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Comparison of serum thiol–disulphide homeostasis and total antioxidant–oxidant levels between exudative age-related macular degeneration patients and healthy subjects

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Abstract

Purpose

The purpose of the study was to calculate serum total oxidant status (TOS), total antioxidant status (TAS), and dynamic thiol–disulphide (T-D) homeostasis in patients with age-related macular degeneration (AMD), and compare the results with healthy individuals.

Methods

Thirty-three exudative AMD patients and 33 healthy controls were included in this case–control study. Participants’ serum TAS and TOS levels were measured. In addition, total thiol (TT), native thiol (NT), and disulphide (DS) concentrations were assessed using a novel automated method of measurement.

Results

In comparison with the control group, serum TAS, TT, and NT levels were found to be significantly lower (p < 0.0001, p = 0.004, p = 0.003, respectively) and TOS levels were detected higher (p = 0.032) in AMD patients. Serum DS levels were elevated in the AMD patient group, but the difference was not statistically significant (p = 0.219). DS/TT and DS/NT ratios were significantly higher (p = 0.012, p = 0.013, respectively) in AMD patients. A positive correlation was found between TT and NT (p < 0.0001) in AMD group.

Conclusions

Serum TOS levels are higher, TAS levels are lower, and the T-D balance is shifted to the DS bond side in AMD patients. These results suggest that increased oxidative stress and decreased antioxidant levels may play a role in AMD progression. Further studies are needed to confirm the pathophysiologic role of T-D homeostasis in AMD.

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Acknowledgments

No specific financial support was available for this study.

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Authors and Affiliations

  1. Department of Ophthalmology, Bezmialem University School of Medicine, Zümrütevler Mh. Yol-6 Sk. No:2 Seyrantepe Konutları A4/52 Maltepe, Istanbul, Turkey

    Ahmet Elbay, Julide Canan Umurhan Akkan, Arif Koytak & Hakan Ozdemir

  2. Department of Biochemistry, Bezmialem University School of Medicine, Istanbul, Turkey

    Omer Faruk Ozer

  3. Department of Ophthalmology, Istanbul Medeniyet University School of Medicine, Istanbul, Turkey

    Ugur Celik

  4. Department of Ophthalmology, Umraniye Training and Research Hospital, Istanbul, Turkey

    Işıl Kutlutürk

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  1. Ahmet Elbay
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  2. Omer Faruk Ozer
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  3. Julide Canan Umurhan Akkan
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  4. Ugur Celik
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  5. Işıl Kutlutürk
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Correspondence to Ahmet Elbay.

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Conflict of interest

None of the authors have any potential conflict of interest to disclose. These data have not been previously published. AE, OFO, and JCUA were involved in design and conduct of the study ; AE, JCUA, and AK were involved in data collection ; AE, UC, and IK were involved in analyses of the data; AE was involved in writing the article (AE); OFO, IK, AK, and HO were involved in review of the study .

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Elbay, A., Ozer, O.F., Akkan, J.C.U. et al. Comparison of serum thiol–disulphide homeostasis and total antioxidant–oxidant levels between exudative age-related macular degeneration patients and healthy subjects. Int Ophthalmol 37, 1095–1101 (2017). https://doi.org/10.1007/s10792-016-0367-4

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  • DOI: https://doi.org/10.1007/s10792-016-0367-4

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