Abstract
Defatted microalgal biomass (DAB) of a local Scenedesmus dimorphus strain was characterised as animal feed in an experimental Wistar rat model. The DAB contained 21.5 % protein, 28 % dietary fibre and 21 % ash on dry basis. The DAB was rich in calcium (1208 mg 100 g−1 biomass) followed by magnesium (400.6 mg 100 g−1) among the tested mineral elements. The essential amino acids (EAA) constituted 48 % (w/w) of proteins, and the EAA index was similar to casein and soybean. The protein was limiting in sulphur-containing amino acids cysteine and methionine. The algal biomass showed 5–6-fold higher water absorption capacity (3.44 ± 0.06 g g−1 biomass) compared with basal diet (0.67 ± 0.06 g g−1 biomass). The DAB was found to be safe in both short-term (14 days), single-dose feeding (20 % (w/w) feed) and long-term (90 days) repeated-dose feeding (at 5 and 10 % (w/w) feed). In the short-term feeding trial, faecal output and water intake increased by 2-fold during the initial 4 days, mainly attributed to higher ash and fibre content of biomass. The long-term repeated-dose feeding of DAB did not induce any physiological changes in rats such as feed intake, haematology profile, serum biochemical parameters, histopathology and relative organ weight. The feed conversion ratio and quotient of protein efficiency ratio in DAB-fed groups were similar to control. However, animals required a longer adaptation period to DAB-mixed feed, as indicated by slower body weight gain and feed intake. The study suggests that DAB can be used as animal feed up to 10 % (w/w) replacement levels.
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References
Andersen RA, Berges JA, Harrisson PJ, Watanabe MM (2005) Recipies for freshwater and seawater media. In: Anderson RA (ed) Algal culturing techniques. Elsevier Academic Press, Amsterdam, pp 429–538
Association of Official Analytical Chemists (AOAC) (1997) 16th edn, Washington DC, USA. 1115 pp
Association of Official Analytical Chemists (AOAC) (2006) Method 975.03 B (b) Metals in plants and pet foods atomic absorption spectrophotometric method
Becker W (2004) Microalgae in human and animal nutrition. In: Richmond A (ed) Handbook of microalgal culture: biotechnology and applied phycology. Blackwell Science, Oxford, pp 312–351
Becker EW (2007) Micro-algae as a source of protein. Biotechnol Adv 25:207–210
Belay A, Kato T, Ota Y (1996) Spirulina (Arthrospira): potential application as an animal feed supplement. J Appl Phycol 8:303–311
Bharucha C, Meyer H, Mody A, Carman RH (1976) Handbook of medical laboratory technique. Wesley Press, Mysore, pp 53–59
Bidlingmeyer BA, Cohen SA, Tarvin TL (1984) Rapid analysis of amino acids using pre-column derivatization. J Chromatogr B 336:93–104
Dib MD, Engle TE, Han H, Roman-Muniz IN, Archibeque SL (2012) Effects of algal meal supplementation to finishing wethers on performance and carcass characteristics (abstract). J Anim Sci 90(3):148
DuBois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356
Dupuy HP, Fore SP (1970) Determination of residual solvent in oilseed meals and flours: II. Volatilization procedure. JAOCS 47:231–233
Fevrier C, Seve B (1975) Essais d’incorporation de spiruline (Spirulina maxima) dans les aliments des porcins. Ann Nutr Aliment 29:625–650
Herrero C, Abalde J, Fabregas J (1993) Nutritional properties of four marine microalgae for albino rats. J Appl Phycol 5:573–580
Hintz HF, Heitman H (1967) Sewage-grown algae as a protein supplement for swine. Anim Prod 9:135–140
Imafidon GI, Sosulski FW (1990) Nucleic acid nitrogen of animal and plant foods. J Agric Food Chem 38:118–120
Lahaye M (1991) Marine algae as source of fibres: determination of soluble and insoluble dietary contents in some “sea vegetables. J Sci Food Agr 54:587–594
Leng X, Hsu KN, Austic RE, Lei XG (2014) Effect of dietary defatted diatom biomass on egg production and quality of laying hens. J Anim Sci Biotechnol 5:3
Lipstein B, Hurwitz S (1980) The nutritional and economic value of algae for poultry. In: Shelef G, Soeder CJ (eds) Algae biomass production and use. Elsevier-North Holland, Amsterdam, pp 667–686
Lum KK, Kim J, Lei XG (2013) Dual potential of microalgae as a sustainable biofuel feedstock and animal feed. J Anim Sci Biotechnol 4:53
Mokady S, Yannai S, Einav P, Berk Z (1980) Protein nutritive value of several microalgae species for young chickens and rats. In: Shelef G, Soeder CJ (eds) Algae biomass production and use. Elsevier-North Holland, Amsterdam, pp 655–660
Moody JW, McGinty CM, Quinn JC (2014) Global evaluation of biofuel potential from microalgae. Proc Natl Acad Sci U S A 111:8691–8696
