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Identification and large isolation of an anti-inflammatory compound from an edible brown seaweed, Undariopsis peterseniana, and evaluation on its anti-inflammatory effect in in vitro and in vivo zebrafish

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Abstract

In this study, we isolated and purified secondary metabolites from an edible brown seaweed, Undariopsis peterseniana, and evaluated their anti-inflammatory effects in vitro and in vivo. Apo-9′-fucoxanthinone (Apo-9′), a main anti-inflammatory compound found in the chloroform fraction of U. peterseniana extracts, was first isolated by one-step centrifugal partition chromatography and identified by nuclear magnetic resonance. Apo-9′ significantly decreased nitric oxide (NO), prostaglandin E2, pro-inflammatory proteins (inducible NO synthase [iNOS] and cyclooxygenase [COX-2]), and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-6, and interleukin-1β) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells without exhibiting any cytotoxicity. In vivo, Apo-9′ protected LPS-stimulated zebrafish embryos against inflammatory stress and suppressed the expression of pro-inflammatory proteins such as COX-2 and iNOS. These findings suggest that U. peterseniana extract and Apo-9′ could be used as an anti-inflammatory agent in health care.

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Acknowledgements

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (No. 2014R1A1A2007015) and Ministry of Agriculture, Food and Rural Affairs (MAFRA), Ministry of Oceans and Fisheries (MOF), Rural Development Administration (RDA), and Korea Forest Service (KFS) (213004-04-4-SBA30).

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Authors and Affiliations

  1. Department of Marine Life science, School of Marine Biomedical Sciences, Jeju National University, Jeju, 690-756, Republic of Korea

    Ji-Hyeok Lee, Ju-Young Ko, Eun-A Kim & You-Jin Jeon

  2. Seaweed Research Institute, National Fisheries Research and Development Institute, Mokpo, 530-831, South Korea

    Eun-Kyoung Hwang

  3. Department of Marine Resources, Mokpo National University, Jeonnam, 534-729, South Korea

    Chan Sun Park

  4. Industry-Academic Cooperation Foundation, Jeju National University, Jeju, 690-756, South Korea

    Jung-Suck Lee

  5. Natural Product Research Center, Hanyang University, Daejeon-dong, Ansan, Gyeongi-do, Korea

    Chul-Young Kim

  6. Marine Natural Products Chemistry Laboratory, Korea Institute of Ocean Science and Technology, Ansan, 426-744, Republic of Korea

    Hyi-Seung Lee

  7. School of Medicine, Institute of Medical Sciences, Jeju National University, Jeju, 690-756, South Korea

    Hee-Kyoung Kang

  8. Department of Pharmacology, Ajou University School of Medicine, 164, Worldcup-ro, Yeongtong-gu, Suwon, 16499, South Korea

    Seon-Heui Cha

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  1. Ji-Hyeok Lee
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  2. Ju-Young Ko
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  5. Chan Sun Park
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  8. Hyi-Seung Lee
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Correspondence to Seon-Heui Cha or You-Jin Jeon.

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Lee, JH., Ko, JY., Kim, EA. et al. Identification and large isolation of an anti-inflammatory compound from an edible brown seaweed, Undariopsis peterseniana, and evaluation on its anti-inflammatory effect in in vitro and in vivo zebrafish. J Appl Phycol 29, 1587–1596 (2017). https://doi.org/10.1007/s10811-016-1012-3

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  • DOI: https://doi.org/10.1007/s10811-016-1012-3

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