Skip to main content
SpringerLink
Log in

The Effects of the Combination of Sodium Ferulate and Oxymatrine on Lipopolysaccharide-Induced Acute Lung Injury in Mice

  • Published:
Inflammation Aims and scope Submit manuscript

Abstract

In our previous study, the remarkable analgesic and anti-inflammatory effects of the combination of sodium ferulate (SF) and oxymatrine (OMT) had been found. In this study, we investigated the effect of the combination of SF and OMT on acute lung injury using lipopolysaccharide (LPS)-induced acute lung injury (ALI) mouse model. The cell counting and the protein concentration in the bronchoalveolar lavage fluid (BALF) were measured. The animal lung edema degree was evaluated by wet/dry weight (W/D) ratio. The superoxidase dismutase (SOD) activity and myeloperoxidase (MPO) activity was assayed by SOD and MPO kits, respectively. The levels of inflammatory mediators including C-reactive protein (CRP) and tumor necrosis factor-α (TNF-α) were assayed by enzyme-linked immunosorbent assay method. The data showed that treatment with the combination of SF and OMT markedly attenuated inflammatory cell numbers and protein concentration in the BALF and improved SOD activity and inhibited MPO activity compared to LPS group. Moreover, the combination significantly inhibited the production of CRP and TNF-α in lung homogenate. The histological changes of the lungs were also more significantly improved by the combination. At the same dose, the obvious protective effect was not found in SF or OMT-treated alone group except that the protein concentration slightly decreased in SF group. The results indicated that the combination SF and OMT had a protective effect on LPS-induced ALI in mice, and the effect was much better than that of SF or OMT used alone.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Bersten, A.D., C. Edibam, T. Hunt, and J. Moran. 2002. Incidence andmortality of acute lung injury and the acute respiratory distress syndrome in three Australian states. American Journal of Respiratory and Critical Care Medicine 165: 443–448.

    PubMed  Google Scholar 

  2. Chau, C.S., N.G. Michelle, Z. Rihong, C. Feng, K. Ednan, and E.K. Bajwa. 2010. Clinical characteristics and outcomes of sepsis-related vs non-sepsis-related ARDS. American College of Chest Physicians 138: 559–567.

    Google Scholar 

  3. Chen, Z., X. Zhang, X. Chu, X. Zhang, K. Song, Y. Jiang, L. Yu, and X. Deng. 2010. Preventive effects of Valnemulin on Lipopolysaccharide-induced acute lung injury in mice. Inflammation 33: 306–314.

    Article  PubMed  CAS  Google Scholar 

  4. Chan, M.H., P.K. Chan, J.F. Griffith, and I.H. Chan. 2006. Steroid-induced osteonecrosis in severe acute respiratory syndrome: a retrospective analysis of biochemical markers of bone metabolism and corticosteroid therapy. Pathology 38(3): 229–235.

    Article  PubMed  CAS  Google Scholar 

  5. Liu, H.Q., Y. Sun, Y. Gao, F.F. Chen, M.B. Xu, and Z.F. Liu. 2010. The analgesic effect and mechanism of the combination of sodium ferulate and oxymatrine. Neurochem Research 35: 1368–1375.

    Article  CAS  Google Scholar 

  6. Yuan, X.P., Y. Sun, N.S. Miao, S.Y. Sun, Y.Y. Wang, Z. Hu, and J. Yuan. 2011. The synergistic anti-inflammatory effect of the combination of sodium ferulate and oxymatrine and its modulation on inflammation-associated mediators in RAW 264.7 cells. Journal of Ethnopharmacology 137:1477–1485.

    Google Scholar 

  7. Itoh, T., H. Obata, S. Murakami, K. Hamada, K. Kangawa, H. Kimura, and N. Nagaya. 2007. Adrenomedullin ameliorates lipopolysaccharide-induced acute lung injury in rats. American Journal of Physiology 293: L446–L452.

    PubMed  CAS  Google Scholar 

  8. Speyer, C.L., T.A. Neff, R.L. Warner, R.F. Guo, J.V. Sarma, N.C. Riedemann, M.E. Murphy, H.S. Murphy, and P.A. Ward. 2003. Regulatory effects of iNOS on acute lung inflammatory responsesin mice. American Journal of Pathology 163: 2319–2328.

    Article  PubMed  CAS  Google Scholar 

  9. Kang, J.L., H.W. Lee, H.S. Lee, I.S. Pack, Y. Chong, and V. Castranova. 2001. Genistein prevents nuclear factor-kappa B activation and acute lung injury induced by lipopolysaccharide. American Journal of Respiratory and Critical Care Medicine 164: 2206–2212.

    PubMed  CAS  Google Scholar 

  10. Arsalane, K., F. Broeckaert, B. Knoops, M. Wiedig, G. Toubeau, and A. Bernard. 2000. Clara cell specific protein (CC16) expression after acute lung inflammation induced by intratracheal lipopolysaccharide administration. American Journal of Respiratory and Critical Care Medicine 161: 1624–1630.

