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Developmental prognosis for zygotes based on pronuclear pattern: Usefulness of pronuclear scoring

  • Assisted Reproduction
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Abstract

Purpose: To relate pronuclear patterns (PN) and zygote cytoplasmic appearance and embryo morphology.

Methods: The usefulness of PN classification described by Tesarik et al. 1999 (patterns p0-5) and Scott et al. 2000 (Z1-4), for embryo selection is assessed.

Results: Sinchrony on polarization and number of nucleolar precursor bodies (NPB) were associated with good quality embryos (p0 60.9% and p3 67.3%, and Z1 62.5% and Z2 64.7%; p<0.01). Pattern 4 zygotes were associated with small number of NPB developed into multinucleated embryos (14.3%) and poor quality embryos (61.9%). No significant differences were found in the pregnancy rate between transfer of at least one good prognosis PN pattern and transfer of poor prognosis PN patterns, although 75% of the transfers included at least one embryo derived from a pattern 0 zygote, and 55% included embryos from categories Z1 or Z2.

Conclusions: Sequential assessment involving the evaluation of oocyte quality, the classification of PN patterns and embryo morphology allows a more accurate evaluation of embryos to be selected for transfer.

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Acknowledgements

This work was carried out under the auspices of the Catedra de Investigación de Obstetricia y Ginecología del Institut Universitari Dexeus

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Authors and Affiliations

  1. Servei Medecina de la Reproducció, Dpt Obstetrícia i Ginecologia, Institut Universitari Dexeus, Passeig Bonanova, 89-91, 08017, Barcelona, Spain

    Gemma Arroyo, Anna Veiga & Pere Nolasc Barri

  2. Unitat de Biologia Cel.lular, Facultat de Ciències, Universitat Autónoma de Barcelona, 08193, Bellaterra, Barcelona, Spain

    Josep Santaló

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  1. Gemma Arroyo
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  2. Anna Veiga
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  4. Pere Nolasc Barri
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Correspondence to Gemma Arroyo.

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Arroyo, G., Veiga, A., Santaló, J. et al. Developmental prognosis for zygotes based on pronuclear pattern: Usefulness of pronuclear scoring. J Assist Reprod Genet 24, 173–181 (2007). https://doi.org/10.1007/s10815-006-9099-0

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  • DOI: https://doi.org/10.1007/s10815-006-9099-0

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