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Antioxidant activities and polyphenolics of various solvent extracts of red seaweed, Gracilaria changii

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Abstract

This study determined the levels of polyphenols, flavonoids, carotenoids and antioxidant activity of the edible red seaweed, Gracilaria changii. Freeze-dried G. changii powder was extracted using five solvent systems: 80 % ethanol, 80 % methanol, 80 % acetone, ethyl acetate and water. The antioxidant activity of the extracts was measured using three assays, namely 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing power (FRAP) and β-carotene bleaching assays (BCB). The total phenolics (TPC) and total flavonoids content (TFC) and the total carotenoids content (TCC) were also determined. Ethanol extract had the highest yield followed by methanol > water > acetone > ethyl acetate extract. The radical scavenging effects of all the extracts showing dose-dependency properties with the ethyl acetate extract of G. changii exhibiting the highest radical scavenging effect (EC50 of 0.51 ± 0.09 mg mL−1), while the water extract had the lowest scavenging effect of 7.80 ± 0.57 mg mL−1. Ferric reducing power was in the following descending order: ethyl acetate > ethanol > methanol > acetone > water. TPC was highest in the ethyl acetate extract (21.57 ± 2.58 mg PGE g−1) and least in the water extract (6.06 ± 0.52 mg PGE g−1). The TFC of G. changii was in the range of 18.97 ± 0.89–200.87 ± 3.61 mg RE g−1. The results revealed the effect of different extracting solvents in altering the antioxidant potential of G.changii, and ethyl acetate was identified as the most efficient solvent for extracting bioactive compounds from this red seaweed.

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References

  • Abbott IA, Junfu Z, Bangmei X (1991) Gracilaria mixta, sp. nov. and other Western Pacific species of the Genus (Rhodophyta : Gracilariaceae). Pac Sci 45:12–27

    Google Scholar 

  • Airanthi MKW, Hosokawa M, Miyashita K (2011) Comparative antioxidant activity of edible Japanese brown seaweeds. J Food Sci 76:C106–C111

    Article  Google Scholar 

  • Akter MS, Ahmed M, Eun J-B (2010) Solvent effects on antioxidant properties of persimmon (Diospyros kaki L. cv. Daebong) seeds. Int J Food Sci Technol 45:2258–2264

    Article  CAS  Google Scholar 

  • Armisen R (1995) World-wide use and importance of Gracilaria. J Appl Phycol 7:231–243

    Article  Google Scholar 

  • Auezova L, Najjar F, Selivanova O (2013) Antioxidant activity of brown alga Saccharina bongardiana from Kamchatka (Pacific coast of Russia). A methodological approach. J Appl Phycol 25:1189–1196

  • Benzie IF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem 239:70–76

    Article  CAS  PubMed  Google Scholar 

  • Boeing JS, Barizão EO, E Silva BC, Montanher PF, Almeida VDC, Visentainer JV (2014) Evaluation of solvent effect on the extraction of phenolic compounds and antioxidant capacities from the berries: application of principal component analysis. Chem Cent J 8:48–56

    Article  PubMed Central  PubMed  Google Scholar 

  • Brand-Williams W, Cuvelier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. LWT Food Sci Technol 28:25–30

    Article  CAS  Google Scholar 

  • Brighente IMC, Dias M, Verdi LG, Pizzolatti MG (2007) Antioxidant cctivity and total phenolic content of some Brazilian species. Pharm Biol 45:156–161

    Article  CAS  Google Scholar 

  • Çelik SE, Ozyürek M, Güçlü K, Apak R (2010) Solvent effects on the antioxidant capacity of lipophilic and hydrophilic antioxidants measured by CUPRAC, ABTS/persulphate and FRAP methods. Talanta 81:1300–1309

    Article  PubMed  Google Scholar 

  • Chan PT, Matanjun P, Yasir SM, Tan TS (2014) Antioxidant and hypolipidaemic properties of red seaweed, Gracilaria changii. J Appl Phycol 26:987–997

    Article  CAS  Google Scholar 

  • Chang C-C, Yang M-H, Wen H-M, Chern J-C (2002) Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 10:178–182

    CAS  Google Scholar 

  • Chew YL, Lim YY, Omar M, Khoo KS (2008) Antioxidant activity of three edible seaweeds from two areas in South East Asia. LWT Food Sci Technol 41:1067–1072

    Article  CAS  Google Scholar 

  • Cho M, Lee H-S, Kang I-J, Won M-H, You SG (2011) Antioxidant properties of extract and fractions from Enteromorpha prolifera, a type of green seaweed. Food Chem 127:999–1006

    Article  CAS  PubMed  Google Scholar 

  • Christaki E, Bonos E, Giannenas I, Florou-Paneri P (2012) Functional properties of carotenoids originating from algae. J Sci Food Agric 93:5–11

    Article  PubMed  Google Scholar 

  • Connan S, Goulard F, Stiger V, Deslandes E, Gall EA (2004) Interspecific and temporal variation in phlorotannin levels in an assemblage of brown algae. Bot Mar 47:410–416

