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Localized delivery of curcumin from injectable gelatin/Thai silk fibroin microspheres for anti-inflammatory treatment of osteoarthritis in a rat model

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Abstract

The previously developed gelatin/silk fibroin microspheres were loaded with curcumin and applied for anti-inflammatory treatment in monosodium iodoacetate (MIA)-induced osteoarthritis (OA) in a rat model. The MIA-induced OA rats received a single intra-articular injection with gelatin or gelatin/silk fibroin (30/70) microspheres encapsulating curcumin. The therapeutic effects of treatment groups [concentration of interleukin-6 (IL-6) in blood serum, radiographic and the histological grading on articular joint] were compared with those of normal saline treated OA and normal rats. The result showed that both microsphere groups reduced the level of IL-6 in serum after 1 week of treatment. The gelatin/silk fibroin (30/70) microspheres encapsulating curcumin delayed the cellular destruction in articular joint and synovial tissue after 8 weeks. The radiographic and histological gradings on articular cartilage lesion and synovial tissue change of rats treated with gelatin/silk fibroin (30/70) microspheres encapsulating curcumin were close to those of the normal rats. It was explained that the slow-degrading gelatin/silk fibroin (30/70) microspheres released curcumin for extended period and showed a prolonged anti-inflammatory effect, compared to the fast-degrading gelatin microspheres. This delivery system of curcumin was suggested to be applied for localized treatment of anti-inflammatory in OA with minimal invasion.

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Acknowledgements

This research was supported by the Ratchadaphiseksomphot Endowment Fund Part of the "Research Grant for New Scholar CU Researcher's Project (RGN_2558_019_02_21) and Faculty of Engineering, Chulalongkorn University.

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Authors and Affiliations

  1. Biomedical Engineering Program, Faculty of Engineering, Chulalongkorn University, PhayaThai Road, Bangkok, 10330, Thailand

    Juthamas Ratanavaraporn

  2. Department of Veterinary Surgery, Faculty of Veterinary Science, Chulalongkorn University, PhayaThai Road, Bangkok, 10330, Thailand

    Kumpanart Soontornvipart

  3. Department of Medical Services, Institute of Pathology, Ministry of Public Health, Yothi Road, Bangkok, 10400, Thailand

    Somruetai Shuangshoti

  4. Department of Pathology and Chulalongkorn GenePRO Center, Faculty of Medicine, Chulalongkorn University, Rama IV Road, Bangkok, 10330, Thailand

    Shanop Shuangshoti

  5. Department of Chemical Engineering, Faculty of Engineering, Chulalongkorn University, PhayaThai Road, Bangkok, 10330, Thailand

    Siriporn Damrongsakkul

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  1. Juthamas Ratanavaraporn
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  2. Kumpanart Soontornvipart
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  3. Somruetai Shuangshoti
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  4. Shanop Shuangshoti
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  5. Siriporn Damrongsakkul
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Correspondence to Siriporn Damrongsakkul.

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Ratanavaraporn, J., Soontornvipart, K., Shuangshoti, S. et al. Localized delivery of curcumin from injectable gelatin/Thai silk fibroin microspheres for anti-inflammatory treatment of osteoarthritis in a rat model. Inflammopharmacol 25, 211–221 (2017). https://doi.org/10.1007/s10787-017-0318-3

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  • DOI: https://doi.org/10.1007/s10787-017-0318-3

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