Abstract
Pulmonary fibrosis is a result of an abnormal wound healing in lung tissue triggered by an excessive accumulation of extracellular matrix proteins, loss of tissue elasticity, and debit of ventilatory function. NKT cells are a major source of Th1 and Th2 cytokines and may be crucial in the polarization of M1/M2 macrophages in pulmonary fibrogenesis. Although there appears to be constant scientific progress in that field, pulmonary fibrosis still exhibits no current cure. From these facts, we hypothesized that NKT cells could influence the development of pulmonary fibrosis via modulation of macrophage activation. Wild type (WT) and NKT type I cell-deficient mice (Jα18−/−) were subjected to the protocol of bleomycin-induced pulmonary fibrosis with or without treatment with NKT cell agonists α-galactosylceramide and sulfatide. The participation of different cell populations, collagen deposition, and protein levels of different cytokines involved in inflammation and fibrosis was evaluated. The results indicate a benign role of NKT cells in Jα18−/− mice and in wild-type α-galactosylceramide–sulfatide-treated groups. These animals presented lower levels of collagen deposition, fibrogenic molecules such as TGF-β and vimentin and improved survival rates. In contrast, WT mice developed a Th2-driven response augmenting IL-4, 5, and 13 protein synthesis and increased collagen deposition. Furthermore, the arginase-1 metabolic pathway was downregulated in wild-type NKT-activated and knockout mice indicating lower activity of M2 macrophages in lung tissue. Hence, our data suggest that NKT cells play a protective role in this experimental model by down modulating the Th2 milieu, inhibiting M2 polarization and finally preventing fibrosis.
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References
Allen JE, Sutherland TE (2014) Host protective roles of type 2 immunity: parasite killing and tissue repair, flip sides of the same coin. Semin Immunol 26:329–340
Ambrosino E, Terabe M, Halder RC et al (2007) Cross-regulation between type I and type II NKT cells in regulating tumor immunity: a new immunoregulatory axis. J Immunol 179:5126–5136. doi:10.4049/jimmunol.179.8.5126
American Thoracic Society (2000) Idiopathic pulmonary fibrosis: diagnosis and treatment. Am J Respir Crit Care Med 161:646–664
Arrenberg P, Maricic I, Kumar V (2011) Sulfatide-mediated activation of type II natural killer T cells prevents hepatic ischemic reperfusion injury in mice. Gastroenterology 140:646–655. doi:10.1053/j.gastro.2010.10.003
Aspeslagh S, Li Y, Yu ED et al (2011) Galactose-modified iNKT cell agonists stabilized by an induced fit of CD1d prevent tumour metastasis. EMBO J 30:2294–2305. doi:10.1038/emboj.2011.145
Berzofsky JA, Terabe M (2009) The contrasting roles of NKT cells in tumor immunity. Curr Mol Med 9:667–672
Biernacka A, Dobaczewski M, Frangogiannis NG (2011) TGF-β signaling in fibrosis. Growth Factors 29:196–202. doi:10.3109/08977194.2011.595714
Burdin N, Brossay L, Kronenberg M (1999) Immunization with alpha-galactosylceramide polarizes CD1-reactive NKT cells towards Th2 cytokine synthesis. Eur J Immunol 29:2014–2025
Carreño LJ, Kharkwal SS, Porcelli SA (2014) Optimizing NKT cell ligands as vaccine adjuvants. Immunotherapy 6:309–320. doi:10.2217/imt.13.175
Cassetta L, Cassol E, Poli G (2011) Macrophage polarization in health and disease. Sci World J 11:2391–2402. doi:10.1100/2011/213962
Chua F, Gauldie J, Laurent GJ (2005) Pulmonary fibrosis: searching for model answers. Am J Respir Cell Mol Biol 33:9–13
Coker RK, Laurent GJ (1998) Pulmonary fibrosis: cytokines in the balance. Eur Respir J 11:1218–1221
Cui J, Shin T, Kawano T et al (1997) Requirement for Valpha14 NKT cells in IL-12-mediated rejection of tumors. Science 278:1623–1626. doi:10.1126/science.278.5343.1623
East JE, Kennedy AJ, Webb TJ (2014) Raising the roof: the preferential pharmacological stimulation of Th1 and Th2 responses mediated by NKT cells. Med Res Rev 34:45–76. doi:10.1002/med.21276
