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Selection and functional analysis of a Pyropia yezoensis ammonium transporter PyAMT1 in potassium deficiency

  • 22ND INTERNATIONAL SEAWEED SYMPOSIUM, COPENHAGEN
  • Published:
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Abstract

Seaweeds are believed to have developed unique mechanisms to maintain optimal cellular potassium and sodium concentrations in order to survive in the saline marine environment. To gain a molecular understanding of underlying potassium/sodium homeostasis in seaweeds, full-length cDNA libraries from the multiple stages in the life cycle, including gametophytes, conchosporangia and sporophytes of a marine red alga, Pyropia yezoensis, were constructed. A large portion of genes from each library through the life cycle was revealed to be functionally unknown reconfirming the uniqueness of P. yezoensis genes in terms of evolutionary lineage. Genes that could potentially contribute to potassium deficiency tolerance were selected from the potassium uptake defective Escherichia coli strain expressing gametophytes and conchosporangia libraries under the low potassium conditions. Of those, an ammonium transporter gene, PyAMT1, was demonstrated to enhance potassium deficiency tolerance effectively when expressed in the E. coli strain. Potential roles of PyAMT1 and other candidate components in this context are discussed.

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Acknowledgements

We would like to thank Ms. Takae Miyazaki and Mr. Hajime Takiguchi for taking care of the seaweed cultures, Dr. Yuko Makita and Dr. Minami Matsui (RIKEN Center for Sustainable Resource Science) for creating the P. yezoensis BLAST search engine and Dr. Megumu Takahashi (Tokyo University of Agriculture) for providing a photo of conchosporangium generation. We appreciate the contribution of the Marine Resources Research Center, Aichi Fisheries Research Institute in kindly providing the P. yezoensis strain U51 and Dr. Nobuyuki Uozumi (Tohoku University) for providing the E. coli TK2463 strain. Many thanks also go to Dr. Michael Adams for comments and discussion on the paper. This work was supported by funding from the RIKEN Incentive Research Projects.

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  1. RIKEN Center for Sustainable Resource Science, 1-7-22 Suehirocho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan

    Eri Adams & Ryoung Shin

  2. Faculty of Fisheries Sciences, Hokkaido University, 3-1-1 Minato-cho, Hakodate, Hokkaido, 041-8611, Japan

    Koji Mikami

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  1. Eri Adams
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Correspondence to Ryoung Shin.

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Adams, E., Mikami, K. & Shin, R. Selection and functional analysis of a Pyropia yezoensis ammonium transporter PyAMT1 in potassium deficiency. J Appl Phycol 29, 2617–2626 (2017). https://doi.org/10.1007/s10811-017-1196-1

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  • DOI: https://doi.org/10.1007/s10811-017-1196-1

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