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Sec14l3 Is Specifically Expressed in Mouse Airway Ciliated Cells

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Abstract

Airway epithelium is a key component for airway integrity. Previously, we found that expression of the Sec14l3 gene that encodes a 45-kDa secretory protein is inversely associated with the progression of experimentally induced airway inflammation and degeneration/necrosis of alveolar epithelium. In this report, using in situ hybridization we demonstrated that the ciliated cells in mouse lung selectively express Sec14l3 mRNA. In a three-dimensional culture of mouse tracheal epithelial cells, levels of the Sec14l3 mRNA correlated with the differentiation of ciliated cells. Intranasal infection of adult mice with influenza virus resulted in a 20-fold, progressive decrease in Sec14l3 mRNA expression over 10 days post infection. These results enhance the potential value of Sec14l3 as a ciliated epithelial cell-specific biomarker for the progression of airway inflammations such as airway viral infection and asthma.

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ACKNOWLEDGEMENTS

The authors would like to thank Drs. Tomoyuki Yokota and Mine Kinoshita, Tsukuba Research Laboratories (TRL), GlaxoSmithKline K.K., Japan, for their support and helpful discussion during this work.

Conflict of Interest Statement

There is no potential conflict of interest in this manuscript.

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Author notes

  1. Keizo Yamashita

    Present address: Kaizen and Excellence in Hospital Operations, Tsukuba University Hospital, Ibaraki, Japan

Authors and Affiliations

  1. GlaxoSmithKline K.K., Tokyo, Japan

    Lihua Shan, Shinobu Noritake, Masatoshi Fujiwara, Satoshi Asano, Nobuhiro Noro, Keizo Yamashita & Takao Kawakami

  2. Faculty of Pharmacy, Tianjin University of Traditional Chinese Medicine, Tianjin, China

    Lihua Shan

  3. Ribomic Inc., Tokyo, Japan

    Masatoshi Fujiwara

  4. Department of Pharmaceutical Sciences, International University of Health and Welfare, Tochigi, Japan

    Satoshi Asano

  5. College of Industrial Technology, Advanced Research Institute for the Science and Humanities and School of Medicine, Nihon University, Tokyo, Japan

    Chikako Yoshida-Noro

  6. Department of Surgery, Tokyo Medical University, 6-7-1 Nishi Shinjuku, Tokyo, 160-0023, Japan

    Takao Kawakami

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  1. Lihua Shan
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Correspondence to Takao Kawakami.

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Shan, L., Noritake, S., Fujiwara, M. et al. Sec14l3 Is Specifically Expressed in Mouse Airway Ciliated Cells. Inflammation 35, 702–712 (2012). https://doi.org/10.1007/s10753-011-9363-z

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