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Fractalkine Upregulates Inflammation through CX3CR1 and the Jak–Stat Pathway in Severe Acute Pancreatitis Rat Model

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Abstract

Based on the function of chemokine fractalkine (FKN), acting as both adhesion and chemoattractant, FKN plays a role in acute inflammatory response. In this study, we investigated the mechanism of FKN mediated upregulation inflammation in severe acute pancreatitis (SAP) rat models. Western blot, reverse transcriptase-polymerase chain reaction, and immunofluorescence demonstrated that FKN and its receptor CX3CR1 were overexpressed in cerulein-stimulated AR42J cells. AG490 and FKN-siRNA inhibited activation of Janus kinase/signal transducers and activators of transcription (Jak/Stat) in cerulein-stimulated AR42J cells. Following exposure AG490 and FKN-siRNA inhibited tumor necrosis factor-alpha expression by enzyme-linked immunosorbent assay and immunohistochemistry in vivo the SAP rat models. These results showed FKN and CX3CR1 were involved inflammatory response in cerulein-stimulated AR42J cells. FKN upregulates inflammation through CX3CR1 and the Jak/Stat pathway in SAP rat models.

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Author notes

  1. Li-ya Huang

    Present address: Department of Gastroenterology, Affiliated Hospital of Ningxia Medical University, Yinchuan, 750004, Ningxia Hui Autonomous Region, China

Authors and Affiliations

  1. Department of Gastroenterology, Ruijin Hospital Shanghai Jiaotong University School of Medicine, Shanghai, 200025, China

    Ping Chen, Ling-xiao Xu, Yu-fen Zhou, Yong-ping Zhang & Yao-zong Yuan

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  1. Li-ya Huang
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  2. Ping Chen
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  3. Ling-xiao Xu
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Correspondence to Yao-zong Yuan.

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Huang, Ly., Chen, P., Xu, Lx. et al. Fractalkine Upregulates Inflammation through CX3CR1 and the Jak–Stat Pathway in Severe Acute Pancreatitis Rat Model. Inflammation 35, 1023–1030 (2012). https://doi.org/10.1007/s10753-011-9406-5

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