    Article  CAS  Google Scholar 

  • Ganesan P, Kumar CS, Bhaskar N (2008) Antioxidant properties of methanol extract and its solvent fractions obtained from selected Indian red seaweeds. Bioresour Technol 99:2717–2723

    Article  CAS  PubMed  Google Scholar 

  • Holdt SL, Kraan S (2011) Bioactive compounds in seaweed : functional food applications and legislation. J Appl Phycol 23:543–597

    Article  CAS  Google Scholar 

  • Hsu C-L, Wu C-H, Huang S-L, Yen G-C (2009) Phenolic compounds rutin and o-coumaric acid ameliorate obesity induced by high-fat diet in rats. J Agric Food Chem 57:425–431

    Article  CAS  PubMed  Google Scholar 

  • Ismail A, Tan S (2002) Antioxidant activity of selected commercial seaweeds. Malays J Nutr 8:167–177

    PubMed  Google Scholar 

  • Je J-Y, Park P-J, Kim E-K, Park J-S, Yoon H-D, Kim K-R, Ahn C-B (2009) Antioxidant activity of enzymatic extracts from the brown seaweed Undaria pinnatifida by electron spin resonance spectroscopy. LWT Food Sci Technol 42:874–878

    Article  CAS  Google Scholar 

  • Jiménez-Escrig A, Sánchez-Muniz F (2000) Dietary fibre from edible seaweeds: chemical structure, physiochemical properties and effects on cholesterol metabolism. Nutr Res 20:585–598

    Article  Google Scholar 

  • Jiménez-Escrig A, Gómez-Ordóñez E, Rupérez P (2011) Brown and red seaweeds as potential sources of antioxidant nutraceuticals. J Appl Phycol 24:1123–1132

    Article  Google Scholar 

  • Khoo HE, Ismail A, Mohd-Esa N, Idris S (2008) Carotenoid content of underutilized tropical fruits. Plant Foods Hum Nutr 63:170–175

    Article  CAS  PubMed  Google Scholar 

  • Kong WK, Mat-Junit S, Aminudin N, Ismail A, Aziz AA (2012) Antioxidant activities and polyphenolics from the shoots of Barringtonia racemosa (L.) Spreng in a polar to apolar medium system. Food Chem 134:324–332

    Article  CAS  Google Scholar 

  • Kumar KS, Ganesan K, Rao PVS (2008) Antioxidant potential of solvent extracts of Kappaphycus alvarezii (Doty) Doty – an edible seaweed. Food Chem 107:289–295

    Article  CAS  Google Scholar 

  • Lin W-T, Huang C-C, Lin T-J, Chen J-R, Shieh M-J, Peng H-C, Yang S-C, Huang C-Y (2009) Effects of beta-carotene on antioxidant stauts in rats with chronic alcohol consumption. Cell Biochem Funct 27:344–350

    Article  CAS  PubMed  Google Scholar 

  • López A, Rico M, Rivero A, Suárez de Tangil M (2011) The effects of solvents on the phenolic contents and antioxidant activity of Stypocaulon scoparium algae extracts. Food Chem 125:1104–1109

    Article  Google Scholar 

  • Maisuthisakul P, Pongsawatmanit R (2004) Effect of sample preparation methods and extraction time on yield and antioxidant activity from Kradonbok (Careya sphaerica Roxb.) leaves. Kasetsart J (Nat Sci) 14:8–14

    Google Scholar 

  • Matanjun P, Mohamed S, Mustapha NM, Muhammad K, Cheng HM (2008) Antioxidant activities and phenolics content of eight species of seaweeds from north Borneo. J Appl Phycol 20:367–373

    Article  CAS  Google Scholar 

  • Matsukawa R, Dubinsky Z, Kishimoto E, Masaki K, Masuda Y, Takeuchi T, Chihara M, Yamamoto Y, Niki E, Karube I (1997) A comparison of screening methods for antioxidant activity in seaweeds. J Appl Phycol 9:29–35

    Article  CAS  Google Scholar 

  • Mendonça-Filho RR (2006) Bioactive Phytocompounds: New Approaches in the Phytosciences. In: Ahmad I, Aqil F, Owais M (eds) Modern phytomedicine. Turning medicinal plants into drugs. Wiley-VCH, Weinheim, pp 1–24

    Google Scholar 

  • Namvar F, Mohamed S, Fard SG et al (2012) Polyphenol-rich seaweed (Eucheuma cottonii) extract suppresses breast tumour via hormone modulation and apoptosis induction. Food Chem 130:376–382

    Article  CAS  Google Scholar 

  • O’Sullivan AM, O’Callaghan YC, O’Grady MN, Queguineur B, Hanniffy D, Troy DJ, Kerry JP, O’Brien NM (2011) In vitro and cellular antioxidant activities of seaweed extracts prepared from five brown seaweeds harvested in spring from the west coast of Ireland. Food Chem 126:1064–1070

    Article  Google Scholar 

  • Othman A, Ismail A, Abdul Ghani N, Adenan I (2007) Antioxidant capacity and phenolic content of cocoa beans. Food Chem 100:1523–1530

