embryo Transf. 1990;7(1):1–5.

    Article  CAS  PubMed  Google Scholar 

  38. Kuliev A, Cieslak J, Verlinsky Y. Frequency and distribution of chromosome abnormalities in human oocytes. Cytogenet Genome Res. 2005;111(3-4):193–8.

    Article  CAS  PubMed  Google Scholar 

  39. Gordon JW, Grunfeld L, Garrisi GJ, et al. Fertilization of human oocytes by sperm from infertile males after zona pellucida drilling. Fertil Steril. 1988;50(1):68–73.

    CAS  PubMed  Google Scholar 

  40. Magli MC, Gianaroli L, Ferraretti AP, et al. The combination of polar body and embryo biopsy does not affect embryo viability. Hum Reprod. 2004;19(5):1163–9.

    Article  PubMed  Google Scholar 

  41. Clement-Sengewald A, Buchholz T, Schutze K. Laser microdissection as a new approach to prefertilization genetic diagnosis. Pathobiology. 2000;68(4-5):232–6.

    Article  CAS  PubMed  Google Scholar 

  42. (1998) Assisted reproduction by intracytoplasmic sperm injection: a survey on the clinical experience in 1994 and the children born after ICSI, carried out until 31 December 1993. ESHRE Task Force on Intracytoplasmic Sperm Injection. European Society for Human Reproduction and Embryology. Hum Reprod. 13(6): p. 1737–46.

  43. http://perso.wanadoo.fr/fivnat.fr/).

  44. Douglas-Hamilton DH, Conia J. Thermal effects in laser-assisted pre-embryo zona drilling. J Biomed Opt. 2001;6(2):205–13.

    Article  CAS  PubMed  Google Scholar 

  45. Pellestor F, Andreo B, Arnal F, et al. Mechanisms of non-disjunction in human female meiosis: the co-existence of two modes of malsegregation evidenced by the karyotyping of 1397 in-vitro unfertilized oocytes. Hum Reprod. 2002;17(8):2134–45.

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Reproductive Biology and Cytogenetics and Gynaecology, CHI Poissy St Germain, Poissy, Cedex, 78303, France

    Ibrahim Hammoud, Denise Molina-Gomes, Martine Albert, Marianne Bergere, Marc Bailly, Robert Wainer, Jacqueline Selva & Francois Vialard

  2. EA2493, UVSQ, Versailles, France

    Ibrahim Hammoud, Martine Albert, Marianne Bergere, Jacqueline Selva & Francois Vialard

Authors
  1. Ibrahim Hammoud
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  2. Denise Molina-Gomes
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  3. Martine Albert
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  4. Marianne Bergere
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  5. Marc Bailly
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  6. Robert Wainer
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  7. Jacqueline Selva
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  8. Francois Vialard
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Corresponding author

Correspondence to Francois Vialard.

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Capsule Safety evaluation of zona pellucida laser drilling and polar body biopsy on in vitro matured oocytes: lysis rate, activation rate and oocyte development.

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Hammoud, I., Molina-Gomes, D., Albert, M. et al. Are zona pellucida laser drilling and polar body biopsy safe for in vitro matured oocytes?. J Assist Reprod Genet 27, 423–427 (2010). https://doi.org/10.1007/s10815-010-9422-7

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  • DOI: https://doi.org/10.1007/s10815-010-9422-7

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