Skip to main content
SpringerLink
Log in

The Effect of Taurine on the Relationship Between NO, ADMA and Homocysteine in Endotoxin-Mediated Inflammation in HUVEC Cultures

  • Published:
Inflammation Aims and scope Submit manuscript

Abstract

The aim of our study was to investigate the effect of taurine on the relationship between nitric oxide (NO), asymmetric dimethylarginine (ADMA) and homocysteine (Hcy) in endotoxin-induced human umblical vein endothelial cell (HUVEC) cultures. For this reason, four groups were formed (n = 12). Control group consists of HUVEC cultures without any treatment. Lipopolysaccharide (LPS) and LPS + taurine groups were treated with 10 μg/mL endotoxin, 5 μg/mL taurine and endotoxin + taurine (same doses), respectively. Nitrite/nitrate (NOx), ADMA and Hcy levels were measured. There was a significant increase of NOx, ADMA and Hcy in endotoxemia (p < 0.05). Taurine treatment elevated NOx levels significantly (p < 0.01) in taurine and LPS + taurine group compared to control group, while it reduced NOx levels compared to LPS group. In contrast, taurine decreased ADMA levels to the control level both in taurine and taurine + LPS group compared to LPS. Hcy levels increased significantly compared to taurine group (p < 0.05) and did not change compared to LPS group. Taurine was effective on ADMA–NO relationship whereas no beneficial effect was observed in Hcy levels (p < 0.05).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. Cristina de Assis, M., M. Cristina Plotkowski, I.M. Fierro, C. Barja-Fidalgo, and M.S. de Freitas. 2002. Expression of inducible nitric oxide synthase in human umbilical vein endothelial cells during primary culture. Nitric Oxide 7(4): 254–261.

    Article  PubMed  CAS  Google Scholar 

  2. Laurindo, F.R., A. Pedro Mde, H.V. Barbeiro, F. Pileggi, M.H. Carvalho, O. Augusto, and P.L. da Luz. 1994. Vascular free radical release. Ex vivo and in vivo evidence for a flow-dependent endothelial mechanism. Circulation Research 74(4): 700–709.

    Article  PubMed  CAS  Google Scholar 

  3. Jia, Y.X., C.S. Pan, J.H. Yang, X.H. Liu, W.J. Yuan, J. Zhao, C.S. Tang, and Y.F. Qi. 2006. Altered l-arginine/nitric oxide synthase/nitric oxide pathway in the vascular adventitia of rats with sepsis. Clinical and Experimental Pharmacology and Physiology 33(12): 1202–1208.

    Article  PubMed  CAS  Google Scholar 

  4. Davignon, J., and P. Ganz. 2004. Role of endothelial dysfunction in atherosclerosis. Circulation 109(23 Suppl 1): III27–III32.

    PubMed  Google Scholar 

  5. Moncada, S., and A. Higgs. 1993. The L-arginine-nitric oxide pathway. The New England Journal of Medicine 329(27): 2002–2012.

    Article  PubMed  CAS  Google Scholar 

  6. Wells, S.M., and A. Holian. 2007. Asymmetric dimethylarginine induces oxidative and nitrosative stress in murine lung epithelial cells. American Journal of Respiratory Cell and Molecular Biology 36(5): 520–528.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  7. Bhagat, K., R. Moss, J. Collier, and P. Vallance. 1996. Endothelial “stunning” following a brief exposure to endotoxin: a mechanism to link infection and infarction? Cardiovascular Research 32(5): 822–829.

    PubMed  CAS  Google Scholar 

  8. Tan, B., D.J. Jiang, H. Huang, S.J. Jia, J.L. Jiang, C.P. Hu, and Y.J. Li. 2007. Taurine protects against low-density lipoprotein-induced endothelial dysfunction by the DDAH/ADMA pathway. Vascular Pharmacology 46(5): 338–345.

