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Application of THz Time-Domain Spectroscopy to Diagnose Gastric Cancer Tissues in Surgical Resected Specimens

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Abstract

Based on tissues from 34 patients, we investigated the performance of terahertz (THz) time-domain spectroscopy (TDS) to diagnose gastric tissues in surgical resected specimens. The results indicated that THz absorption spectra not only can clearly distinguish cancer tissues from normal tissues without hematoxylin-eosin staining (H&E staining) but also can identify carcinoma in situ and cancer tissues. Using THz-TDS to directly diagnose gastric cancer was also demonstrated with another 40 samples. Comparison of the data with the results of pathological examination indicated that the sensitivity and specificity were both 100%. Due to the ability to perform quantitative analysis and enable follow-up staining and traditional histopathological analysis, our study indicated considerable potential of THz-TDS for future automation, which is critical for the rapid and thorough prescreening of gastric cancer pathological examinations.

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References

  1. L. Yang, R. Zheng, N. Wang, Y. Yuan, S. Liu, H. Li, S. Zhang, H. Zeng, and W. Chen, “Incidence and mortality of stomach cancer in China, 2014,” Chinese J. Cancer Res. 30(3), 291–298 (2018).

    Article  Google Scholar 

  2. W. Chen, R. Zheng, P. D. Baade, S. Zhang, H. Zeng, F. Bray, A. Jemal, X. Q. Yu, and J. He, “Cancer statistics in China, 2015,” CA. Cancer J. Clin. 66(2), 115–132 (2016).

    Article  Google Scholar 

  3. S. Luo, C. Chen, H. Mao, and S. Jin, “Discrimination of premalignant lesions and cancer tissues from normal gastric tissues using Raman spectroscopy,” J. Biomed. Opt. 18(6), 067004:1-8 (2013).

    Article  Google Scholar 

  4. S. K. Teh, W. Zheng, K. Y. Ho, M. Teh, K. G. Yeoh, and Z. Huang, “Diagnostic potential of near-infrared Raman spectroscopy in the stomach: Differentiating dysplasia from normal tissue,” Brit. J. Cancer 98(2), 457–465 (2008).

    Article  Google Scholar 

  5. Q. Li, W. Wang, X. Ling, and J. G. Wu, “Detection of gastric cancer with fourier transform infrared spectroscopy and support vector machine classification,” Biomed Res. Int. 2013(9), 4–7 (2013).

    Google Scholar 

  6. W. S. Yi, D. S. Cui, Z. Li, L. L. Wu, A. G. Shen, and J. M. Hu, “Gastric cancer differentiation using Fourier transform near-infrared spectroscopy with unsupervised pattern recognition,” Spectrochim. Acta. A. 101, 127–131 (2013).

    Article  Google Scholar 

  7. Y. Bin Ji, C. H. Park, H. Kim, S.-H. Kim, G. M. Lee, S. K. Noh, T.-I. Jeon, J.-H. Son, Y.-M. Huh, S. Haam, S. J. Oh, S. K. Lee, and J.-S. Suh, “Feasibility of terahertz reflectometry for discrimination of human early gastric cancers,” Biomed. Opt. Express 6(4), 1398–1406 (2015).

    Article  Google Scholar 

  8. D. Hou, X. Li, J. Cai, Y. Ma, X. Kang, P. Huang, and G. Zhang, “Terahertz spectroscopic investigation of human gastric normal and tumor tissues,” Phys. Med. Biol. 59(18), 5423–5440 (2014).

    Article  Google Scholar 

  9. H. A. Kashanian, H. B. Ghaffary, and N. C. Bagherzadeh, “Gastric cancer diagnosis using terahertz imaging,” Majlesi J. Multimed. Process. 4(4), 1–7 (2015).

    Google Scholar 

  10. Y. Cao, P. Huang, X. Li, W. Ge, D. Hou and G. Zhang, “Terahertz spectral unmixing based method for identifying gastric cancer,” Phys. Med. Biol. 63(3), 035016 (2018)

    Article  Google Scholar 

  11. F. Wahaia, I. Kasalynas, D. Seliuta, G. Molis, A. Urbanowicz, C. D. Carvalho Silva, F. Carneiro, G. Valusis, and P. L. Granja, “Terahertz spectroscopy for the study of paraffin-embedded gastric cancer samples,” Journal of Molecular Structure, 1079, 391–395 (2015).

    Article  Google Scholar 

  12. E. Berry, G. C. Walker, A. J. Fitzgerald, N. N. Zinov’ev, M. Chamberlain, S. W. Smye, R. E. Miles, and M. A. Smith, “Do in vivo terahertz imaging systems comply with safety guidelines?” J. Laser Appl. 15(3), 192–198 (2003).

    Article  Google Scholar 

  13. K. H. Ng, “Non-ionizing radiations - sources, biological effects, emissions and exposures,” in Proceedings of the International Conference on Non-Ionizing Radiation at UNITEN (ICNIR2003) Electromagnetic Fields and Our Health (Selangor, Malaysia, 2003), pp. 1–16.

    Google Scholar 

  14. M. Yamaguchi, F. Miyamaru, K. Yamamoto, M. Tani, and M. Hangyo, “Terahertz absorption spectra of L-, D-, and DL-alanine and their application to determination of enantiometric composition,” Appl. Phys. Lett. 86(5), 1–3 (2005).

    Google Scholar 

  15. N. Li, J. L. Shen, J. H. Sun, L. S. Liang, X. Y. Xu, M. H. Lu, and Y. Jia, “Study on the THz spectrum of methamphetamine,” Opt. Express 13(18), 6750–6755 (2005).