    Article  CAS  Google Scholar 

  • Pérez-Jiménez J, Saura-Calixto F (2006) Effect of solvent and certain food constituents on different antioxidant capacity assays. Food Res Int 39:791–800

    Article  Google Scholar 

  • Phang S-M, Shaharuddin S, Noraishah H, Sasekumar A (1996) Studies on Gracilaria changii (Gracilariales, Rhodophyta) from Malaysian mangroves. Hydrobiologia 326–327:347–352

    Article  Google Scholar 

  • Piccolella S, Fiorentino A, Pacifico S, D’abrosca B, Uzzo P, Monaco P (2008) Antioxidant properties of sour cherries (Prunus cerasus L.): role of colorless phytochemicals from the methanolic extract of ripe fruits. J Agric Food Chem 56:1928–1935

    Article  CAS  PubMed  Google Scholar 

  • Russo M, Tedesco I, Iacomino G, Palumbo R, Galano G, Russo GL (2005) Dietary phytochemicals in chemoprevention of cancer. Curr Med Chem Immunol Endocrinol Metab Agents 5:61–72

    Article  CAS  Google Scholar 

  • Sabeena Farvin KH, Jacobsen C (2013) Phenolic compounds and antioxidant activities of selected species of seaweeds from Danish coast. Food Chem 138:1670–1681

    Article  CAS  PubMed  Google Scholar 

  • Souza BWS, Cerqueira MA, Martins JT, Quintas MAC, Ferreira ACS, Teixeira JA, Vicente AA (2011) Antioxidant potential of two red seaweeds from the Brazilian coasts. J Agric Food Chem 59:5589–5594

    Article  CAS  PubMed  Google Scholar 

  • Stankovic MS, Niciforovic N, Topuzovic M, Solujic S (2011) Total phenolic content, flavonoid concentrations and antioxidant activity, of the whole plant and plant parts extracts from Teucrium Montanum L. Var. Montanum, F. Supinum (L.) Reichenb. Biotechnol Biotechnol Equip 25:2222–2227

    Article  Google Scholar 

  • Thoo YY, Ho SK, Liang JY et al (2010) Effects of binary solvent extraction system, extraction time and extraction temperature on phenolic antioxidants and antioxidant capacity from mengkudu (Morinda citrifolia). Food Chem 120:290–295

    Article  CAS  Google Scholar 

  • Velioglu YS, Mazza G, Gao L, Oomah BD (1998) Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agric Food Chem 46:4113–4117

    Article  CAS  Google Scholar 

  • Vijayabaskar P, Shiyamala V (2012) Antioxidant properties of seaweed polyphenol from Turbinaria ornata (Tuener) J. Agardh, 1848. Asian Pac J Trop Biomed 1–9

  • Wong JY, Chye FY (2009) Antioxidant properties of selected tropical wild edible mushrooms. J Food Compos Anal 22:269–277

    Article  CAS  Google Scholar 

  • Wong P-F, Tan L-J, Nawi H, AbuBakar S (2006) Proteomics of the Red Alga, Gracilaria Changii (Gracilariales, Rhodophyta). J Phycol 42:113–120

    Article  CAS  Google Scholar 

  • Yumiko YS, Hsieh YP, Takashi S (2003) Distribution of flavonoids and related compounds from seaweeds in Japan. J Tokyo Univ Fish 89:1–6

    Google Scholar 

  • Zakaria NA, Ibrahim D, Sulaiman SF, Supardy NA (2011) Assessment of antioxidant activity, total phenolic content and in vitro toxicity of Malaysian red seaweed, Acanthophora spicifera. J Chem Pharm Res 3:182–191

    CAS  Google Scholar 

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Acknowledgments

The authors thank the Fisheries Research Institute Malaysia Sarawak and the Seaweed Research Unit at Universiti Malaysia Sabah for supplying the seaweed. This study was funded by the Ministry of Science, Technology and Innovation of Malaysia (MOSTI), Project code: 06-01-10-SF0163.

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Authors and Affiliations

  1. Faculty of Food Science and Nutrition, Universiti Malaysia Sabah, Jalan UMS, 88400, Kota Kinabalu, Sabah, Malaysia

    Pei Teng Chan & Patricia Matanjun

  2. Faculty of Science and Natural Resources, Universiti Malaysia Sabah, Jalan UMS, 88400, Kota Kinabalu, Sabah, Malaysia

    Suhaimi Md Yasir

  3. Faculty of Medicine and Health Science, Universiti Malaysia Sabah, Jalan UMS, 88400, Kota Kinabalu, Sabah, Malaysia

    Tek Song Tan

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  1. Pei Teng Chan
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Correspondence to Patricia Matanjun.

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Chan, P.T., Matanjun, P., Yasir, S.M. et al. Antioxidant activities and polyphenolics of various solvent extracts of red seaweed, Gracilaria changii . J Appl Phycol 27, 2377–2386 (2015). https://doi.org/10.1007/s10811-014-0493-1

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