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Lipid digestibility and performance index of Litopenaeus vannamei fed with Chaetoceros muelleri cultured in two different enriched media

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Abstract

The in vitro digestibility of lipids from Chaetoceros muelleri that were cultured in Guillard medium (F/2) and agricultural fertilizer medium (AF/2) and performance index was examined in enzymatic extracts of Litopenaeus vannamei larvae. The highest enzymatic activity (EA) of lipases was observed in Zoea I (1.53 ± 0.14 EA/10 EU) and Mysis I (1.26 ± 0.01 EA/10 EU (EU are units of enzymatic activity)) when C. muelleri was grown in the F/2 medium. The highest concentration of the unsaturated fatty acids (UFAs) in C. muelleri was observed with F/2 medium (9.96 μg FA/106 cells), and the lowest was obtained with (AF/2) medium (6.68 μg FA/106 cells). The percentage of the total highly unsaturated fatty acids (HUFAs) in Zoea (18.23 %) and Mysis I (F/2 = 19.32 %, AF/2 = 20.25 %) larvae was similar between culture media. The performance indices (PI) of shrimp larvae (Zoea I to Mysis I) differed between culture media, with higher values of PI recorded with the F/2 medium-cultured algae (14.3 ± 1.1) with respect to the values with the AF/2 medium-cultured algae (8.0 ± 1.8). We concluded that due to the effect of the nitrogen source in AF/2 medium, C. muelleri cells have a lower HUFA content and L. vannamei larvae experience decreased lipid digestibility and performance index.

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Acknowledgments

This study was founded by the CICESE (623108) and SEP-CONACyT (130074) and to UABCS (AICM-CTM-04-11-25-11-05-24) projects. Thanks to Patricia Hinojosa for her technical support at the Comparative Physiology Laboratory of the Centro de Investigaciones Biológicas del Noroeste (CIBNOR). We would like to thank “AQUALAB” for supplying the shrimp larvae.

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Authors and Affiliations

  1. Universidad Autónoma de Baja California Sur (UABCS), Unidad Pichilingue, Apartado Postal 19-B, C.P. 23080, La Paz, Baja California Sur, Mexico

    Juan M. Pacheco-Vega & Marco A. Cadena-Roa

  2. Departamento de Acuicultura, Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Carretera Ensenada-Tijuana Número 3918, Zona Playitas, C.P. 22860, Ensenada, Baja California, Mexico

    M. del Pilar Sánchez-Saavedra

  3. Centro de Investigaciones Biológicas del Noroeste (CIBNOR, S.C.) Instituto Politécnico Nacional 195, Playa Palo de Santa Rita Sur, C.P. 23096, La Paz, B.C.S, Mexico

    Dariel Tovar-Ramírez

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  1. Juan M. Pacheco-Vega
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  2. M. del Pilar Sánchez-Saavedra
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  3. Marco A. Cadena-Roa
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Correspondence to Juan M. Pacheco-Vega.

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Pacheco-Vega, J.M., Sánchez-Saavedra, M.d.P., Cadena-Roa, M.A. et al. Lipid digestibility and performance index of Litopenaeus vannamei fed with Chaetoceros muelleri cultured in two different enriched media. J Appl Phycol 28, 2379–2385 (2016). https://doi.org/10.1007/s10811-015-0750-y

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  • DOI: https://doi.org/10.1007/s10811-015-0750-y

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