    Article  Google Scholar 

  16. T. Ikeda, A. Matsushita, M. Tatsuno, Y. Minami, M. Yamaguchi, K. Yamamoto, M. Tani, and M. Hangyo, “Investigation of inflammable liquids by terahertz spectroscopy,” Appl. Phys. Lett. 87(3), 034105:1-3 (2005).

    Article  Google Scholar 

  17. K. Yamamoto, M. Yamaguchi, M. Tani, M. Hangyo, S. Teramura, T. Isu, and N. Tomita, “Degradation diagnosis of ultrahigh-molecular weight polyethylene with terahertz-time-domain spectroscopy,” Appl. Phys. Lett. 85(22), 5194–5196 (2004).

    Article  Google Scholar 

  18. P. C. Ashworth, E. Pickwell-MacPherson, E. Provenzano, S. E. Pinder, A. D. Purushotham, M. Pepper, and V. P. Wallace, “Terahertz pulsed spectroscopy of freshly excised human breast cancer,” Opt. Express 17(15), 12444-12454 (2009).

    Article  Google Scholar 

  19. H. Chen, T. H. Chen, T. F. Tseng, J. T. Lu, C. C. Kuo, S. C. Fu, W. J. Lee, Y. F. Tsai, Y. Y. Huang, E. Y. Chuang, Y. J. Hwang, and C. K. Sun, “High-sensitivity in vivo THz transmission imaging of early human breast cancer in a subcutaneous xenograft mouse model,” Opt. Express 19(22), 21552-21562 (2011).

    Article  Google Scholar 

  20. H. Chen, S. H. Ma, W. X. Yan, X. M. Wu, and X. Z. Wang, “The diagnosis of human liver cancer by using THz fiber-scanning near-field imaging,” Chin. Phys. Lett. 30(3), 030702:1–3 (2013).

    Article  Google Scholar 

  21. P. Huang, Y. Cao, J. Chen, W. Ge, D. Hou, and G. Zhang, “Analysis and inspection techniques for mouse liver injury based on terahertz spectroscopy,” Opt. Express 27(18), 26014-26026 (2019).

    Article  Google Scholar 

  22. R. M. Woodward, V. P. Wallace, D. D. Arnone, E. H. Linfield, and M. Pepper, “Terahertz pulsed imaging of skin cancer in the time and frequency domain,” J. Biol. Phys. 29(2–3), 257–259 (2003).

    Article  Google Scholar 

  23. R. M. Woodward, B. E. Cole, V. P. Wallace, R. J. Pye, D. D. Arnone, E. H. Linfield, and M. Pepper, “Terahertz pulse imaging in reflection geometry of human skin cancer and skin tissue,” Phys. Med. Biol. 47(21), 3853–3863 (2002).

    Article  Google Scholar 

  24. G. M. Png, J. W. Choi, B. W. H. Ng, S. P. Mickan, D. Abbott, and X. C. Zhang, “The impact of hydration changes in fresh bio-tissue on THz spectroscopic measurements,” Phys. Med. Biol. 53(13), 3501–3517 (2008).

    Article  Google Scholar 

  25. W. Withayachumnankul and M. Naftaly, “Fundamentals of measurement in terahertz time-domain spectroscopy,” J. Infrared Millim. Te. 35(8), 610–637 (2014).

    Article  Google Scholar 

  26. I. Pupeza, R. Wilk, and M. Koch, “Highly accurate optical material parameter determination with THz time-domain spectroscopy,” Opt. Express 15(7), 4335-4350 (2007).

    Article  Google Scholar 

  27. L. I. Jiusheng, “Optical parameters of vegetable oil studied by terahertz time-domain spectroscopy,” Appl. Spectrosc. 64(2), 231–234 (2010).

    Article  Google Scholar 

  28. S. Sy, S. Huang, Y. X. J. Wang, J. Yu, A. T. Ahuja, Y. T. Zhang, and E. Pickwell-MacPherson, “Terahertz spectroscopy of liver cirrhosis: Investigating the origin of contrast,” Phys. Med. Biol. 55(24), 7587–7596 (2010).

    Article  Google Scholar 

  29. A. K. Akobeng, “Understanding diagnostic tests 1: Sensitivity, specificity and predictive values,” Acta Paediatr. 96(3), 338–341 (2007).

    Article  Google Scholar 

  30. D. G. Altman and J. M. Bland, “Statistics Notes: Diagnostic tests 1: Sensitivity and specificity,” Br. Med. J. 308(6943), 1552 (1994).

    Article  Google Scholar 

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Funding

This study was supported by a Joint Research Project between Southeast University and Nanjing Medical University (No. 3207027381).

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Authors and Affiliations

  1. School of Physics, Southeast University, Nanjing, 211189, Jiangsu, China

    Yu Zhang, Juan Han, Dan Wang & Hua Chen

  2. The First Affiliated Hospital, Nanjing Medical University, Nanjing, 210029, Jiangsu, China

    Xiao Li, Tianzhu Qiu & Xiaofeng Chen

Authors
  1. Yu Zhang
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  2. Juan Han
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  3. Dan Wang
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  4. Xiao Li
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  5. Tianzhu Qiu
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  6. Hua Chen
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  7. Xiaofeng Chen
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Correspondence to Hua Chen.

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Zhang, Y., Han, J., Wang, D. et al. Application of THz Time-Domain Spectroscopy to Diagnose Gastric Cancer Tissues in Surgical Resected Specimens. J Infrared Milli Terahz Waves 42, 802–812 (2021). https://doi.org/10.1007/s10762-021-00805-7

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  • DOI: https://doi.org/10.1007/s10762-021-00805-7

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