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Burn-Induced Multiple Organ Injury and Protective Effect of Lutein in Rats

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Abstract

Thermal injury may lead to multiple organ dysfunction through release of proinflammatory mediators and reactive oxygen radicals. This study investigated the effects of thermal injury on remote organs of rats and the possible protective effect of lutein. Thermal trauma was induced in the back of rats by exposing them to 90 °C bath for 10 s. Rats were sacrificed 48 h after burn, and blood samples were collected to monitor liver and kidney functions. Tissue samples from liver, kidneys, and lungs were taken for studying oxidative stress parameters, gene expressions of TNF-α and Casp-3, besides histopathological examination. Skin scald injury caused significant elevations of liver and kidney function biomarkers in the serum. In tissue samples, increments of MDA, GPx, and 8-OHdG were recorded while GSH level and the activities of CAT and SOD were suppressed. The expressions of TNF-α and caspase-3 mRNA were increased, and histopathological results revealed remote organ injury. Oral administration of lutein (250 mg/kg) resulted in amelioration of the biochemical and molecular changes induced by burn as well as the histopathological alterations. According to the findings of the present study, lutein possesses anti-oxidant, anti-inflammatory, and anti-apoptotic effects that protect against burn-induced damage in remote organs.

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Acknowledgements

We thank the consultation of the central laboratory in the Faculty of Veterinary Medicine, Cairo University as we had bench space to work.

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Authors and Affiliations

  1. Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt

    Huda O. AbuBakr & Samira H. Aljuaydi

  2. Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, University of Sadat City, Sadat, Egypt

    Shimaa M. Abou-Zeid

  3. Department of Pathology, Faculty of Veterinary Medicine, University of Sadat City, Sadat, Egypt

    Amanallah El-Bahrawy

Authors
  1. Huda O. AbuBakr
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  2. Samira H. Aljuaydi
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  3. Shimaa M. Abou-Zeid
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  4. Amanallah El-Bahrawy
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Contributions

Huda O. AbuBakr and Samira H. Aljuaydi have participated in performing biochemical analysis of oxidant/anti-oxidant biomarkers and gene expression. Shimaa M. Abou-Zeid has performed the experimental design and participated in biochemical analysis of liver and kidney functions and oxidative stress. Amanallah El-Bahrawy has performed histopathological examination. All authors have contributed in writing this article. All authors critically read and revised the manuscript and approved its submission for publication.

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Correspondence to Huda O. AbuBakr.

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Ethics approval and consent to participate this study were approved by the Animal Use and Care Committee at Faculty of Veterinary Medicine, University of Sadat City, Egypt.

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AbuBakr, H.O., Aljuaydi, S.H., Abou-Zeid, S.M. et al. Burn-Induced Multiple Organ Injury and Protective Effect of Lutein in Rats. Inflammation 41, 760–772 (2018). https://doi.org/10.1007/s10753-018-0730-x

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