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Factors influencing the induction of adventitious bud and callus in the brown alga Sargassum horneri (Turner) C. Agardh

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Abstract

A high frequency of callus induction and propagation from leaf explants of the brown alga Sargassum horneri was achieved within 2 months of culture when grown in medium supplemented with 5 μM uniconazole, which is a triazole-type inhibitor of cytochrome P450 enzymes. Adventitious buds were efficiently formed from the pigmented callus after transfer to medium without uniconazole, indicating that treatment with uniconazole was more beneficial for regeneration of the alga. Favorable culture conditions for induction of adventitious buds and calli included temperatures of 15 to 25 °C and light levels of 20 to 200 μmol photons m−2 s−1. Blue light promoted the production of adventitious buds and calli. The frequency of formation of adventitious buds and calli in explants from thalli with one leaf was more than 90 %, while it was 10 % only when explants were sourced from thalli with 9 to 11 leaves. These findings will be useful for clonal propagation and storage of seed materials for mariculture of Sargassum species.

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Acknowledgments

We are grateful to Takashi Tani (Tetsugumi industry Co., Ltd, Japan) for kindly helping to collect mature thalli of S. horneri and to Dr. Hajime Yasui (Hokkaido University, Japan) for kindly providing microscopes and helpful discussion. This work was supported by a National Project for the Formation of Tohoku Marine Science Center (Innovation Strategy for the Industrial Seaweed Utilization Supporting Regional Seeds and Local Features of Sanriku Coast) grant from MEXT (Ministry of Education, Culture, Sports, Science, and Technology of Japan).

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Authors and Affiliations

  1. Faculty of Fisheries Sciences, Hokkaido University, Hakodate, 041-8611, Japan

    Toshiki Uji, Naotsune Saga & Kazuo Miyashita

  2. Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, 041-8611, Japan

    Daisuke Nanaumi

  3. Algatech Kyowa, Kyowa Concrete Industry Co. Ltd., Hakodate, Hokkaido, 040-0051, Japan

    Chikara Kawagoe

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  1. Toshiki Uji
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  2. Daisuke Nanaumi
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  4. Naotsune Saga
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  5. Kazuo Miyashita
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Correspondence to Kazuo Miyashita.

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Uji, T., Nanaumi, D., Kawagoe, C. et al. Factors influencing the induction of adventitious bud and callus in the brown alga Sargassum horneri (Turner) C. Agardh. J Appl Phycol 28, 2435–2443 (2016). https://doi.org/10.1007/s10811-015-0745-8

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  • DOI: https://doi.org/10.1007/s10811-015-0745-8

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