    Article  PubMed  CAS  Google Scholar 

  9. MacAllister, R.J., S.A. Fickling, G.S. Whitley, and P. Vallance. 1994. Metabolism of methylarginines by human vasculature; implications for the regulation of nitric oxide synthesis. British Journal of Pharmacology 112(1): 43–48.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  10. Schulze, F., H. Lenzen, C. Hanefeld, A. Bartling, K.J. Osterziel, L. Goudeva, C. Schmidt-Lucke, M. Kusus, R. Maas, E. Schwedhelm, D. Strödter, B.C. Simon, A. Mügge, W.G. Daniel, H. Tillmanns, B. Maisch, T. Streichert, and R.H. Böger. 2006. Asymmetric dimethylarginine is an independent risk factor for coronary heart disease: results from the multicenter Coronary Artery Risk Determination investigating the Influence of ADMA Concentration (CARDIAC) study. American Heart Journal 152(3): 493.e1–493.e8.

    Article  Google Scholar 

  11. Vasan, R.S., A. Beiser, R.B. D’Agostino, D. Levy, J. Selhub, P.F. Jacques, I.H. Rosenberg, and P.W. Wilson. 2003. Plasma homocysteine and risk for congestive heart failure in adults without prior myocardial infarction. JAMA 289(10): 1251–1257.

    Article  PubMed  CAS  Google Scholar 

  12. Herrmann, M., I. Kindermann, S. Müller, T. Georg, M. Kindermann, M. Böhm, and W. Herrmann. 2005. Relationship of plasma homocysteine with the severity of chronic heart failure. Clinical Chemistry 51(8): 1512–1515.

    Article  PubMed  CAS  Google Scholar 

  13. Hoffman, M. 2011. Hypothesis: hyperhomocysteinemia is an indicator of oxidant stress. Medical Hypotheses 77(6): 1088–1093.

    Article  PubMed  CAS  Google Scholar 

  14. Böger, R.H., S.R. Lentz, S.M. Bode-Böger, H.R. Knapp, and W.G. Haynes. 2001. Elevation of asymmetrical dimethylarginine may mediate endothelial dysfunction during experimental hyperhomocyst(e)inaemia in humans. Clinical Science (London) 100(2): 161–167.

    Article  Google Scholar 

  15. Egan, B.M., H. Abdih, C.J. Kelly, C. Condron, and D.J. Bouchier-Hayes. 2001. Effect of intravenous taurine on endotoxin-induced acute lung injury in sheep. The European Journal of Surgery 167(8): 575–580.

    Article  PubMed  CAS  Google Scholar 

  16. Erdamar, H., N. Türközkan, B. Balabanli, G. Ozan, and F.S. Bircan. 2007. The relationship between taurine and 3-nitrotyrosine level of hepatocytes in experimental endotoxemia. Neurochemical Research 32(11): 1965–1968.

    Article  PubMed  CAS  Google Scholar 

  17. Bhavsar, T.M., S.N. Patel, and C.A. Lau-Cam. 2010. Protective action of taurine, given as a pretreatment or as a posttreatment, against endotoxin-induced acute lung inflammation in hamsters. Journal of Biomedical Science 17(Suppl 1): S19.

    Article  PubMed  PubMed Central  Google Scholar 

  18. Ekremoğlu, M., N. Türközkan, H. Erdamar, Y. Kurt, and H. Yaman. 2007. Protective effect of taurine on respiratory burst activity of polymorphonuclear leukocytes in endotoxemia. Amino Acids 32(3): 413–417.

    Article  PubMed  Google Scholar 

  19. Baudin, B., A. Bruneel, N. Bosselut, and M. Vaubourdolle. 2007. A protocol for isolation and culture of human umbilical vein endothelial cells. Nature Protocols 2(3): 481–485.

    Article  PubMed  CAS  Google Scholar 

  20. Bachetti, T., L. Comini, G. Francolini, D. Bastianon, B. Valetti, M. Cadei, P. Grigolato, H. Suzuki, D. Finazzi, A. Albertini, S. Curello, and R. Ferrari. 2004. Arginase pathway in human endothelial cells in pathophysiological conditions. Journal of Molecular and Cellular Cardiology 37(2): 515–523.

