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Effect of light quality on the growth and proximal composition of Amphora sp.

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Abstract

We determined the effects of various light spectra (white, blue, green, yellow, and Grolux) on the growth rate, proximate composition, pigment content, and cell size of the marine benthic diatom Amphora sp. during two growth phases of batch cultures. The growth rate was higher under green light and lowest with Grolux and yellow light. Cell size differed significantly between growth phases but remained unaffected by light spectra; the smallest cells were observed on the initial day of culture, whereas larger cells developed in the stationary phase under Grolux treatment. The proximate composition was modified by the light spectra and growth phase. In the exponential growth phase, the protein content was higher with yellow and white light, and lipid content peaked with Grolux. The pigment content (chlorophyll a, carotenoids) was also higher under yellow light. In the stationary growth phase, we noted a higher carbohydrate content under Grolux and yellow light. Our results show that light spectra and growth phase influence the metabolism of Amphora sp., changing its proximate composition, pigment content, growth rate, and cell size.

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Acknowledgements

This work has been funded by Consejo Nacional de Ciencia y Tecnología (CONACyT, project: SEP-CONACyT 2009-01-130074) and Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE, project: 623108). C.C. Romero-Romero acknowledges her Master’s in Science from CONACyT and CICESE.

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    Celia Carolina Romero-Romero & M. del Pilar Sánchez-Saavedra

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Romero-Romero, C.C., Sánchez-Saavedra, M. Effect of light quality on the growth and proximal composition of Amphora sp.. J Appl Phycol 29, 1203–1211 (2017). https://doi.org/10.1007/s10811-016-1029-7

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