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Urinary Trypsin Inhibitor Attenuated Inflammatory Response of Patients Undergoing Cardiopulmonary Bypass by Inducing Activated Treg Cells

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ABSTRACT

The urinary trypsin inhibitor (ulinastatin) is used in the clinic to prevent inflammatory responses in patients undergoing cardiopulmonary bypass (CPB); however, the anti-inflammatory mechanism is unclear. In the current study, we recruited 40 patients undergoing selective cardiac valve replacement surgery; and these patients were randomly divided into two groups (ulinastatin group [UG] and control group [CG]). We collected peripheral blood preoperatively, at the end of CPB, and postoperative days 1 and 3 and analyzed the kinetic changes in regulatory T (Treg) cell subsets. There was no statistically significant difference in the number of CD4+ T cells between the two groups. The number of CD4+CD25+ Treg cells, especially the suppressive activated Treg (aTreg) subset, was higher in the UG than the CG 1 and 3 days postoperatively. Thus, ulinastatin alleviated the inflammatory response during CPB by inducing the expansion of aTreg cells.

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Abbreviations

CPB:

Cardiopulmonary bypass

TNF-α:

Tumor necrosis factor-alpha

IL:

Interleukin

AAo:

Ascending aorta

CRP:

C-reactive protein

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ACKNOWLEDGMENTS

This work was supported by grants from the National Natural Science Foundation of China (nos. 81070203 and 81071587) and the Foundation for Medical Science of Beijing (no. 2009-3114).

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None.

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Authors and Affiliations

  1. Department of Extracorporeal Circulation, Center for Cardiac Intensive Care, Beijing Anzhen Hospital, Capital Medical University, No. 2 Anding Road, Chaoyang District, Beijing, 100029, China

    Xing Hao, Zhichen Xing, Chunjing Jiang, Jing Yang & Xiaotong Hou

  2. Beijing Key Laboratory of Emerging Infectious Diseases, Beijing, 100015, China

    Junyan Han, Yu Hao & Jianping Zhang

  3. Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing, 100015, China

    Junyan Han, Yu Hao & Jianping Zhang

Authors
  1. Xing Hao
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  2. Junyan Han
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  3. Zhichen Xing
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  4. Yu Hao
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  5. Chunjing Jiang
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  6. Jianping Zhang
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  8. Xiaotong Hou
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Correspondence to Xiaotong Hou.

Additional information

Xing Hao and Junyan Han contributed equally to this work.

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Hao, X., Han, J., Xing, Z. et al. Urinary Trypsin Inhibitor Attenuated Inflammatory Response of Patients Undergoing Cardiopulmonary Bypass by Inducing Activated Treg Cells. Inflammation 36, 1279–1285 (2013). https://doi.org/10.1007/s10753-013-9666-3

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  • DOI: https://doi.org/10.1007/s10753-013-9666-3

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