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Inhibitory Effect of Orientin on Secretory Group IIA Phospholipase A2

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Abstract

It is well known that the expression level of secretory group IIA phospholipase A2 (sPLA2-IIA) is elevated in inflammatory diseases and lipopolysaccharide (LPS) upregulates the expression of sPLA2-IIA in human umbilical vein endothelial cells (HUVECs). Orientin, a C-glycosyl flavonoid, is known to have anxiolytic, anti-oxidative, and anti-inflammatory activity. Here, orientin was examined for its effects on the expression and activity of sPLA2-IIA in HUVECs and mouse. Prior treatment of cells or mouse with orientin inhibited LPS-induced expression and activity of sPLA2-IIA. And orientin suppressed the activation of cytosolic phospholipase A2 (cPLA2) and extracellular signal-regulated kinase (ERK) 1/2 by LPS. Therefore, these results suggest that orientin may inhibit LPS-mediated expression of sPLA2-IIA by suppression of cPLA2 and ERK 1/2.

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Acknowledgments

This research was supported by the National Research Foundation of Korea (NRF) funded by the Korea government [MSIP] (Grant Nos. 2014R1A2A1A11049526).

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  1. College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu, 702-701, Republic of Korea

    Jong-Sup Bae

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Bae, JS. Inhibitory Effect of Orientin on Secretory Group IIA Phospholipase A2 . Inflammation 38, 1631–1638 (2015). https://doi.org/10.1007/s10753-015-0139-8

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