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Protective Effect of p-Cymene on Lipopolysaccharide-Induced Acute Lung Injury in Mice

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Abstract

In the previous study, the anti-inflammatory effect of p-cymene had been found. In this study, we investigated anti-inflammatory effects of p-cymene on acute lung injury using lipopolysaccharide (LPS)-induced acute lung injury (ALI) mouse model. The cell counting in the bronchoalveolar lavage fluid (BALF) was measured. The animal lung edema degree was evaluated by wet/dry weight (W/D) ratio. The superoxidase dismutase (SOD) activity and myeloperoxidase (MPO) activity was assayed by SOD and MPO kits, respectively. The levels of inflammatory mediators including tumor necrosis factor alpha (TNF-α), IL-1β, and IL-6 were assayed by enzyme-linked immunosorbent assay method. The pathological changes of the lung tissues were observed by hematoxylin and eosin staining. The inflammatory signal pathway-related protein levels of NF-κB were measured using Western blotting. The data showed that treatment with the p-cymene markedly attenuated inflammatory cell numbers in the BALF, decreased NF-κB protein level in the lungs, improved SOD activity, and inhibited MPO activity. Histological studies demonstrated that p-cymene substantially inhibited LPS-induced neutrophils in the lung tissue compared with the model group. The results indicated that p-cymene had a protective effect on LPS-induced ALI in mice.

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Acknowledgments

The project was supported by Chinese National Project of “Twelfth Five-Year” Plan for Science & Technology Support (2012BAI27B06), the Special Fund for Basic Scientific Research of Central Colleges, South-Central University for Nationalities (no. CZY13016), the Natural Science Foundation of China (81303284) and the Planning of Deeply Participating into the Enterprises for Young Teachers (XD2012060).

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Authors and Affiliations

  1. College of Pharmacy, South-Central University for Nationalities, Wuhan, 430074, People’s Republic of China

    Lvyi Chen

  2. Key Laboratory of Chemistry and Quality for Traditional Chinese Medicines of the College of Gansu Province, Gansu College of Traditional Chinese Medicine, Lanzhou, 730000, People’s Republic of China

    Lei Zhao

  3. Key Laboratory of Modern Chinese Medicines, China Pharmaceutical University, Ministry of Education, Nanjing, 210009, People’s Republic of China

    Chunfeng Zhang

  4. School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, 430065, People’s Republic of China

    Zhou Lan

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  1. Lvyi Chen
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  2. Lei Zhao
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Correspondence to Chunfeng Zhang or Zhou Lan.

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Chen, L., Zhao, L., Zhang, C. et al. Protective Effect of p-Cymene on Lipopolysaccharide-Induced Acute Lung Injury in Mice. Inflammation 37, 358–364 (2014). https://doi.org/10.1007/s10753-013-9747-3

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  • DOI: https://doi.org/10.1007/s10753-013-9747-3

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