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Periodontitis May Restrain the Mandibular Bone Healing via Disturbing Osteogenic and Osteoclastic Balance

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Abstract

Periodontitis has been advocated as a systematic chronic low-grade infection burden. However, the relationship between periodontitis and bone defect healing has not been elucidated. One hundred and eight male Wister rats were randomly assigned into three groups: control (healthy) group, periodontitis group, and periodontitis plus human tumor necrosis factor receptorII:IgG Fc fusion protein (rhTNFR:Fc) group. The experimental periodontitis model was established by ligaturing with orthodontic wire and silk suture plus local administration of 20 μl of lipopolysaccharide (LPS). Mandibular bone defects in size of 4 × 2 × 1 mm were created for all the rats and rhTNFR:Fc subcutaneously injected at neck at a dose of 2.5 mg/kg every 3 days for the periodontitis plus rhTNFR:Fc group. The gene and protein expressions of bone-related markers in the healing tissue were monitored and new bone formation was histologically evaluated. Tartrate-resistant acid phosphatase (TRAP) staining was performed to determine the number of osteoclasts. The results showed that the mRNA and protein expressions of osteogenesis-related markers were significantly lower while nuclear factor of activated T cells, cytoplasmic 1 (NFATc1) gene expression was significantly higher in the periodontitis group. The periodontitis group showed decreased new bone formation and increased number of osteoclasts when compared to the control group. However, there was no significant difference between the periodontitis plus rhTNFR:Fc group and the control group. These results demonstrated that periodontitis may restrain the mandibular bone healing via disturbing osteogenic and osteoclastic balance in which tumor necrosis factor-α (TNF-α) could act as a leverage.

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Funding

This work was supported by the National Natural Science Foundation of China (Grant No. 81771076), Beijing, China and the Construction Engineering Special Fund of “Taishan Scholars,” Jinan, China.

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Authors and Affiliations

  1. Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Shandong University, Jinan, Shandong, China

    Rongjing Zhou & Pishan Yang

  2. Liaocheng People’s Hospital, Liaocheng, Shandong, China

    Rongjing Zhou & Lili Shen

  3. Department of Oral & Maxillofacial Surgery, Qilu Hospital and Institute of Stomatology, Shandong University, Jinan, Shandong, China

    Chengzhe Yang

  4. Department of Stomatology, Qilu Hospital and Institute of Stomatology, Shandong University, Jinan, Shandong, China

    Limei Wang

  5. Department of Periodontology, School of Dentistry, Shandong University, Jinan, Shandong, 250000, China

    Hongmei Guo, Pishan Yang & Aimei Song

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  1. Rongjing Zhou
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  2. Lili Shen
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Correspondence to Pishan Yang or Aimei Song.

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This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Zhou, R., Shen, L., Yang, C. et al. Periodontitis May Restrain the Mandibular Bone Healing via Disturbing Osteogenic and Osteoclastic Balance. Inflammation 41, 972–983 (2018). https://doi.org/10.1007/s10753-018-0751-5

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  • DOI: https://doi.org/10.1007/s10753-018-0751-5

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