Abstract
Purpose
Identification of the unique genes playing critical roles in human embryo cleavage.
Methods
Isolation of human ePAB cDNA using human ovary cDNA libraries and mouse ePAB amino acid sequences, followed by analysis of its expression pattern in various adult tissues and stages during early oocyte development excluding ePABP2.
Results
Human ePAB encodes a 330-aa protein and is located on chromosome 20q12-q13.1. The amino acid sequence is 72% homologous with that of mouse ePab. Human ePAB has only three RRMs and lacks a PABP domain; the expression pattern is nonspecific in adult tissues and detected in all stages, from oocyte to blastocyst. Human ePABP2 encodes a 282-aa protein and is located on chromosome 16q24.3. The amino acid sequence is 68% homologous with mouse ePabp2.
Conclusions
We identified human ePAB and ePABP2 cDNA. Human ePAB cDNA is not expressed specific to the ovary. Biological discrepancies exist between the human and the mouse.
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Acknowledgments
This study was supported by a Grant-in-Aid for Scientific Research (No. 19591887) from the Ministries of Education, Culture, Sports, Science and Technology and Health, Labour and Welfare of Japan.
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We successfully isolated the human ePAB and ePABP2 cDNAs and analyzed the expression patterns in humans.
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Sakugawa, N., Miyamoto, T., Sato, H. et al. Isolation of the human ePAB and ePABP2 cDNAs and analysis of the expression patterns. J Assist Reprod Genet 25, 215–221 (2008). https://doi.org/10.1007/s10815-008-9220-7
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DOI: https://doi.org/10.1007/s10815-008-9220-7