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Modified technique of internal limiting membrane staining in idiopathic macular hole surgery

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Abstract

Purpose

To describe a new method of ILM staining with TB under perfluorocarbon in cases of full thickness idiopathic macular hole using the inverted ILM flap technique.

Methods

This study was a prospective interventional case series that included 42 eyes of 42 patients who had full thickness idiopathic macular hole with a minimum diameter more than 400 µm. Patients consecutively underwent vitrectomy with inverted ILM flap technique using the modified ILM staining method.

Results

Anatomic success was achieved in 40 patients (95.2%). The other two patients had flat-open macular holes with bare RPE (foveal defect of neurosensory retina). Among the 40 eyes with closed holes, 25 eyes were of the U-type closure (normal foveal contour) and 15 eyes were of the V-type closure (steep foveal contour). These 40 eyes remained closed during the 6 months follow-up period.

Conclusion

The modified technique of ILM staining using TB under PFCL is safe and effective in cases of idiopathic macular hole combined with the inverted ILM flap technique.

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References

  1. Eckardt C, Eckardt U, Groos S, Luciano L, Reale E (1997) Removal of the internal limiting membrane in macular holes. Clinical and morphological findings. Ophthalmologe 94:545–551

    Article  CAS  PubMed  Google Scholar 

  2. Rodrigues EB, Meyer CH, Farah ME, Kroll P (2005) Intravitreal staining of the internal limiting membrane using indocyanine green in the treatment of macular holes. Ophthalmologica 219:251–262

    Article  PubMed  Google Scholar 

  3. Mester V, Kuhn F (2000) Internal limiting membrane removal in the management of full-thickness macular holes. Am J Ophthalmol 129:769–777

    Article  CAS  PubMed  Google Scholar 

  4. Abdelkader E, Lois N (2008) Internal limiting membrane peeling in vitreo-retinal surgery. Surv Ophthalmol 53:368–396

    Article  PubMed  Google Scholar 

  5. Farah ME, Maia M, Rodrigues EB (2009) Dyes in ocular surgery: principles for use in chromovitrectomy. Am J Ophthalmol 148:332–340

    Article  PubMed  Google Scholar 

  6. Lee KL, Dean S, Guest S (2005) A comparison of outcomes after indocyanine green and trypan blue assisted internal limiting membrane peeling during macular hole surgery. Br J Ophthalmol 89:420–424

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Beutel J, Dahmen G, Ziegler A, Hoerauf H (2007) Internal limiting membrane peeling with indocyanine green or trypan blue in macular hole surgery: a randomized trial. Arch Ophthalmol 125:326–332

    Article  PubMed  Google Scholar 

  8. Maia M, Haller JA, Pieramici DJ et al (2004) Retinal pigment epithelial abnormalities after internal limiting membrane peeling guided by indocyanine green staining. Retina 24:157–160

    Article  PubMed  Google Scholar 

  9. Uemoto R, Yamamoto S, Takeuchi S (2005) Changes in retinal pigment epithelium after indocyanine green-assisted internal limiting lamina peeling during macular hole surgery. Am J Ophthalmol 140:752–755

    Article  PubMed  Google Scholar 

  10. Michalewska Z, Michalewski J, Adelman RA, Nawrocki J (2010) Inverted internal limiting membrane flap technique for large macular holes. Ophthalmology 117:2018–2025

    Article  PubMed  Google Scholar 

  11. Rodrigues EB, Meyer CH, Kroll P (2005) Chromovitrectomy: a new field in vitreoretinal surgery. Graefes Arch Clin Exp Ophthalmol 243:291–293

    Article  PubMed  Google Scholar 

  12. Burk SE, Da Mata AP, Snyder ME et al (2000) Indocyanine green-assisted peeling of the retinal internal limiting membrane. Ophthalmology 107:2010–2014

    Article  CAS  PubMed  Google Scholar 

  13. Veckeneer M, van Overdam K, Monzer J et al (2001) Ocular toxicity study of trypan blue injected into the vitreous cavity of rabbit eyes. Graefes Arch Clin Exp Ophthalmol 239:698–704

    Article  CAS  PubMed  Google Scholar 

  14. Melles GR, de Waard PW, Pameyer JH, Houdijn BW (1999) Trypan blue capsule staining to visualize the capsulorhexis in cataract surgery. J Cataract Refract Surg 25:7–9

    Article  CAS  PubMed  Google Scholar 

  15. Feron EJ, Veckeneer M, Parys-Van GR, Van LA, Melles GR, Stalmans P (2002) Trypan blue staining of epiretinal membranes in proliferative vitreoretinopathy. Arch Ophthalmol 120:141–144