Fuss IJ, Heller F, Boirivant M et al (2004) Nonclassical CD1d-restricted NK T cells that produce IL-13 characterize an atypical Th2 response in ulcerative colitis. J Clin Invest 113:1490–1497. doi:10.1172/JCI19836
Gao B, Radaeva S (2013) Natural killer and natural killer T cells in liver fibrosis. Biochim Biophys Acta Mol Basis Dis 1832:1061–1069. doi:10.1016/j.bbadis.2012.09.008
Gharaee-Kermani M, McGarry B, Lukacs N et al (1998) The role of IL-5 in bleomycin-induced pulmonary fibrosis. J Leukoc Biol 64:657–666
Gharaee-Kermani M, Nozaki Y, Hatano K, Phan SH (2001) Lung interleukin-4 gene expression in a murine model of bleomycin-induced pulmonary fibrosis. Cytokine 15:138–147. doi:10.1006/cyto.2001.0903
Gordon S, Martinez FO (2010) Alternative activation of macrophages: mechanism and functions. Immunity 32:593–604
Halder RC, Aguilera C, Maricic I, Kumar V (2007) Type II NKT cell-mediated anergy induction in type I NKT cells prevents inflammatory liver disease. J Clin Invest 117:2302–2312. doi:10.1172/JCI31602
Huang LS, Natarajan V (2015) Sphingolipids in pulmonary fibrosis. Adv Biol Regul 57:55–63. doi:10.1016/j.jbior.2014.09.008
Jahng A, Maricic I, Aguilera C et al (2004) Prevention of autoimmunity by targeting a distinct, noninvariant CD1d-reactive T cell population reactive to sulfatide. J Exp Med 199:947–957. doi:10.1084/jem.20031389
Kim JH, Kim HY, Kim S et al (2005) Natural killer T (NKT) cells attenuate bleomycin-induced pulmonary fibrosis by producing interferon-gamma. Am J Pathol 167:1231–1241
Kimura T, Ishii Y, Morishima Y et al (2004) Treatment with alpha-galactosylceramide attenuates the development of bleomycin-induced pulmonary fibrosis. J Immunol 172:5782–5789
Kumar V (2013) NKT-cell subsets: promoters and protectors in inflammatory liver disease. J Hepatol 59:618–620. doi:10.1016/j.jhep.2013.02.032
Lee CG, Homer RJ, Zhu Z et al (2001) Interleukin-13 induces tissue fibrosis by selectively stimulating and activating transforming growth factor beta(1). J Exp Med 194:809–821. doi:10.1084/jem.194.6.809
Liao C-M, Zimmer MI, Wang C-R (2013) The functions of type I and type II natural killer T cells in inflammatory bowel diseases. Inflamm Bowel Dis 19:1330–1338. doi:10.1097/MIB.0b013e318280b1e3
Maier E, Duschl A, Horejs-Hoeck J (2012) STAT6-dependent and -independent mechanisms in Th2 polarization. Eur J Immunol 42:2827–2833. doi:10.1002/eji.201242433
Marrero I, Ware R, Kumar V (2015) Type II NKT cells in inflammation, autoimmunity, microbial immunity, and cancer. Front Immunol 6:316. doi:10.3389/fimmu.2015.00316
Mitra A, Satelli A, Yan J et al (2014) IL-30 (IL27p28) attenuates liver fibrosis through inducing NKG2D-rae1 interaction between NKT and activated hepatic stellate cells in mice. Hepatology 60:2027–2039. doi:10.1002/hep.27392
Miyamoto K, Miyake S, Yamamura T (2001) A synthetic glycolipid prevents autoimmune encephalomyelitis by inducing TH2 bias of natural killer T cells. Nature 413:531–534. doi:10.1038/35097097
Moreira AP, Hogaboam CM (2011) Macrophages in allergic asthma: fine-tuning their pro- and anti-inflammatory actions for disease resolution. J Interferon Cytokine Res 31:485–491. doi:10.1089/jir.2011.0027
Murray LA, Rosada R, Moreira AP et al (2010) Serum amyloid P therapeutically attenuates murine bleomycin-induced pulmonary fibrosis via its effects on macrophages. PLoS One. doi:10.1371/journal.pone.0009683
Nadas J, Li C, Wang PG (2009) Computational structure activity relationship studies on the CD1d/glycolipid/TCR complex using AMBER and AUTODOCK. J Chem Inf Model 49:410–423. doi:10.1021/ci8002705
Novak ML, Koh TJ (2013) Macrophage phenotypes during tissue repair. J Leukoc Biol 93:875–881. doi:10.1189/jlb.1012512
Paun A, Bergeron M-E, Haston CK (2014) NKT deficient mice are not spared lung disease after exposure to thoracic radiotherapy. Radiat Res 181:369–375. doi:10.1667/RR13581.1
Pechkovsky DV, Prasse A, Kollert F et al (2010) Alternatively activated alveolar macrophages in pulmonary fibrosis-mediator production and intracellular signal transduction. Clin Immunol 137:89–101. doi:10.1016/j.clim.2010.06.017
