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Closed-system solid surface vitrification versus slow programmable freezing of mouse 2-cell embryos

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Abstract

Purpose

To compare closed-system solid surface vitrification with slow freezing.

Methods

Mouse 2-cell embryos (n = 348) were divided into vitrification, slow freezing and non-frozen groups. For vitrification, embryos were exposed to 10% ethylene glycol (EG), 10% dimethylsulfoxide (DMSO) and 10% fetal bovine serum (FBS) in phosphate-buffered saline (PBS) for 10 min, then transferred into 17.5% EG, 17.5% DMSO, 0.25 M trehalose and 10% FBS in PBS. They were placed on hemi-straws and inserted into 0.5 ml straws inside a previously cooled aluminum cylinder. Slow freezing was done in straws by the conventional method.

Results

Vitrified embryos had significantly higher survival, further cleavage and blastocyst formation rates than those in the slow freezing group (p < 0.001) and were comparable to controls. Blastocysts in the vitrification and control groups had significantly more cells than those in the slow freezing group (p < 0.05).

Conclusions

Closed-system vitrification was more effective than conventional slow freezing.

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Acknowledgements

This study was supported by a grant from the Faculty of Medicine Endowment Fund for Medical Research, Chiang Mai University, Thailand.

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Authors and Affiliations

  1. Division of Reproductive Medicine, Department of Obstetrics and Gynecology, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand

    Teraporn Vutyavanich, Opas Sreshthaputra, Waraporn Piromlertamorn & Siriporn Nunta

Authors
  1. Teraporn Vutyavanich
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  2. Opas Sreshthaputra
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  3. Waraporn Piromlertamorn
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  4. Siriporn Nunta
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Corresponding author

Correspondence to Teraporn Vutyavanich.

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Capsule

Closed system solid surface vitrification was more effective than conventional slow freezing in the cryopreservation of mouse 2-cell embryos.

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Vutyavanich, T., Sreshthaputra, O., Piromlertamorn, W. et al. Closed-system solid surface vitrification versus slow programmable freezing of mouse 2-cell embryos. J Assist Reprod Genet 26, 285–290 (2009). https://doi.org/10.1007/s10815-009-9324-8

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  • DOI: https://doi.org/10.1007/s10815-009-9324-8

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