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Patchouli Alcohol Dampens Lipopolysaccharide Induced Mastitis in Mice

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Abstract

Patchouli alcohol (PA), a tricyclic sesquiterpene isolated from Pogostemonis Herba, has been known to exhibit antioxidant, anti-inflammatory, and other important therapeutic activities. The aim of this study was to investigate the effects of PA on LPS-induced mastitis in vivo and the possible mechanism. The mouse model of mastitis was induced by injection of LPS through the duct of mammary gland. Mice were pretreated with dexamethasone or PA 1 h before and 12 h after induction of LPS. The myeloperoxidase activity and inflammatory cytokines production in mammary tissues were determined. The effects of PA on NF-κB signal pathways were analyzed by Western blotting. The results showed that PA inhibited the LPS-induced TNF-α, IL-6, and IL-1β production in a dose manner. It was also observed that PA attenuated mammary histopathologic changes. Furthermore, Western blot analysis showed that PA could inhibit the phosphorylation of NF-κB and IκB induced by LPS. These results indicate that PA inhibits NF-κB signaling pathways to attenuate inflammatory injury induced by LPS. PA may be a potent therapeutic reagent for the prevention of mastitis.

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References

  1. Jain, N.C. 1979. Common mammary pathogens and factors in infection and mastitis. Journal of Dairy Science 62: 128–134.

    Article  PubMed  CAS  Google Scholar 

  2. Seegers, H., C. Fourichon, and F. Beaudeau. 2003. Production effects related to mastitis and mastitis economics in dairy cattle herds. Veterinary Research 34: 475–491.

    Article  PubMed  Google Scholar 

  3. Hortet, P., and H. Seegers. 1998. Loss in milk yield and related composition changes resulting from clinical mastitis in dairy cows. Preventive Veterinary Medicine 37: 1–20.

    Article  PubMed  CAS  Google Scholar 

  4. Burvenich, C., V. Van Merris, J. Mehrzad, A. Diez-Fraile, and L. Duchateau. 2003. Severity of E-coli mastitis is mainly determined by cow factors. Veterinary Research 34: 521–564.

    Article  PubMed  Google Scholar 

  5. Vels, L., C.M. Rontved, M. Bjerring, and K.L. Ingvartsen. 2009. Cytokine and acute phase protein gene expression in repeated liver biopsies of dairy cows with a lipopolysaccharide-induced mastitis. Journal of Dairy Science 92: 922–934.

    Article  PubMed  CAS  Google Scholar 

  6. Rambeaud, M., R.A. Almeida, G.M. Pighetti, and S.P. Oliver. 2003. Dynamics of leukocytes and cytokines during experimentally induced Streptococcus uberis mastitis. Veterinary Immunology and Immunopathology 96: 193–205.

    Article  PubMed  CAS  Google Scholar 

  7. Jeong, J.B., J. Choi, Z.Y. Lou, X.J. Jiang, and S.H. Lee. 2013. Patchouli alcohol, an essential oil of Pogostemon cablin, exhibits anti-tumorigenic activity in human colorectal cancer cells. International Immunopharmacology 16: 184–190.

    Article  PubMed  CAS  Google Scholar 

  8. Wu, X.L., D.H. Ju, J. Chen, B. Yu, K.L. Liu, J.X. He, C.Q. Dai, S. Wu, Z. Chang, Y.P. Wang, and X.Y. Chen. 2013. Immunologic mechanism of patchouli alcohol anti-H1N1 influenza virus may through regulation of the RLH signal pathway in vitro. Current Microbiology 67: 431–436.

    Article  PubMed  CAS  Google Scholar 

  9. Jeong, J.B., Y.K. Shin, and S.H. Lee. 2013. Anti-inflammatory activity of patchouli alcohol in RAW264.7 and HT-29 cells. Food and Chemical Toxicology 55: 229–233.

    Article  PubMed  CAS  Google Scholar 

  10. Xian, Y.F., Y.C. Li, S.P. Ip, Z.X. Lin, X.P. Lai, and Z.R. Su. 2011. Anti-inflammatory effect of patchouli alcohol isolated from Pogostemonis Herba in LPS-stimulated RAW264.7 macrophages. Experimental and Therapeutic Medicine 2: 545–550.

    PubMed  CAS  PubMed Central  Google Scholar 

  11. Li, Y.C., Y.F. Xian, S.P. Ip, Z.R. Su, J.Y. Su, J.J. He, Q.F. Xie, X.P. Lai, and Z.X. Lin. 2011. Anti-inflammatory activity of patchouli alcohol isolated from Pogostemonis Herba in animal models. Fitoterapia 82: 1295–1301.

    Article  PubMed  CAS  Google Scholar 

  12. Li, D., Y. Fu, W. Zhang, G. Su, B. Liu, M. Guo, F. Li, D. Liang, Z. Liu, X. Zhang, et al. 2013. Salidroside attenuates inflammatory responses by suppressing nuclear factor-kappaB and mitogen activated protein kinases activation in lipopolysaccharide-induced mastitis in mice. Inflammation Research 62: 9–15.

    Article  PubMed  CAS  Google Scholar 

  13. Owens, W.E., C.H. Ray, J.L. Watts, and R.J. Yancey. 1997. Comparison of success of antibiotic therapy during lactation and results of antimicrobial susceptibility tests for bovine mastitis. Journal of Dairy Science 80: 313–317.

