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Successful twin birth following blastocyst culture of embryos derived from the immotile ejaculated spermatozoa from a patient with primary ciliary dyskinesia: A case report

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Abstract

Purpose

To describe the ultrastructure of spermatozoa from a patient with complete asthenozoospermia that resulted in live births following blastocyst culture.

Materials and methods

Analyses of spermatozoa from a 36 year old patient were performed using light and electron microscopy. The hypo-osmotic swelling test was used to select spermatozoa for intracytoplasmic sperm injection. Embryos were cultured to the blastocyst stage.

Results

100% of the spermatozoa had dynein arm deficiency with secondary defects varying from 3–17%. Six oocytes were injected; five fertilized normally and one was digynic. All five zygotes formed good quality blastocysts. Three blastocysts were cryopreserved and two blastocysts were transferred. Twin females were born at 37 weeks.

Conclusions

The hypo-osmotic swelling test can be used to select viable immotile ejaculated spermatozoa from a patient with dynein arm deficiency and can produce excellent fertilization rates and blastocyst development resulting in live births.

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Acknowledgements

Special thanks to Amanda Rabka for assistance with the semen analyses and the spermatozoa preparation.

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Authors and Affiliations

  1. Advanced Fertility and Reproductive Endocrinology Institute, LLC, 2728 Sunset Blvd, Suite 305, West Columbia, SC, 29169, USA

    Richard J. Kordus & Gail F. Whitman-Elia

  2. Cell and Developmental Biology and Anatomy Department, University of South Carolina School of Medicine, Bldg 1, Rm B-60, 6311 Garners Ferry Rd., Columbia, SC, 29208, USA

    Richard J. Kordus, Robert L. Price & Jeffrey M. Davis

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  1. Richard J. Kordus
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  2. Robert L. Price
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  3. Jeffrey M. Davis
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Correspondence to Richard J. Kordus.

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Capsule We report the ultrastructure of spermatozoa from a primary ciliary dyskinesia patient with dynein arm deficiency that resulted in a twin birth following blastocyst culture.

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Kordus, R.J., Price, R.L., Davis, J.M. et al. Successful twin birth following blastocyst culture of embryos derived from the immotile ejaculated spermatozoa from a patient with primary ciliary dyskinesia: A case report. J Assist Reprod Genet 25, 437–443 (2008). https://doi.org/10.1007/s10815-008-9254-x

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  • DOI: https://doi.org/10.1007/s10815-008-9254-x

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