    Article  PubMed  CAS  Google Scholar 

  21. Bories, P.N., and C. Bories. 1995. Nitrate determination in biological fluids by an enzymatic one-step assay with nitrate reductase. Clinical Chemistry 41(6 Pt 1): 904–907.

    PubMed  CAS  Google Scholar 

  22. Araki, A., and Y. Sako. 1987. Determination of free and total homocysteine in human plasma by high-performance liquid chromatography with fluorescence detection. Journal of Chromatography 422: 43–52.

    Article  PubMed  CAS  Google Scholar 

  23. Chen, B.M., L.W. Xia, and R.Q. Zhao. 1997. Determination of N(G),N(G)-dimethylarginine in human plasma by high-performance liquid chromatography. Journal of Chromatography B: Biomedical Sciences and Applications 692(2): 467–471.

    Article  PubMed  CAS  Google Scholar 

  24. Fennessy, F.M., D.S. Moneley, J.H. Wang, C.J. Kelly, and D.J. Bouchier-Hayes. 2003. Taurine and vitamin C modify monocyte and endothelial dysfunction in young smokers. Circulation 107(3): 410–415.

    Article  PubMed  CAS  Google Scholar 

  25. Ulrich-Merzenich, G., H. Zeitler, H. Vetter, and R.R. Bhonde. 2007. Protective effects of taurine on endothelial cells impaired by high glucose and oxidized low density lipoproteins. European Journal of Nutrition 46(8): 431–438.

    Article  PubMed  CAS  Google Scholar 

  26. Stühlinger, M.C., P.S. Tsao, J.H. Her, M. Kimoto, R.F. Balint, and J.P. Cooke. 2001. Homocysteine impairs the nitric oxide synthase pathway: role of asymmetric dimethylarginine. Circulation 104(21): 2569–2575.

    Article  PubMed  Google Scholar 

  27. Kenyon, S.H., C.J. Waterfield, J.A. Timbrell, and A. Nicolaou. 2002. Methionine synthase activity and sulphur amino acid levels in the rat liver tumour cells HTC and Phi-1. Biochemical Pharmacology 63(3): 381–391.

    Article  PubMed  CAS  Google Scholar 

  28. Anand, P., D. Rajakumar, M. Jeraud, A.J. Felix, and T. Balasubramanian. 2011. Effects of taurine on glutathione peroxidase, glutathione reductase and reduced glutathione levels in rats. Pakistan Journal of Biological Sciences 14(3): 219–225.

    Article  PubMed  CAS  Google Scholar 

Download references

ACKNOWLEDGMENTS

Gazi University Scientific Research Project Unit supports this project.

Author information

Authors and Affiliations

  1. Department of Medical Biochemistry, Faculty of Medicine, Gazi University, Ankara, Turkey

    Ozge Tugce Pasaoglu & Nurten Turkozkan

  2. Department of Pharmacology, Faculty of Pharmacy, Gazi University, Ankara, Turkey

    Mustafa Ark

  3. Department of Perinatology, Zekai Tahir Burak Women’s Health Education and Research Hospital, Ankara, Turkey

    Belgin Polat

  4. Department of Clinical Biochemistry, Gulhane Military Medicine Academy, 06018, Ankara, Turkey

    Mehmet Agilli & Halil Yaman

Authors
  1. Ozge Tugce Pasaoglu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nurten Turkozkan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mustafa Ark
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Belgin Polat
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mehmet Agilli
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Halil Yaman
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Halil Yaman.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pasaoglu, O.T., Turkozkan, N., Ark, M. et al. The Effect of Taurine on the Relationship Between NO, ADMA and Homocysteine in Endotoxin-Mediated Inflammation in HUVEC Cultures. Inflammation 37, 1439–1443 (2014). https://doi.org/10.1007/s10753-014-9868-3

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10753-014-9868-3

KEY WORDS

Search

Navigation