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Rehmannia glutinosa Suppresses Inflammatory Responses Elicited by Advanced Glycation End Products

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Abstract

Fresh rhizome of Rehmannia glutinosa Libosch. (Saeng-jihwang in Korean: SJH) has been prescribed for the treatment of diabetes-associated complications. The purpose of the present study is to investigate the underlying mechanisms of the efficacy of SJH in diabetes-related complications. Decoction was obtained after boiling SJH in water and subsequent lyophilization. The cellular toxicity of SJH was determined by MTT assay. The antioxidant activity of SJH was measured by DPPH and DCFH-DA assays. The effects of SJH on inflammatory responses elicited by AGEs were assessed by western blotting and semi-quantitative RT-PCR analyses. The water extract of SJH had a high free radical scavenging activity in vitro and decreased the level of intracellular ROS in THP-1 cells treated with AGEs. SJH suppressed the expression of pro-inflammatory genes, including TNF-α, MCP-1, IP-10, COX-2, and iNOS; the activation of NF-κB; and the expression of RAGE, a receptor for AGEs, where the expressions of which were induced by AGEs. These results suggest the possibility that SJH can be an alternative therapeutics for diabetes-associated diseases.

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Acknowledgments

This work was in part supported by a grant from Ministry of Education Science and Technology KGM2250911 (M.J.).

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Authors and Affiliations

  1. Department of Molecular Biology, Chonbuk National University, Jeonju, 561-756, Republic of Korea

    Gui-Hyun Baek & Yong-Suk Jang

  2. Jeonju Biomaterials Institute, Jeonju, 561-360, Republic of Korea

    Seung-Il Jeong

  3. Department of Microbiology, College of Natural Sciences, Pusan National University, Busan, 609-735, Republic of Korea

    Jaeho Cha

  4. Division of Applied Medicine, School of Korean Medicine, Pusan National University, Yangsan, 626-870, Republic of Korea

    Myungsoo Joo, Sang-Woo Shin, Ki-Tae Ha & Han-Sol Jeong

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  1. Gui-Hyun Baek
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Baek, GH., Jang, YS., Jeong, SI. et al. Rehmannia glutinosa Suppresses Inflammatory Responses Elicited by Advanced Glycation End Products. Inflammation 35, 1232–1241 (2012). https://doi.org/10.1007/s10753-012-9433-x

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