Abstract
The three color morphotypes of the red alga Kappaphycus alvarezii (brown, red and green) were cultured in Camranh Bay, Vietnam, using the fixed off-bottom monoline culture method to evaluate the growth rate, carrageenan yield, 3,6-anhydrogalactose, gel strength and lectin content. The brown morphotype was cultivated over a 12-month period; the red and green morphotypes were over a 6-month period. At the 60-day culture timepoint, the brown morphotype showed a higher growth rate (3.5–4.6% day−1) from September to February, and lower growth rate (1.6–2.8% day−1) from March to August. Significant (P < 0.05) differences in growth rate between culture months were found with the brown morphotype. High growth rates for the red (3.6–4.4% day−1) and green (3.7–4.2% day−1) morphotypes were obtained from September to February. The carrageenan yield, 3,6-anhydrogalactose and gel strength of the three morphotypes showed little variation, with the highest values obtained in November–December. At the 30-day sampling point, the brown morphotype had a higher lectin content (167–302 μg g−1 dry alga) from August to March and a lower lectin content (23–104 μg g−1 dry alga) from April to July. High lectin contents were recorded for the red (139–338 μg g−1 dry alga) and green (124–259 μg g−1 dry alga) morphotypes from September to February. This study shows that the different morphotypes of K. alvarezii can be grown in the tropical waters of the Camranh during the northeast monsoon, and part of the southwest monsoon, especially the brown morphotype, which can be grown during any season.
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References
Adnan H, Porse H (1987) Culture of Eucheuma cottonii and Eucheuma spinosum in Indonesia. Proc Int Seaweed Symp 12:355–358
Ask EI, Azanza RV (2002) Advances in cultivation technology of commercial eucheumatoid species: a review with suggestions for future research. Aquaculture 206:257–277
Ask EI, Batibasaga A, Zertuche-Gonzalez JA, De San M (2003) Three decades of Kappaphycus alvarezii (Rhodophyta) introduction to non-endemic locations. In: Chapman ARO, Anderson RJ, Vreeland VJ, Davison IR (eds) Proceedings of the 17th international seaweed symposium. Oxford University Press, Oxford, pp 49–57
Benevides NMB, Oliveira SRM, Holanda ML, Melo FR, Freitas ALP, Sampaio AH (1999) Seasonal variations in hemagglutinating activity and chemical composition of two red marine algae Gracilaria domigensis and Gelidium pusillum. R Bras Fisiol Veg 11:91–95
Bird KT, Chiles TC, Longley RE, Kendrick AF, Kinkema MD (1993) Agglutinins from marine macroalgae of the Southeastern United States. J Appl Phycol 5:212–213
Blunden G, Rogers DJ, Farnham WF (1975) Survey of British seaweeds for hemagglutinins. Lloydia 38:162–168
Boyd WC, Almodovar LR, Boyd LG (1966) Agglutinins in marine algae for human erythrocytes. Transfusion (Philadelphia) 6:82–83
Dawes CJ, Lluisma AO, Trono GC (1994) Laboratory and field growth studies of commercial strains of Eucheuma denticulatum and Kappaphycus alvarezii in the Philippines. J Appl Phycol 6:21–24
D’Elia CF, DeBoer JA (1978) Nutritional studies of two red algae: II. Kinetics of ammonium and nitrate uptake. J Phycol 14:266–272
Doty MS (1987) The production and use of Eucheuma. FAO Fisheries Technical Paper No. 281, Rome, pp 123–164
Fabregas J, Llovo J, Muñoz A (1985) Hemagglutinins in red seaweeds. Bot Mar 28:517–520
Freitas ALP, Teixeira DIA, Costa FHF, Farias WRL, Lobato ASC, Sampaio AH, Benevides NMB (1997) A new survey of Brazilian marine algae for agglutinins. J Appl Phycol 9:495–501
Glenn EP, Doty MS (1992) Water motion affects the growth rates of Kappaphycus alvarezii and related seaweeds. Aquaculture 108:233–246
Hayashi L, Paula EJD, Chow F (2007) Growth rate and carrageenan analyses in four strains of Kappaphycus alvarezii (Rhodophyta, Gigartinales) farmed in the subtropical waters of São Paulo State, Brazil. J Appl Phycol 19:393–399
Hellebust JA, Craige JS (1978) Handbook of phycological methods. Physiological and biochemical methods. Cambridge University Press, London, pp 110–131
