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Alpha-Lipoic Acid Attenuates Oxidative Damage in Organs After Sepsis

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Abstract

Sepsis progression is linked with the imbalance between reactive oxygen species and antioxidant enzymes. Thus, the aim of this study was to evaluate the effect of alpha-lipoic acid (ALA), a powerful antioxidant, in organs of rats submitted to sepsis. Male Wistar rats were subjected to sepsis by cecal ligation puncture (CLP) and treated with ALA or vehicle. After CLP (12 and 24 h), the myeloperoxidase (MPO) activity, protein and lipid oxidative damage, and antioxidant enzymes in the liver, kidney, heart, and lung were evaluated. ALA was effective in reducing MPO activity, lipid peroxidation in the liver, and protein carbonylation only in the kidney in 12 h after CLP. In 12 h, SOD activity increased in the kidney and CAT activity in the liver and kidney with ALA treatment. Thus, ALA was able to reduce the inflammation and oxidative stress in the liver and kidney after sepsis in rats.

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Authors and Affiliations

  1. Clinical and Experimental Pathophysiology Laboratory—FICEXP, Graduate Program in Health Sciences, University of South Santa Catarina, Avenida José Acácio Moreira, 787, 88704-900, Tubarão, SC, Brazil

    Fabricia Petronilho, Drielly Florentino, Lucinéia Gainski Danielski, Luiz Carlos Vieira, Maryane Modolon Martins, Andriele Vieira, Sandra Bonfante & Mariana Pereira Goldim

  2. Experimental Pathophysiology Laboratory—FISIOPAT, Graduate Program in Health Sciences, University of Southern of Santa Catarina, Criciuma, SC, Brazil

    Francieli Vuolo

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  1. Fabricia Petronilho
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Petronilho, F., Florentino, D., Danielski, L.G. et al. Alpha-Lipoic Acid Attenuates Oxidative Damage in Organs After Sepsis. Inflammation 39, 357–365 (2016). https://doi.org/10.1007/s10753-015-0256-4

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