Moreira LM, Behling BS, Rodrigues RS, Costa JAV, Soares AS (2013) Spirulina as a protein source in the nutritional recovery of Wistar rats. Braz Arch Biol Technol 56:447–456
Mukherjee D, Bajaj H, Garg N, Abraham J (2013) Feeding a billion: role of the food processing industry. http://www.ficci.com/spdocument/20312/Feeding-a-Billion_Role-of-the-Food-Processing-Industry.pdf
Prosky L, Asp NG, Schweizer TF, Devries JW, Furda I (1988) Determination of insoluble, soluble, and total dietary fibre in foods and food products: interlaboratory study. J Assoc Off Anal Chem 71:1017–1023
Quinn JR, Paton D (1979) A practical measurement of water hydration capacity of protein materials. Cereal Chem 56:38–40
Ravishankar GA, Sarada R, Vidyashankar S, VenuGopal KS, Kumudha A (2012) Cultivation of micro-algae for lipids and hydrocarbons, and utilization of spent biomass for livestock feed and for bio-active constituents. In: Makkar HPS (ed) Biofuel coproducts as livestock feed—opportunities and challenges. FAO, Rome, pp 423–446
Reeves PG, Nielsen FH, Fahey GC Jr (1993) AIN-93 purified diets for laboratory rodents: final report of the American institute of nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 123:1939–1951
Rodolfi L, Zittelli CG, Bassi N, Padovani G, Biondi N, Bonini G, Tredici MR (2009) Micro-algae for oil: strain selection, induction of lipid synthesis and outdoor mass cultivation in a low cost photobioreactor. Biotechnol Bioeng 102:100–112
Ross E, Dominy W (1990) The nutritional value of dehydrated blue-green algae (Spirulina plantensis) for poultry. Poult Sci 69:794–800
Saeid A, Chojnacka K, Korczyński M, Korniewicz D, Dobrzański Z (2013) Biomass of Spirulina maxima enriched by biosorption process as a new feed supplement for swine. J Appl Phycol 25:667–675
Sander K, Murthy GS (2010) Life cycle analysis of algae biodiesel. Int J Life Cycle Assess 15:704–714
Sarat Chandra T, Suvidha G, Mukherji S, Chauhan VS, Vidyashankar S, Krishnamurthi K, Sarada R, Mudliar SN (2014) Statistical optimization of thermal pretreatment conditions for enhanced biomethane production from defatted algal biomass. Bioresour Technol 162:157–165
Schilter B, Andersson C, Anton R, Constable A, Kleiner J, O’Brien J, Renwick AG, Korver O, Smit F, Walker R (2003) Guidance for the safety assessment of botanicals and botanical preparations for use in food and food supplements. Food Chem Toxicol 41:1625–1649
Shields RJ, Lupatsch I (2012) Algae for aquaculture and animal feeds. J Anim Sci 21:23–27
Slade R, Bauen A (2013) Micro-algae cultivation for biofuels: cost, energy balance, environmental impacts and future prospects. Biomass Bioenerg 53:29–38
Sosulski FW, Humbert ES, Bui K, Jones JD (1976) Functional properties of rapeseed flours, concentrates and isolates. J Food Sci 41:1349–1352
Venkataraman LV, Becker EW (1985) Biotechnology and utilization of algae—the Indian experience. Department of Science and Technology, New Delhi, pp 186–207
Vidyashankar S, Deviprasad K, Chauhan VS, Ravishankar GA, Sarada R (2013) Selection and evaluation of CO2 tolerant indigenous microalga Scenedesmus dimorphus for unsaturated fatty acid rich lipid production under different culture conditions. Bioresour Technol 144:28–37
Walz OP, Brune H (1980) Studies on some nutritive effects of the green algae Scenedesmus acutus with pigs and broilers. In: Shelef G, Soeder CJ (eds) Algae biomass production and use. Elsevier-North Holland, Amsterdam, pp 733–744
Wijffels RA, Barbosa M (2010) An outlook of microalgal biofuels. Science 329:796–799
Yap TN, Wu JF, Pond WG, Krook L (1982) Feasibility of feeding Spirulina maxima, or Chlorella sp. to pigs weaned to a dry diet at 4 to 8 days of age. Nutr Reports Int 25:543–552
Yazdani SS, Gonzalez R (2007) Anaerobic fermentation of glycerol: a path to economic viability for the biofuels industry. Curr Opin Biotechnol 18:213–219
Acknowledgments
The authors thank Dr. R. Lalitha Gowda for the help in amino acid analysis. The financial assistance provided by KDMIPE-ONGC is gratefully acknowledged. VS acknowledges UGC, Govt. of India, for the award of senior research fellowship. The authors thank Director, CFTRI for encouragement.
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Vidyashankar, S., VenuGopal, K.S., Chauhan, V.S. et al. Characterisation of defatted Scenedesmus dimorphus algal biomass as animal feed. J Appl Phycol 27, 1871–1879 (2015). https://doi.org/10.1007/s10811-014-0498-9
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DOI: https://doi.org/10.1007/s10811-014-0498-9