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Physiological responses of the red algae Gracilaria caudata (Gracilariales) and Laurencia catarinensis (Ceramiales) following treatment with a commercial extract of the brown alga Ascophyllum nodosum (AMPEP)

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Abstract

Extracts of brown algae are used commercially for agricultural crops due to their plant growth-promoting effects and they can also be applied to help improve seaweed cultivation. Gracilaria caudata is commercially exploited as a raw material for agar production in Brazil, and Laurencia catarinensis is a source of halogenated terpenoids with several biological activities. Investigations to understand the development of seaweeds of biotechnological interest are required in order to improve their cultivation and the sustainability for biomass production on an industrial scale. The aim of the present study was to evaluate the effects of Ascophyllum/Acadian marine plant extract powder (AMPEP), a commercial, water-soluble extract of the brown alga Ascophyllum nodosum, on the growth, branching development, and pigment content of G. caudata and L. catarinensis. Four concentrations of AMPEP (0, 0.1, 1.0, and 5.0 mg L−1) were added to sterilized seawater (seawater+AMPEP), and to a medium comprised of half-strength von Stosch solution (VSES/2+AMPEP). Experiments were performed under conditions of 23 ± 2 °C, a light:dark cycle of 14:10 h, salinity at 32 psu, and photon flux densities of 60 ± 10 μmol photons m−2 s−1. Data were analyzed by one-way ANOVA, followed by a posteriori multiple comparison, Student-Newman-Keuls’ test. Treatments of seawater+AMPEP at concentrations of 0.1 and 1.0 mg L−1 stimulated the growth rates of G. caudata when compared to the control. However, the effects of seawater+AMPEP and VSES/2+AMPEP on the branching architecture of the thalli were not significant for either species. Concentrations of phycoerythrin, phycocyanin, allophycocyanin, and chlorophyll-a in L. catarinensis were stimulated by the highest concentration of VSES/2+AMPEP (i.e., 5.0 mg L−1). This is the first study to report the stimulatory effect of AMPEP on the phycobiliprotein contents of selected red algae. Furthermore, seawater+AMPEP in concentrations of 0.1 and 1.0 mg L−1 stimulated growth rates of G. caudata, which could be applied in its large-scale cultivation. The results obtained for G. caudata and L. catarinensis, an agar-producing and a terpenoid-producing species, respectively, contributed to extending the knowledge of the beneficial uses of a brown seaweed-derived extract with biostimulatory properties for use with cultivated, economically important red algae.

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References

  • Ali MKM, Sani MZB, Hi KK, Yasir SM, Keon KH, Critchley AT, Hurtado AQ (2018a) The comparative efficiency of a brown algal-derived biostimulant extract (AMPEP), with and without supplemented PGRs: the induction of direct, axis shoots as applied to the propagation of vegetative seedlings for the successful mass cultivation of three commercial strains of Kappaphycus in Sabah, Malaysia. J Appl Phycol 30:1913–1919

    Article  CAS  Google Scholar 

  • Ali MKM, Yasir SM, Critchley AT, Hurtado AQ (2018b) Impacts of Ascophyllum marine plant extract powder (AMPEP) on the growth, incidence of the endophyte Neosiphonia apiculata and associated carrageenan quality of three commercial cultivars of Kappaphycus. J Appl Phycol 30:1185–1195

    Article  CAS  Google Scholar 

  • Armisen R (1995) World-wide use and importance of Gracilaria. J Appl Phycol 7:231–243

    Article  Google Scholar 

  • Borlongan IAG, Tibubos KR, Yunque DAT, Hurtado AQ, Critchley AT (2011) Impact of AMPEP on the growth and occurrence of epiphytic Neosiphonia infestation on two varieties of commercially cultivated Kappaphycus alvarezii grown at different depths in the Philippines. J Appl Phycol 23:615–621

    Article  Google Scholar 

  • Fujii MT, Cassano V, Stein EM, Carvalho LR (2011) Overview of the taxonomy and of the major secondary metabolites and their biological activities related to human health of the Laurencia complex (Ceramiales, Rhodophyta) from Brazil. Rev Bras Farmacogn 21:268–282

    Article  CAS  Google Scholar 

  • Guiry MD, Guiry GM (2018) Algaebase. World-wide electronic publication, National University of Ireland, Galway, http://www.algaebase.org; searched on 18 July 2018

  • Hurtado AQ, Critchley AT (2018) A review of multiple biostimulant and bioeffector benefits of AMPEP, an extract of the brown alga Ascophyllum nodosum, as applied to the enhanced cultivation and micropropagation of the commercially important red algal carrageenophyte Kappaphycus alvarezii and its selected cultivars. J Appl Phycol 30:2859–2873

    Article  CAS  Google Scholar 

  • Hurtado AQ, Yunque DA, Tibubos K, Critchley AT (2009) Use of Acadian marine plant extract powder from Ascophyllum nodosum in tissue culture of Kappaphycus varieties. J Appl Phycol 21:633–639

    Article  Google Scholar 

  • Jeffrey SW, Humphrey GF (1975) New spectrophotometric equations for determining chlorophylls a, b, c 1 and c 2 in higher plants, algae and natural phytoplankton. Biochem Physiol Pflanz 167:191–194

    Article  CAS  Google Scholar 

  • Khan W, Rayirath UP, Subramanian UP, Jitesh MN, Rayorath P, Hodges DM, Critchley AT, Craigie JS, Norrie J, Prithiviraj B (2009) Seaweed extracts as biostimulants of plant growth and development. J Plant Growth Regul 28:386–399

    Article  CAS  Google Scholar 

  • Kursar TA, Van Der Meer J, Alberte RS (1983) Light-harvesting system of red alga Gracilaria tikvahiae I. Biochemical analyses of pigment mutations. Plant Physiol 73:353–360