Ricardo SD, Van Goor H, Eddy AA (2008) Macrophage diversity in renal injury and repair. J Clin Invest 118:3522–3530
Schmieg J, Yang G, Franck RW, Tsuji M (2003) Superior protection against malaria and melanoma metastases by a C-glycoside analogue of the natural killer T cell ligand α-galactosylceramide. J Exp Med 198:1631–1641. doi:10.1084/jem.20031192
Smyth MJ, Godfrey DI (2000) NKT cells and tumor immunity—a double-edged sword. Nat Immunol 1:459–460. doi:10.1038/82698
Srour N, Thébaud B (2015) Mesenchymal stromal cells in animal bleomycin pulmonary fibrosis models: a systematic review. Stem Cells Transl Med 4:1500–1510. doi:10.5966/sctm.2015-0121
Stahl M, Schupp J, Jäger B et al (2013) Lung collagens perpetuate pulmonary fibrosis via CD204 and M2 macrophage activation. PLoS One. doi:10.1371/journal.pone.0081382
Strieter RM, Keane MP (2004) Innate immunity dictates cytokine polarization relevant to the development of pulmonary fibrosis. J Clin Invest 114:165–168
Syn W-K, Agboola KM, Swiderska M et al (2012) NKT-associated hedgehog and osteopontin drive fibrogenesis in non-alcoholic fatty liver disease. Gut 61:1323–1329. doi:10.1136/gutjnl-2011-301857
Tanaka S, Tsukada J, Suzuki W et al (2006) The interleukin-4 enhancer CNS-2 is regulated by notch signals and controls initial expression in NKT cells and memory-type CD4 T cells. Immunity 24:689–701. doi:10.1016/j.immuni.2006.04.009
Terabe M, Berzofsky JA (2008) The role of NKT cells in tumor immunity. Adv Cancer Res 101:277–348. doi:10.1016/S0065-230X(08)00408-9
Trujillo G, O’Connor EC, Kunkel SL, Hogaboam CM (2008) A novel mechanism for CCR4 in the regulation of macrophage activation in bleomycin-induced pulmonary fibrosis. Am J Pathol 172:1209–1221. doi:10.2353/ajpath.2008.070832
Van Kaer L (2004) Regulation of immune responses by CD1d-restricted natural killer T cells. Immunol Res 30:139–153. doi:10.1385/IR:30:2:139
Van Kaer L, Parekh VV, Wu L (2011) Invariant natural killer T cells: bridging innate and adaptive immunity. Cell Tissue Res 343:43–55
Varin A, Gordon S (2009) Alternative activation of macrophages: immune function and cellular biology. Immunobiology 214:630–641. doi:10.1016/j.imbio.2008.11.009
Wynn Ta (2004) Fibrotic disease and the T(H)1/T(H)2 paradigm. Nat Rev Immunol 4:583–594. doi:10.1038/nri1412
Xiang J, Cheng S, Feng T et al (2016) Neotuberostemonine attenuates bleomycin-induced pulmonary fibrosis by suppressing the recruitment and activation of macrophages. Int Immunopharmacol 36:158–164. doi:10.1016/j.intimp.2016.04.016
Yang SH, Lee JP, Jang HR et al (2011) Sulfatide-reactive natural killer T cells abrogate ischemia-reperfusion injury. J Am Soc Nephrol 22:1305–1314. doi:10.1681/ASN.2010080815
Zhang K, Phan SH (1996) Cytokines and pulmonary fibrosis. Biol Signals 5:232–239
Zhang K, Gharaee-Kermani M, McGarry B, Phan SH (1994) In situ hybridization analysis of rat lung alpha 1(I) and alpha 2(I) collagen gene expression in pulmonary fibrosis induced by endotracheal bleomycin injection. Lab Invest 70:192–202
Acknowledgements
We would like to thank all who contributed to the accomplishment of this work. We would like to thank Masaru Taniguchi at the RIKEN Research Center for Allergy and Immunology (Japan) for Jα18KO. In addition, the authors are grateful to Bernardo Paulo Albe for helping set up the histology slides and Andrea Glatt, Renata Ricardi, Claudia Silva Cunha, and Marcílio Tablas Neto for technical assistance. This work was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Complex Fluids INCT). The funders had no role in study design, data collection, and analysis; decision to publish; or preparation of the manuscript.
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Grabarz, F., Aguiar, C.F., Correa-Costa, M. et al. Protective role of NKT cells and macrophage M2-driven phenotype in bleomycin-induced pulmonary fibrosis. Inflammopharmacol 26, 491–504 (2018). https://doi.org/10.1007/s10787-017-0383-7
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DOI: https://doi.org/10.1007/s10787-017-0383-7