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Biostimulant activity of brown seaweed species from Strangford Lough: compositional analyses of polysaccharides and bioassay of extracts using mung bean (Vigno mungo L.) and pak choi (Brassica rapa chinensis L.)

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Abstract

The aims of this study were to characterise the composition of seaweed species and to evaluate the efficacy of aqueous extracts as plant biostimulants. Five species (Ascophyllum nodosum, Fucus serratus, Fucus vesiculosus, Laminaria hyperborea and Sargassum muticum) of seaweed were harvested from Strangford Lough, Northern Ireland for the evaluation of polysaccharides, indole-3-acetic acid (IAA), carbon, nitrogen, lipid, ash and mineral contents. The compositional analyses of the five species and their freeze-dried extracts were also carried out using thermogravimetric analysis, scanning electron microscopy and X-ray microanalysis. The concentration of IAA in the acid extracts of the five species ranged between 2.74 and 46.8 ng g−1. The carbon, nitrogen, lipid and ash contents ranged between 25.0 and 38.6, 1.37%, and 3.16, 0.83%, and 3.98 and 18.10 and 47.68%, respectively. L. hyperborea and S. muticum contained the highest amounts of minerals. The biostimulant activities of acidic (pH 3.0), neutral (pH 6.5) and alkaline (pH 9.0) extracts were determined by mung bean bioassay. The alkaline extracts from F. vesiculosus and A. nodosum stimulated significantly (P < 0.001) higher dry matter (DM, %) yield of the mung bean plants. The majority of the acidic extracts significantly (P < 0.001) enhanced root formation on the mung bean stem cuttings compared to alkaline or neutral extracts. The acidic extracts of the five species, water control and a commercial product were evaluated as foliar feeds for pak choi plants using a hydroponic production system. The interaction of species, e.g. A. nodosum and F. vesiculosus and the two treatment dilutions on DM yield increases of pak choi were significant (P < 0.05).

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Acknowledgements

RS and FA would like to thank Dr. S Watson for the statistical design of experiments and data analysis. The authors also would like to thank Drs R Copeland and P Mercer and Mrs G Nicholl for their contributions.

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The authors have declared no conflict of interest.

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Authors and Affiliations

  1. Plant Health and Environment Protection Branch, Agri-Food and Biosciences Institute, Newforge lane, Belfast, BT9 5PX, UK

    S. H. S. Sharma, G. Lyons, D. McCall, E. Carmichael, F. Andrews & R. Swan

  2. Food Chemistry Branch, Agri-Food and Biosciences Institute, Newforge lane, Belfast, BT9 5PX, UK

    C. McRoberts & R. McCormack

  3. Northern Ireland Environment Agency, Belfast, BT30 6JB, UK

    R. Mellon

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  1. S. H. S. Sharma
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  2. G. Lyons
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  3. C. McRoberts
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  4. D. McCall
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  5. E. Carmichael
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  6. F. Andrews
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  7. R. Swan
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  8. R. McCormack
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  9. R. Mellon
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Correspondence to S. H. S. Sharma.

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Sharma, S.H.S., Lyons, G., McRoberts, C. et al. Biostimulant activity of brown seaweed species from Strangford Lough: compositional analyses of polysaccharides and bioassay of extracts using mung bean (Vigno mungo L.) and pak choi (Brassica rapa chinensis L.). J Appl Phycol 24, 1081–1091 (2012). https://doi.org/10.1007/s10811-011-9737-5

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  • DOI: https://doi.org/10.1007/s10811-011-9737-5

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