    PubMed  CAS  Google Scholar 

  11. Hong, B.B., W.J. Cheol, L. Mei, S.K. Hyung, and H.K. Sang. 2011. Effects of urinary trypsin inhibitor on lipopolysaccharide-induced acute lung injury in rabbits. Inflammation (online first).

  12. Matute, B.G., R.K. Winn, M. Jonas, E.Y. Chi, T.R. Martin, and W.C. Liles. 2001. Fas (CD95) induces alveolar epithelial cell apoptosis in vivo: Implications for acute pulmonary inflammation. American Journal of Pathology 158: 153.

    Article  Google Scholar 

  13. Wang, B.H., and J.P. Ou-Yang. 2005. Pharmacological actions of sodium ferulate in cardiovascular system. Cardiovascular Drug Review 23: 161–172.

    Article  Google Scholar 

  14. Xu, G.L., L. Yao, S.Y. Rao, Z.N. Gong, S.Q. Zhang, and S.Q. Yu. 2005. Attenuation of acute lung injury in mice by oxymatrine is associated with inhibition of phosphorylated p38 mitogen-activated protein kinase. Journal of Ethnopharmacology 98: 177–183.

    Article  PubMed  CAS  Google Scholar 

  15. Szarka, R.J., N. Wang, L. Gordon, P.N. Nation, and R.H. Smith. 1997. A murine model of pulmonary damage induced by lipopolysaccharide via intranasal instillation. Journal of Immunological Methods 202: 49–57.

    Article  PubMed  CAS  Google Scholar 

  16. Xu, X.L., Q.M. Xie, Y.H. Shen, J.J. Jiang, Y.Y. Chen, H.Y. Yao, and J.Y. Zhou. 2008. Mannose prevents lipopolysaccharide-induced acute lung injury in rats. Inflammation Research 57: 104–110.

    Article  PubMed  CAS  Google Scholar 

  17. Klebanoff, S.J. 2005. Myeloperoxidase: friend and foe. Journal of Leukocyte Biology 77: 598–625.

    Article  PubMed  CAS  Google Scholar 

  18. Winterbourn, C.C., M.C. Vissers, and A.J. Kettle. 2000. Myeloperoxidase. Current Opinion in Hematology 7: 53–58.

    Article  PubMed  CAS  Google Scholar 

  19. Meduri, G.U., S. Headley, G. Kohler, et al. 1995. Persistent elevation of inflammatory cytokines predicts a poor outcome in ARDS. Plasma IL-1 beta and IL-6 levels are consistent and efficient predictors of outcome over time. The Cardiopulmonary and Critical Care Journal 107: 1062–1073.

    CAS  Google Scholar 

  20. Ballou, S.P., and G. Lozanski. 1992. Induction of inflammatory cytokine release from cultured human monocytes by C-reactive protein. Cytokine 4: 361–368.

    Article  PubMed  CAS  Google Scholar 

  21. Cermak, J., N.S. Key, R.R. Bach, J. Balla, H.S. Jacob, and G.M. Vercellotti. 1993. C-reactive protein induces human peripheral blood monocytes to synthesize tissue factor. Blood 82: 513–520.

    PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We thank Dr. Peter Zhang of MediAlliance Inc for his valuable comments and Dr. Yujun Li for the pathological analysis. This work was supported with the grants of the innovative strategy plan of significant new drugs researches program from Ministry of Science and Technology of the People’s Republic of China (program no. 2008ZX09101-Z-017) and the equipment promoting program from Department of Science and Technology of Shangdong Province (program no. 2007GG10002026).

Author information

Authors and Affiliations

  1. School of Pharmacy, Yantai University, No. 30, Qingquan Road, Laishan District, Yantai, Shandong, 264005, People’s Republic of China

    Xiaopei Yuan, Yuyun Wang, Zhao Hu, Mengxin Xu & Zhifeng Liu

  2. The Laishan Branch Hospital of Yuhuanding Hospital, No. 1181 Gangcheng Avenue, Laishan district, Yantai, Shandong, 264003, People’s Republic of China

    Dunren Du

  3. Beijing SL Pharmaceutical Co., Ltd., No. 9, Shijingshan Industrial Area, Haidian District, Beijing, 100041, People’s Republic of China

    Mingbo Xu

  4. Director of Basic Medical Department, School of Pharmacy, Yantai University, No. 30, Qingquan Road, Laishan District, Yantai, 100041, People’s Republic of China

    Zhifeng Liu

Authors
  1. Xiaopei Yuan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yuyun Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dunren Du
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zhao Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mengxin Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Mingbo Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Zhifeng Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhifeng Liu.

Additional information

X. Yuan and Y. Wang contributed equal work for this study.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yuan, X., Wang, Y., Du, D. et al. The Effects of the Combination of Sodium Ferulate and Oxymatrine on Lipopolysaccharide-Induced Acute Lung Injury in Mice. Inflammation 35, 1161–1168 (2012). https://doi.org/10.1007/s10753-011-9424-3

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10753-011-9424-3

KEY WORDS

Search

Navigation