    Article  PubMed  CAS  Google Scholar 

  14. Katz, S.E., and M.S. Brady. 2000. Antibiotic residues in food and their significance (reprinted from Antimicrobials in Foods, pg 571–595, 1993). Food Biotechnology 14: 147–171.

    Article  CAS  Google Scholar 

  15. Sordillo, L.M., and K.L. Streicher. 2002. Mammary gland immunity and mastitis susceptibility. Journal of Mammary Gland Biology and Neoplasia 7: 135–146.

    Article  PubMed  Google Scholar 

  16. Mehrzad, J., H. Dosogne, E. Meyer, R. Heyneman, and C. Burvenich. 2001. Respiratory burst activity of blood and milk neutrophils in dairy cows during different stages of lactation. Journal of Dairy Research 68: 399–415.

    Article  PubMed  CAS  Google Scholar 

  17. Krawisz, J.E., P. Sharon, and W.F. Stenson. 1984. Quantitative assay for acute intestinal inflammation based on myeloperoxidase activity. Assessment of inflammation in rat and hamster models. Gastroenterology 87: 1344–1350.

    PubMed  CAS  Google Scholar 

  18. Waller, K.P., I.G. Colditz, S. Lun, and K. Ostensson. 2003. Cytokines in mammary lymph and milk during endotoxin-induced bovine mastitis. Research in Veterinary Science 74: 31–36.

    Article  Google Scholar 

  19. Hoeben, D., C. Burvenich, E. Trevisi, G. Bertoni, J. Hamann, R.M. Bruckmaier, and J.W. Blum. 2000. Role of endotoxin and TNF-alpha in the pathogenesis of experimentally induced coliform mastitis in periparturient cows. Journal of Dairy Research 67: 503–514.

    Article  PubMed  CAS  Google Scholar 

  20. Nakajima, Y., O. Mikami, M. Yoshioka, Y. Motoi, T. Ito, Y. Ishikawa, M. Fuse, K. Nakano, and K. Yasukawa. 1997. Elevated levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) activities in the sera and milk of cows with naturally occurring coliform mastitis. Research in Veterinary Science 62: 297–298.

    Article  PubMed  CAS  Google Scholar 

  21. Donnahoo, K.K., X. Meng, A. Ayala, M.P. Cain, A.H. Harken, and D.R. Meldrum. 1999. Early kidney TNF-alpha expression mediates neutrophil infiltration and injury after renal ischemia-reperfusion. American Journal of Physiology 277: R922–R929.

    PubMed  CAS  Google Scholar 

  22. Buraczynska, M., P. Ksiazek, P. Kubit, and W. Zaluska. 2006. Interleukin-1 receptor antagonist gene polymorphism affects the progression of chronic renal failure. Cytokine 36: 167–172.

    Article  PubMed  CAS  Google Scholar 

  23. Xing, Z., J. Gauldie, G. Cox, H. Baumann, M. Jordana, X.F. Lei, and M.K. Achong. 1998. IL-6 is an antiinflammatory cytokine required for controlling local or systemic acute inflammatory responses. Journal of Clinical Investigation 101: 311–320.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  24. Miao, J.F., Y.M. Zhu, B.B. Gu, X.B. Wang, S.X. Zou, and Y.E. Deng. 2007. Evaluation of the changes of immune cells during lipopolysaccharide-induced mastitis in rats. Cytokine 40: 135–143.

    Article  PubMed  CAS  Google Scholar 

  25. Tang, E.D., C.Y. Wang, Y. Xiong, and K.L. Guan. 2003. A role for NF-kappaB essential modifier/IkappaB kinase-gamma (NEMO/IKKgamma) ubiquitination in the activation of the IkappaB kinase complex by tumor necrosis factor-alpha. Journal of Biological Chemistry 278: 37297–37305.

    Article  PubMed  CAS  Google Scholar 

  26. Gilmore, T.D. 2006. Introduction to NF-kappaB: players, pathways, perspectives. Oncogene 25: 6680–6684.

    Article  PubMed  CAS  Google Scholar 

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Conflict of Interest

All authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, 15 West Changle Road, Xi’an, Shan Xi, 710032, China

    Yong-Ping Li, Shi-Fang Yuan, Hui Wang, Ling Wang, Rui Ling & Jun Yun

  2. Department of Anatomy, Histology and Embryology & K.K. Leung Brain Research Centre, Preclinical School of Medicine, Fourth Military Medical University, Xi’an, Shan Xi, 710032, China

    Guo-Hong Cai

  3. Department of Biopharmaceutical Key Laboratory of Heilongjiang Province, College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang, 150081, China

    Lei Yu

Authors
  1. Yong-Ping Li
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  2. Shi-Fang Yuan
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  3. Guo-Hong Cai
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  4. Hui Wang
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  5. Ling Wang
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  6. Lei Yu
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  7. Rui Ling
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  8. Jun Yun
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Corresponding authors

Correspondence to Rui Ling or Jun Yun.

Additional information

Yong-Ping Li, Shi-Fang Yuan, and Guo-Hong Cai contributed equally to this work.

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Li, YP., Yuan, SF., Cai, GH. et al. Patchouli Alcohol Dampens Lipopolysaccharide Induced Mastitis in Mice. Inflammation 37, 1757–1762 (2014). https://doi.org/10.1007/s10753-014-9905-2

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  • DOI: https://doi.org/10.1007/s10753-014-9905-2

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