    Article  PubMed  Google Scholar 

  16. Gale JS, Proulx AA, Gonder JR, Mao AJ, Hutnik CM (2004) Comparison of the in vitro toxicity of indocyanine green to that of Trypan blue in human retinal pigment epithelium cell cultures. Am J Ophthalmol 138:64–69

    Article  CAS  PubMed  Google Scholar 

  17. Penha FM, Maia M, Eid FM, Principe AH, Freymuller EH, Maia A et al (2007) Effects of subretinal injections of indocyanine green, trypan blue, and glucose in rabbit eyes. Ophthalmology 114:899–908

    Article  PubMed  Google Scholar 

  18. Kwok AK, Yeung CK, Lai TY, Chan KP, Pang CP (2004) Effects of trypan blue on cell viability and gene expression in human retinal pigment epithelial cells. Br J Ophthalmol 88:1590–1594

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Stalmans P, Van Aken EH, Melles G, Veckeneer M, Feron EJ, Stalmans I (2003) Trypan blue not toxic for retinal pigment epithelium in vitro. Am J Ophthalmol 135:234–236

    Article  CAS  PubMed  Google Scholar 

  20. Hirasawa H, Yanagi Y, Tamaki Y, Inoue Y, Kadonosono K (2007) Indocyanine green and trypan blue: intracellular uptake and extracellular binding by human retinal pigment epithelial cells. Retina 27:375–378

    Article  PubMed  Google Scholar 

  21. Li K, Wong D, Hiscott P et al (2003) Trypan blue staining of internal limiting membrane and epiretinal membrane during vitrectomy: visual results and histopathological findings. Br J Ophthalmol 87(2):216–219

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Rodrigues EB, Maia M, Meyer CH, Penha FM, Dib E, Farah ME (2007) Vital dyes for chromovitrectomy. Curr Opin Ophthalmol 18:179–187

    Article  PubMed  Google Scholar 

  23. Maia M, Penha F, Rodrigues EB et al (2007) Effects of subretinal injection of patent blue and trypan blue in rabbits. Curr Eye Res 32:309–317

    Article  PubMed  Google Scholar 

  24. Negi A, Marmor MF (1984) Effects of subretinal and systemic osmolality on the rate of subretinal fluid resorption. Invest Ophthalmol Vis Sci 25:616–620

    CAS  PubMed  Google Scholar 

  25. Perrier M, Sebag M (2003) Trypan blue-assisted peeling of the internal limiting membrane during macular hole surgery. Am J Ophthalmol 135:903–905

    Article  PubMed  Google Scholar 

  26. Oberstein SYL, Mura M, Tan SH, de Smet MD (2007) Heavy trypan blue staining of epiretinal membranes: an alternative to infracyanine green. Br J Ophthalmol 91:955–957

    Article  PubMed Central  Google Scholar 

  27. Oberstein SYL, de Smet MD (2010) Use of heavy trypan blue in macular hole surgery. Eye 24:1177–1181

    Article  Google Scholar 

  28. Chang S (1987) Low viscosity liquid fluorochemicals in vitreous surgery. Am J Ophthalmol 103:38–43

    Article  CAS  PubMed  Google Scholar 

  29. Facino M, Mochi B, Lai S, Terrile R (2004) A simple way to prevent indocyanine green from entering the subretinal space during vitrectomy for retinal detachment due to myopic macular hole. Eur J Ophthalmol 14(3):269–271

    Article  CAS  PubMed  Google Scholar 

  30. Olson JL, On AV, Mandava N (2005) Protecting the retinal pigment epithelium during macular hole surgery. Clin Exp Ophthalmol 33(6):576–577

    Article  PubMed  Google Scholar 

  31. Scupola A, Giammaria D, Tiberti AC, Balestrazzi E (2006) Use of perfluorocarbon liquid to prevent contact between indocyanine green and retinal pigment epithelium during surgery for idiopathic macular hole. Retina 26(2):236–237

    Article  PubMed  Google Scholar 

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Authors and Affiliations

Authors

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Correspondence to Mahmoud Alaa Abouhussein.

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Conflict of interest

Both authors declare that they have no conflict of interest.

Research involving human participants

Alexandria University Ethical Committee approval was obtained, research was adherent to Declaration of Helsinki.

Informed consent

Informed consent was obtained from all patients.

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Video showing the new technique of ILM staining (MP4 10022 kb)

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El-Baha, S.M., Abouhussein, M.A. Modified technique of internal limiting membrane staining in idiopathic macular hole surgery. Int Ophthalmol 39, 557–562 (2019). https://doi.org/10.1007/s10792-018-0842-1

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  • DOI: https://doi.org/10.1007/s10792-018-0842-1

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