Hori K, Miyazawa K, Ito K (1986) Preliminary characterization of agglutinins from seven marine algal species. Bull Jpn Soc Sci Fish 52(2):323–331
Hori K, Oiwa C, Miyazawa K, Ito K (1988) Evidence for wide distribution of agglutinins in marine algae. Bot Mar 31:133–138
Hurtado-Ponce AQ (1992) Cage culture of Kappaphycus alvareziivar. tambalang (Gigartinales, Rhodophyceae). J Appl Phycol 4:311–313
Hurtado-Ponce AQ (1995) Carrageenan properties and proximate composition of three morphotypes of Kappaphycus alvarezii Doty (Gigartinales, Rhodophyta). Bot Mar 38:215–219
Hurtado-Ponce AQ, Agbayani RF, Sanares R, Castro-Mallare MTR (2001) The seasonality and economic feasibility of cultivating Kappaphycus alvarezii in Panagatan Cays, Caluya, Antique Philippines. Aquaculture 199:295–310
Kawakubo A, Makino H, Ohnishi J, Hirohara H, Hori K (1999) Occurrence of highly yielded lectins homologous within the genus Eucheuma. J Appl Phycol 11:149–156
Luxton DM, Robertson M, Kindley MJ (1987) Farming Eucheuma in the South Pacific islands of Fiji. Proc Int Seaweed Symp 12:359–362
Mollion J, Braud JP (1993) A Eucheuma (Solieriaceae, Rhodophyta) cultivation test on the south-west coast of Madagascar. Hydrobiologia 260/261:373–378
Munoz J, Freile-Pelegrín Y, Robledo D (2004) Mariculture of Kappaphycus alvarezii (Rhodophyta, Solieriaceae) color strains in tropical waters of Yucatán, México. Aquaculture 239:161–177
Ohno M, Largo DB, Ikumoto T (1994) Growth rate, carrageenan yield and gel properties of cultured kappa-carrageenan producing red alga Kappaphycus alvarezii (Doty) Doty in the subtropical waters of Shikoku, Japan. J Appl Phycol 6:1–5
Ohno M, Nang HQ, Hirase S (1996) Cultivation and carrageenan yield and quality of Kappaphycus alvarezii in the waters of Vietnam. J Appl Phycol 8:431–437
Parsons TR, Maita Y, Lalli CM (1984) A manual of chemical and biological methods of seawater analysis. Pergamon, Oxford
Sampaio AH, Rogers DJ, Barwell CJ, Farnham WF (1996) Seasonal variation in production of lectins by Ulva lactuca. In: Van Drieesche E, Rouge P, Beeckmans S, Bog-Hansen TC (eds) Lectins: Biology, biochemistry clinical biochemistry. Textop Hellerup, Denmark, pp 101–106
Santos GA (1989) Carrageenans of species of Eucheuma J. Agardh and Kappaphycus Doty (Solieriaceae, Rhodophyta). Aquat Bot 36:55–67
Sharon N, Lis H (2003) Lectins, (2nd edn). Kluwer, The Netherlands
Takahashi Y, Katagiri S (1987) Seasonal variation of the hemagglutination activity in the red alga Gracilaria verrucosa. Nippon Suisan Gakkaishi 53:2133–2137
Trono GC, Ohno M (1989) Seasonality in the biomass production of Eucheuma strains in northern Bohol, Philippines. In: Umezaki I (ed) Scientific survey of marine algae and their resources in the Philippine Islands. Monbushio international scientific research program, Japan, pp 71–80
Trono GC (1992) Eucheuma and Kappaphycus: taxonomy and cultivation. Bull Mar Sci Fish Kochi Univ 12:51–65
Wakibia JG, Bolton JJ, Keats DW, Raitt LM (2006) Factors influencing the growth rates of three commercial eucheumoids at coastal sites in southern Kenya. J Appl Phycol 18:565–573
Yaphe W, Arsenault GP (1965) Improved resorcinol reagent for the determination of fructose, and of 3,6-anhydrogalactose in polysaccharides. Anal Biochem 13:143–148
Acknowledgments
We are grateful to the staff of Nhatrang Institute of Technology Research and Application, Vietnam for collection of algal samples, and to colleagues at the Laboratory of Marine Bioresource Chemistry, Hiroshima University for providing expert technical assistance. This work was supported by JSPS- RONPAKU Program, Japan and Project of Ministry of Fishery, Vietnam: “Investigation – Scheme and put forwarding solutions for stable cultivation of Kappaphycus alvarezii (Doty)”.
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Hung, L.D., Hori, K., Nang, H.Q. et al. Seasonal changes in growth rate, carrageenan yield and lectin content in the red alga Kappaphycus alvarezii cultivated in Camranh Bay, Vietnam. J Appl Phycol 21, 265–272 (2009). https://doi.org/10.1007/s10811-008-9360-2
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DOI: https://doi.org/10.1007/s10811-008-9360-2