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Tenuigenin Prevents IL-1β-induced Inflammation in Human Osteoarthritis Chondrocytes by Suppressing PI3K/AKT/NF-κB Signaling Pathway

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Abstract

Tenuigenin (TEN), the main active component of Polygala tenuifolia, has been reported to have anti-inflammatory effects. However, the effects of TEN on IL-1β-stimulated osteoarthritis chondrocytes have not been reported. The purpose of this study was to investigate the anti-inflammatory effects and mechanism of TEN on IL-1β-stimulated human osteoarthritis chondrocytes. Human osteoarthritis chondrocytes were pretreated with or without TEN for 1 h and then stimulated with IL-1β. The production of NO and PGE2 were detected by the Griess reagent and ELISA. The expression of NF-κB and MAPKs (p38, JNK, ERK) were measured by Western blot analysis. The production of MMP-1, MMP3, and MMP13 were measured by ELISA. The results showed that treatment of TEN significantly inhibited IL-1β-induced NO and PGE2 production. TEN also suppressed IL-1β-induced MMP-1, MMP3, and MMP13 expression. Furthermore, TEN was found to inhibit IL-1β-induced NF-κB activation, PI3K, and AKT phosphorylation. In conclusion, these results suggest that TEN inhibits IL-1β-induced inflammation in human osteoarthritis chondrocytes by inhibiting PI3K/AKT/NF-κB signaling pathway.

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Authors and Affiliations

  1. Department of Orthopedics, the First Affiliated Hospital of Harbin Medical University, Harbin, 150001, China

    Chunlei Wang, Tao Zhang, Jiakun Liu & Wenbo Wang

  2. Harbin Children Hospital, Harbin, 150010, China

    Lihong Zeng

Authors
  1. Chunlei Wang
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  2. Lihong Zeng
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  3. Tao Zhang
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  4. Jiakun Liu
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  5. Wenbo Wang
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Correspondence to Wenbo Wang.

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This experiment was conducted in accordance with the Declaration of Helsinki and Tokyo.

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The authors declare that they have no competing interests.

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Wang, C., Zeng, L., Zhang, T. et al. Tenuigenin Prevents IL-1β-induced Inflammation in Human Osteoarthritis Chondrocytes by Suppressing PI3K/AKT/NF-κB Signaling Pathway. Inflammation 39, 807–812 (2016). https://doi.org/10.1007/s10753-016-0309-3

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  • DOI: https://doi.org/10.1007/s10753-016-0309-3

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