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Shock Wave Induces Biological Renal Damage by Activating Excessive Inflammatory Responses in Rat Model

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Abstract

The study was aimed to investigate the potential mechanism of inflammatory renal damage induced by shock wave. A total of 48 rats, with the right kidney cut, are randomly assigned into control group, ESWL group and ESWL + PDTC group. Rats were treated with shock wave at the left kidney. At post-shock wave 3 and 105 days, all the animals were sacrificed for detecting the expression of tumor necrosis factor (TNF)-α, intercellular adhesion molecule (ICAM)-1, and monocyte chemoattractant protein (MCP)-1. The inflammatory responses were evaluated by detecting the level of myeloperoxidase (MPO) and ED-1. The histological renal injury was also examined. Before the animals were sacrificed, the urine samples were collected for measuring the values of malondialdehyde (MDA), β2-microglobulin, interleukin (IL)-6, and IL-18. At post-shock wave 3 days, the higher expression of ICAM-1 and TNF-α were observed in shock wave-treated kidneys. The level of urine TNF-α, IL-6, and IL-18 were also increased significantly. Using PDTC obviously decreased the expression of ICAM-1 and TNF-α. It also effectively inhibited the degree of oxidative stress and neutrophil infiltration. At post-shock wave 105 days, the expression of MCP-1 and the level of urine β2-microglobulin and IL-18 were increased significantly. The histological analysis also indicated more ED-1-positive cells and serious fibrosis in shock wave-treated kidneys. PDTC significantly suppressed MCP-1 and IL-18 expression, decreased monocyte infiltration, and alleviate the degree of interstitium fibrosis. Shock wave triggered excessive inflammatory responses and aggravated renal biological damage. Several inflammatory factors including ICAM-1, MCP-1, and TNF-α were considered to play important role in this type of renal damage.

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Acknowledgment

This work was supported by the National Natural Science Foundation of China (Grant No. 30700833).

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Authors and Affiliations

  1. Department of Urology, First Affiliated Hospital, Medical College, Xi’an Jiaotong University, No. 277 Yanta West Road, Xi’an, Shaanxi Province, 710061, China

    Xiang Li, Qingzhi Long, Xinfa Cheng & Dalin He

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  1. Xiang Li
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  2. Qingzhi Long
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  3. Xinfa Cheng
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  4. Dalin He
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Correspondence to Dalin He.

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Li, X., Long, Q., Cheng, X. et al. Shock Wave Induces Biological Renal Damage by Activating Excessive Inflammatory Responses in Rat Model. Inflammation 37, 1317–1325 (2014). https://doi.org/10.1007/s10753-014-9859-4

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  • DOI: https://doi.org/10.1007/s10753-014-9859-4

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