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Anti-inflammatory Steroid from Phragmitis rhizoma Modulates LPS-Mediated Signaling Through Inhibition of NF-κB Pathway

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ABSTRACT

Lipopolysaccharides (LPS) strongly stimulate immune cells, and unabated activation of immune system by LPS may lead to an exacerbation of sickness and depression. In this study, stigmasta-3,5-dien-7-one (ST) was isolated from Phragmitis rhizoma as a negative regulator of LPS-induced inflammation in macrophages. ST effectively reduced nitric oxide (NO), prostaglandin E2, and pro-inflammatory cytokine levels, which were markedly raised by LPS treatment. In addition, ST blocked the nuclear factor-kappa B (NF-κB) signaling pathway via down-regulation of phospho-p38 mitogen-activated protein kinase and phosphorylation and degradation of the inhibitor of NF-κB α. To our knowledge, this is the first study showing anti-inflammatory activities of ST isolated from Phragmitis rhizoma.

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ACKNOWLEDGMENTS

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No.2012R1A5A2A42671316) and partly by the Technology R&D Program funded by the Small and Medium Business Administration (SMBA, Korea).

Conflict of Interest

The authors declare that they have no competing interests.

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    Authors and Affiliations

    1. Medical Research Center for Globalization of Herbal Formulation, College of Korean Medicine, Daegu Haany University, Gyeongsan, Republic of Korea

      Sook Jahr Park, Young Woo Kim, Sung Hui Byun & Sang Chan Kim

    2. Department of Pharmaceutical Engineering, Daegu Haany University, 1 Haanydaero, Gyeongsan, 38610, Republic of Korea

      Moon Ki Park & Jong Rok Lee

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    1. Sook Jahr Park
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    2. Young Woo Kim
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    Correspondence to Jong Rok Lee.

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    Sook Jahr Park and Young Woo Kim contributed equally to this work.

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    Park, S.J., Kim, Y.W., Park, M.K. et al. Anti-inflammatory Steroid from Phragmitis rhizoma Modulates LPS-Mediated Signaling Through Inhibition of NF-κB Pathway. Inflammation 39, 727–734 (2016). https://doi.org/10.1007/s10753-015-0299-6

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    • DOI: https://doi.org/10.1007/s10753-015-0299-6

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