Abstract
Saccharina latissima is an ecologically and economically important kelp species native to the coastal regions of the Northern Hemisphere. This species has considerable phylogeographic structure and morphological plasticity, but lack of resolution of available genetic markers prevents a finer characterization of its genetic diversity and structure. Here, we describe 12 microsatellite loci identified in silico in a genomic library, and assess their polymorphism in three distant populations. Allelic richness at the species level was relatively high (5–23 alleles per locus), as was gene diversity within populations (0.42 < H E < 0.62). In addition, individuals readily form distinct genotypic clusters matching their populations of origin. The variation detected confirms the great potential of these markers to investigate the biogeography and population dynamics of S. latissima, and to better characterize its genetic resources for the establishing farming industry.
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Acknowledgments
This work was funded by Portuguese Fundação para a Ciência e Tecnologia (FCT) through the project EXTANT (EXCL/AAG-GLO/0661/2012) and postdoctoral fellowship to JN (SFRH/BPD/88935/2012), and through the European Union’s Seventh Framework Programme, grant agreement no. 226248—Arctic Tipping Points (ATP).
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Paulino, C., Neiva, J., Coelho, N.C. et al. Characterization of 12 polymorphic microsatellite markers in the sugar kelp Saccharina latissima . J Appl Phycol 28, 3071–3074 (2016). https://doi.org/10.1007/s10811-016-0811-x
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DOI: https://doi.org/10.1007/s10811-016-0811-x