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Male infertility in China: laboratory finding for AZF microdeletions and chromosomal abnormalities in infertile men from Northeastern China

  • Genetics
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purposes

To investigate the frequencies of AZF microdeletions and chromosomal abnormalities in infertile men from Northeastern China. Moreover, to compare the prevalence of these abnormalities with other countries and regions in the world.

Methods

305 infertile men were enrolled. A complete semen analysis and reproductive hormones were measured according to standard methods. Multiplex polymerase chain reaction (PCR) amplification using nine specific sequence-tagged sites (STS) were used to detect AZF microdeletions. Karyotype analyses were performed on peripheral blood lymphocytes with standard G-banding.

Results

Of the 305 infertile men, 28 (9.2%) had AZF microdeletions and 26 (8.5%) had chromosomal abnormalities. The most frequent microdeletions were in the AZFc+d, followed by AZFc, AZFb+c+d and AZFa. A total of 19 patients (82.6%) had Klinefelter′s syndrome (47, XXY) in the azoospermic group.

Conclusions

The freqencies of AZF microdeletions and chromosomal abnormalities in infertile men from Northeastern China were comparable with infertile men from other countries and regions. However, there was a slightly higher prevalence rate of AZF microdeletions in oligozoospermic patients than reported in previous studies.

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Acknowledgements

We thank all the patients and donors of DNA samples. We are grateful to all staff of the Andrology Laboratory for their excellent work. We also thank Professor Frederick William Orr for his English-language assistance and critical review. This work was kindly supported by the funds of the Office of Science and Technology project in Jilin Province (NO. 200705371).

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Authors and Affiliations

  1. Center for Reproductive Medicine, First Hospital, Jilin University, Changchun, 130021, China

    Rui-Xue Wang, Chao Fu, Ya-Ping Yang, Rong-Rong Han, Yuan Dong, Ru-Lin Dai & Rui-Zhi Liu

  2. Department of Cell Biology, Norman Bethune Medical School, Jilin University, Changchun, 130021, China

    Rui-Zhi Liu

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  1. Rui-Xue Wang
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  2. Chao Fu
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Corresponding author

Correspondence to Rui-Zhi Liu.

Additional information

Capsule It is essential for infertile men from Northeastern China to take genetic counseling and reliable screening for AZF microdeletions and chromosomal abnormalities.

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Wang, RX., Fu, C., Yang, YP. et al. Male infertility in China: laboratory finding for AZF microdeletions and chromosomal abnormalities in infertile men from Northeastern China. J Assist Reprod Genet 27, 391–396 (2010). https://doi.org/10.1007/s10815-010-9420-9

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  • DOI: https://doi.org/10.1007/s10815-010-9420-9

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