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lhullier C, Falkenberg M, Ioannou E, Quesada A, Papazafiri P, Horta PA, Schenkel EP, Vagias C, Roussis V (2010) Cytotoxic halogenated metabolites from the Brazilian red alga Laurencia catarinensis. J Nat Prod 73:27–32

    Article  CAS  PubMed  Google Scholar 

  • Loureiro RR, Reis RP, Berrogain FD, Critchley AT (2012) Extract powder from the brown alga Ascophyllum nodosum (Linnaeus) Le Jolis (AMPEP): a vaccine-like effect on Kappaphycus alvarezii (Doty) Doty ex P.C. Silva. J Appl Phycol 24:427–432

    Article  Google Scholar 

  • Loureiro RR, Reis RP, Berrogain FD, Critchley AT (2014) Effects of a commercial extract of the brown alga Ascophyllum nodosum on the biomass production of Kappaphycus alvarezii (Doty) Doty ex P.C. Silva and its carrageenan yield and gel quality cultivated in Brazil. J Appl Phycol 26:763–768

    Article  Google Scholar 

  • McDermid KJ (1988) Section V, Laurencia (Rhodophyta, Rhodomelaceae) introduction. In: Abbott IA (ed) Taxonomy of economic seaweeds, with reference to some Pacific and Caribbean species, vol 2. California Sea Grant College Program, University of California, La Jolla, pp 221–229

    Google Scholar 

  • Oliveira EC (1998) The seaweed resources of Brazil. In: Critchley AT, Ohno M (eds) Seaweed resources of the world. Japan International Cooperation Agency, Yokosuka, pp 366–371

    Google Scholar 

  • Oliveira EC, Paula EJ, Plastino EM, Petti R (1995) Metodologias para o cultivo no axênico de macroalgas marinas in vitro. In: Alveal K, Ferrario ME, Oliveira EC, Sar E (eds) Manual de Métodos Ficológicos. Universidad de Concepción, Concepción, pp 429–455

    Google Scholar 

  • Oliveira EC, Alveal K, Anderson R (2000) Mariculture of the agar-producing gracilarioid red algae. Rev Fish Sci 8:345–378

    Article  CAS  Google Scholar 

  • Plastino EM, Oliveira EC (1997) Gracilaria caudata J. Agardh (Gracilariales, Rhodophyta) - restoring an old name for a common western Atlantic algae. Phycologia 36:225–232

    Article  Google Scholar 

  • Reitz SR, Trumble JT (1996) Cytokinin-containing seaweed extract does not reduce damage by an insect herbivore. Hortscience 31:102–105

    Article  Google Scholar 

  • Tibubos KR, Hurtado AQ, Critchley AT (2017) Direct formation of axes in new plantlets of Kappaphycus alvarezii (Doty) Doty, as influenced by the use of AMPEP K+, spindle inhibitors, and plant growth hormones. J Appl Phycol 29:2345–2349

    Article  CAS  Google Scholar 

  • Wally OSD, Critchley AT, Hiltz D, Craigie JS, Han X, Zaharia LI, Abrams SR, Prithiviraj B (2013) Regulation of phytohormone biosynthesis and accumulation in Arabidopsis following treatment with commercial extract from the marine macroalga Ascophyllum nodosum. J Plant Growth Regul 32:324–339

    Article  CAS  Google Scholar 

  • Yokoya NS (2000) Apical callus formation and plant regeneration controlled by plant growth regulators on axenic cultures of the red alga Gracilariopsis tenuifrons (Gracilariales, Rhodophyta). Phycol Res 48:133–142

    Article  CAS  Google Scholar 

  • Yong YS, Yong WTL, Anton A (2013) Analysis of formulae for determination of seaweed growth rate. J Appl Phycol 25:1831–1834

    Article  Google Scholar 

  • Yunque DAT, Tibubos KR, Hurtado AQ, Critchley AT (2011) Optimization of culture conditions for tissue culture production of young plantlets of carrageenophyte Kappaphycus. J Appl Phycol 23:433–438

    Article  Google Scholar 

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Acknowledgements

The sample of AMPEP was a donation from Acadian Seaplants Limited, Canada. The authors would like to thank the journal reviewers for constructive feedback in the production of this manuscript.

Funding

The authors thank Conselho Nacional de Desenvolvimento Científico e Tecnológico for scholarship to JMCS and grant to NSY (CNPq Proc. 310672/2016-3), and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior CAPES/AUXPE-CIMAR 1991/2014, Proc. 23038.001431/2014-75) for grant and scholarship to JZC.

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Authors and Affiliations

  1. Instituto de Botânica, Secretaria de Estado do Meio Ambiente de São Paulo, Av. Miguel Estefano, 3687, São Paulo, SP, 04301-012, Brazil

    Jonatas M. C. Souza, Júlia Z. Castro & Nair S. Yokoya

  2. Verschuren Centre for Sustainability in Energy and the Environment, Cape Breton University, BIP 6L2, Sydney, Nova Scotia, Canada

    Alan T. Critchley

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  1. Jonatas M. C. Souza
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  2. Júlia Z. Castro
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  3. Alan T. Critchley
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  4. Nair S. Yokoya
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Correspondence to Nair S. Yokoya.

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Souza, J.M.C., Castro, J.Z., Critchley, A.T. et al. Physiological responses of the red algae Gracilaria caudata (Gracilariales) and Laurencia catarinensis (Ceramiales) following treatment with a commercial extract of the brown alga Ascophyllum nodosum (AMPEP). J Appl Phycol 31, 1883–1888 (2019). https://doi.org/10.1007/s10811-018-1683-z

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  • DOI: https://doi.org/10.1007/s10811-018-1683-z

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