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UV-irradiation mutation of tetraspores of Gracilariopsis lemaneiformis and screening of thermotolerant strains

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Abstract

The rhodophyte Gracilariopsis lemaneiformis is one of the most economically important marine algae in China. In order to extend the cultivation period and enlarge the cultivation area, mutagenic breeding was used in this study for screening strains with thermal tolerance. The tetraspores were mutated by UV irradiation, and then thermotolerant strains were screened by exposing developing tetraspores to a range of higher temperatures. Two heat-tolerant mutants, MT-17 and MT-18, were obtained and physiologically compared with cultivar 981 and the wild strains. The results demonstrated that the two mutants grew at significantly higher rates than wild strains and similar to that of cultivar 981 both in the laboratory and the sea. The malondialdehyde contents in MT-17 and MT-18 were all significantly lower than the wild-type after 3-day heat stress, and that in MT-18 was lower than cultivar 981. The superoxide dismutase activities of MT-17 and MT-18 were significantly higher than the wild-type, and those of MT-18 and cultivar 981 were at the same level all through the treatment. The heat shock protein 70 gene expressions of two mutants was higher than the wild-type and that of MT-18 remained at the same level as that of cultivar 981 after 4 h heat shock. All these indicate that the two mutants were more tolerant to high temperature than the wild-type. RSAP (restriction site amplified polymorphism) analysis indicated the genetic background of the two mutants may be changed. The mutagenesis and selection process may help to develop heat-tolerant G. lemaneiformis cultivars in the future.

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Acknowledgments

This work was supported by the public welfare project of ‘The Twelfth Five-Year-Plan’ in National Science and Technology for the Rural Development in China (2012AA100811-3) and the Ministry of Agriculture of China (200903030).

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Authors and Affiliations

  1. Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China

    Feng Fu, Zheng-Hong Sui, Wei Zhou, Jin-Guo Wang, Lian-Peng Chang & Shu-Fang Ci

  2. Ocean College, Yantai University, Yantai, 264005, China

    Feng Fu

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  1. Feng Fu
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  2. Zheng-Hong Sui
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  3. Wei Zhou
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  4. Jin-Guo Wang
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Correspondence to Zheng-Hong Sui.

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Fu, F., Sui, ZH., Zhou, W. et al. UV-irradiation mutation of tetraspores of Gracilariopsis lemaneiformis and screening of thermotolerant strains. J Appl Phycol 26, 647–656 (2014). https://doi.org/10.1007/s10811-013-0087-3

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  • DOI: https://doi.org/10.1007/s10811-013-0087-3

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